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4.1.2.10: (R)-mandelonitrile lyase

This is an abbreviated version!
For detailed information about (R)-mandelonitrile lyase, go to the full flat file.

Word Map on EC 4.1.2.10

Reaction

(R)-mandelonitrile
=
cyanide
 +
benzaldehyde

Synonyms

(R)-(+)-Mandelonitrile lyase, (R)-HNL, (R)-HNL5, (R)-hydroxynitrile lyase, (R)-Mandelonitrile lyase, (R)-Oxynitrilase, (R)-PeHNL, (R)-selective HNL, AciX9_0562, almond oxynitrilase, APHNL, AtHNL, cupin 2 conserved barrel domain protein, cupin 2 domain-containing protein, D-alpha-hydroxynitrile lyase, D-Hydroxynitrile lyase, D-Oxynitrilase, DtHNL1, EjHNL, FAD-HNL, HNL, HNL1, HNL2, HNL4, HNL5, Hydroxynitrile lyase, hydroynitrile lyase, Lyase, mandelonitrile, mandelonitrile lyase, MDL, Mdl1, NdHNL, Oxynitrilase, PaHNL, PaHNL1, PaHNL5, PaMDL, ParsHNL, PhaMDL, PmHNL, R-HNL, R-hydroxynitrile lyase, R-selective HNL, R-selective hydroxynitrile lyase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.2 Aldehyde-lyases
                4.1.2.10 (R)-mandelonitrile lyase

Purification

Purification on EC 4.1.2.10 - (R)-mandelonitrile lyase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation
Prunus pseudoarmeniaca
-
ammonium sulfate precipitation and Ni Sepharose column chromatography
ammonium sulfate precipitation, DEAE Toyopearl column chromatography, phenyl Toyopearl column chromatography, and Superdex 200 gel filtration
hydroxynitrile lyase isoenzymes (HNL1, HNL2, HNL4 and HNL5)
-
mutant enzyme V317A
partial
-
partly, as crosslinked enzyme aggregates. Optimal conditions are initial glutaraldehyde concentration of 25% w/v, ammonium sulfate saturation concentration of 43% w/v, and crosslinking time of 18 h
-
recombinant enzyme
-
the fused family 2 carbohydrate-binding module enables the purification and simultaneous immobilization of Arabidopsis thaliana hydroxynitrile lyase on various cellulosic carrier materials
to homogeneity. The final yield, of 36% with 49fold purification, is obtained by 30-80% (NH4)2SO4 fractionation and column chromatography on DEAE-Toyopearl and concanavalin A Sepharose 4B, which suggests the presence of a carbohydrate side chain