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Literature summary for 4.1.2.10 extracted from

  • Ueatrongchit, T.; Tamura, K.; Ohmiya, T.; H-Kittikun, A.; Asano, Y.
    Hydroxynitrile lyase from Passiflora edulis. Purification, characteristics and application in asymmetric synthesis of (R)-mandelonitrile (2010), Enzyme Microb. Technol., 46, 456-465.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15000
-
1 * 15000, SDS-PAGE Passiflora edulis
18000
-
gel filtration Passiflora edulis

Organic Solvent Stability

Organic Solvent Comment Organism
dibutyl ether more than 80% residual activity after incubation for 12 h in the system of methyl-tert-butyl ether, dibutyl ether, hexane, and diisopropyl ether while diethyl ether and ethyl acetate are not suitable solvents Passiflora edulis
diethyl ether more than 80% residual activity after incubation for 12 h in the system of methyl-tert-butyl ether, dibutyl ether, hexane, and diisopropyl ether while diethyl ether and ethyl acetate are not suitable solvents Passiflora edulis
diisopropyl ether more than 80% residual activity after incubation for 12 h in the system of methyl-tert-butyl ether, dibutyl ether, hexane, and diisopropyl ether while diethyl ether and ethyl acetate are not suitable solvents Passiflora edulis
Ethyl acetate more than 80% residual activity after incubation for 12 h in the system of methyl-tert-butyl ether, dibutyl ether, hexane, and diisopropyl ether while diethyl ether and ethyl acetate are not suitable solvents Passiflora edulis
hexane more than 80% residual activity after incubation for 12 h in the system of methyl-tert-butyl ether, dibutyl ether, hexane, and diisopropyl ether while diethyl ether and ethyl acetate are not suitable solvents Passiflora edulis
methyl-tert-butylether more than 80% residual activity after incubation for 12 h in the system of methyl-tert-butyl ether, dibutyl ether, hexane, and diisopropyl ether while diethyl ether and ethyl acetate are not suitable solvents Passiflora edulis

Organism

Organism UniProt Comment Textmining
Passiflora edulis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Passiflora edulis

Source Tissue

Source Tissue Comment Organism Textmining
fruit rind Passiflora edulis
-
leaf
-
Passiflora edulis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
136
-
-
Passiflora edulis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyanide + 2,2-dimethylpropanal activity is 33% of the activity with benzaldehyde Passiflora edulis (2R)-2-hydroxy-3,3-dimethylbutanenitrile 9% enentiomeric excess ?
cyanide + 2-methylpropanal activity is 67% of the activity with benzaldehyde Passiflora edulis (2R)-2-hydroxy-3-methylbutanenitrile 13% enentiomeric excess ?
cyanide + benzaldehyde several parameters influence the enantiomeric purity of the product and initial velocity of the reaction. Both pH and temperature are important parameters controlling the enantiomeric purity of the product. The optimum pH and temperature are pH 4 and 10°C, respectively. At the optimum pH and temperature, the spontaneous non-enzymatic reaction yielding the racemic mandelonitrile is almost completely suppressed. The initial velocity is markedly affected by the type of organic solvent in the biphasic system, while high enantiomeric purity is obtained when organic solvents having log P lower than 3.5 are used. The highest initial velocity of reaction and enantiomeric purity of (R)-mandelonitrile are obtained in the biphasic system of dibutyl ether with the aqueous phase content of 30% (v/v). The optimum substrate concentrations are 250 mM for benzaldehyde and 900 mM for acetone cyanohydrin, and the optimum enzyme concentration is 26.7 units/ml. The highest enantiomeric purity of (R)-mandelonitrile is successfully obtained with conversion and enantiomeric excess of 31.6% and 98.6%, respectively Passiflora edulis (R)-mandelonitrile
-
?
cyanide + cyclohexanecarbaldehyde activity is 41% of the activity with benzaldehyde Passiflora edulis (2R)-cyclohexyl(hydroxy)acetonitrile 10% enentiomeric excess ?
cyanide + propanal activity is 20% of the activity with benzaldehyde Passiflora edulis (2R)-2-hydroxybutanenitrile 7% enentiomeric excess ?
cyanide + thiophene-2-carbaldehyde activity is 2fold higher than with benzaldehyde Passiflora edulis (2S)-hydroxy(thiophen-2-yl)ethanenitrile 75% enentiomeric excess, The (S)-configuration is due to the Cahn-Ingold-Prelog rules ?
additional information activity is less than 5% of the activity with benzaldehyde: 4-methoxybenzaldehyde, naphthalene-1-carbaldehyde, naphthalene-2-carbaldehyde and 1,3-benzodioxole-5-carbaldehyde Passiflora edulis ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 15000, SDS-PAGE Passiflora edulis

Synonyms

Synonyms Comment Organism
(R)-PeHNL
-
Passiflora edulis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
10
-
selected as the optimum temperature for the reaction to avoid the non-enzymatic reaction producing racemates of mandelonitrile Passiflora edulis
40
-
optimal temperature, but also high non-enzymatic reaction Passiflora edulis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
selected as the assay temperature for the reaction to avoid the non-enzymatic reaction producing racemates of mandelonitrile Passiflora edulis
6
-
optimal pH, but also high non-enzymatic reaction Passiflora edulis