Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15000 | - |
1 * 15000, SDS-PAGE | Passiflora edulis |
18000 | - |
gel filtration | Passiflora edulis |
Organic Solvent | Comment | Organism |
---|---|---|
dibutyl ether | more than 80% residual activity after incubation for 12 h in the system of methyl-tert-butyl ether, dibutyl ether, hexane, and diisopropyl ether while diethyl ether and ethyl acetate are not suitable solvents | Passiflora edulis |
diethyl ether | more than 80% residual activity after incubation for 12 h in the system of methyl-tert-butyl ether, dibutyl ether, hexane, and diisopropyl ether while diethyl ether and ethyl acetate are not suitable solvents | Passiflora edulis |
diisopropyl ether | more than 80% residual activity after incubation for 12 h in the system of methyl-tert-butyl ether, dibutyl ether, hexane, and diisopropyl ether while diethyl ether and ethyl acetate are not suitable solvents | Passiflora edulis |
Ethyl acetate | more than 80% residual activity after incubation for 12 h in the system of methyl-tert-butyl ether, dibutyl ether, hexane, and diisopropyl ether while diethyl ether and ethyl acetate are not suitable solvents | Passiflora edulis |
hexane | more than 80% residual activity after incubation for 12 h in the system of methyl-tert-butyl ether, dibutyl ether, hexane, and diisopropyl ether while diethyl ether and ethyl acetate are not suitable solvents | Passiflora edulis |
methyl-tert-butylether | more than 80% residual activity after incubation for 12 h in the system of methyl-tert-butyl ether, dibutyl ether, hexane, and diisopropyl ether while diethyl ether and ethyl acetate are not suitable solvents | Passiflora edulis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Passiflora edulis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Passiflora edulis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | rind | Passiflora edulis | - |
leaf | - |
Passiflora edulis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
136 | - |
- |
Passiflora edulis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyanide + 2,2-dimethylpropanal | activity is 33% of the activity with benzaldehyde | Passiflora edulis | (2R)-2-hydroxy-3,3-dimethylbutanenitrile | 9% enentiomeric excess | ? | |
cyanide + 2-methylpropanal | activity is 67% of the activity with benzaldehyde | Passiflora edulis | (2R)-2-hydroxy-3-methylbutanenitrile | 13% enentiomeric excess | ? | |
cyanide + benzaldehyde | several parameters influence the enantiomeric purity of the product and initial velocity of the reaction. Both pH and temperature are important parameters controlling the enantiomeric purity of the product. The optimum pH and temperature are pH 4 and 10°C, respectively. At the optimum pH and temperature, the spontaneous non-enzymatic reaction yielding the racemic mandelonitrile is almost completely suppressed. The initial velocity is markedly affected by the type of organic solvent in the biphasic system, while high enantiomeric purity is obtained when organic solvents having log P lower than 3.5 are used. The highest initial velocity of reaction and enantiomeric purity of (R)-mandelonitrile are obtained in the biphasic system of dibutyl ether with the aqueous phase content of 30% (v/v). The optimum substrate concentrations are 250 mM for benzaldehyde and 900 mM for acetone cyanohydrin, and the optimum enzyme concentration is 26.7 units/ml. The highest enantiomeric purity of (R)-mandelonitrile is successfully obtained with conversion and enantiomeric excess of 31.6% and 98.6%, respectively | Passiflora edulis | (R)-mandelonitrile | - |
? | |
cyanide + cyclohexanecarbaldehyde | activity is 41% of the activity with benzaldehyde | Passiflora edulis | (2R)-cyclohexyl(hydroxy)acetonitrile | 10% enentiomeric excess | ? | |
cyanide + propanal | activity is 20% of the activity with benzaldehyde | Passiflora edulis | (2R)-2-hydroxybutanenitrile | 7% enentiomeric excess | ? | |
cyanide + thiophene-2-carbaldehyde | activity is 2fold higher than with benzaldehyde | Passiflora edulis | (2S)-hydroxy(thiophen-2-yl)ethanenitrile | 75% enentiomeric excess, The (S)-configuration is due to the Cahn-Ingold-Prelog rules | ? | |
additional information | activity is less than 5% of the activity with benzaldehyde: 4-methoxybenzaldehyde, naphthalene-1-carbaldehyde, naphthalene-2-carbaldehyde and 1,3-benzodioxole-5-carbaldehyde | Passiflora edulis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 15000, SDS-PAGE | Passiflora edulis |
Synonyms | Comment | Organism |
---|---|---|
(R)-PeHNL | - |
Passiflora edulis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | - |
selected as the optimum temperature for the reaction to avoid the non-enzymatic reaction producing racemates of mandelonitrile | Passiflora edulis |
40 | - |
optimal temperature, but also high non-enzymatic reaction | Passiflora edulis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
selected as the assay temperature for the reaction to avoid the non-enzymatic reaction producing racemates of mandelonitrile | Passiflora edulis |
6 | - |
optimal pH, but also high non-enzymatic reaction | Passiflora edulis |