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0
pH 5.0, wild-type, half-life 18 min, surface-modified mutant, half-life 137 min
100
-
1 h, complete loss of activity
15 - 40
1 h, no loss of activity
22
-
the relative activity of free enzyme decreases rapidly and depletes at the end of 15 days storage time while the immobilized hydroxynitrile lyases are very active at the same conditions with retained initial activities of 67% and 83% for hydroxynitrile lyase immobilized onto Eupergit CM and hydroxynitrile lyase immobilized onto Eupergit C 250 L, respectively. At the end of 30 days storage time, the corresponding retained activities are 48% and 71% for hydroxynitrile lyase immobilized onto Eupergit CM and hydroxynitrile lyase immobilized onto Eupergit C 250 L
25 - 50
Prunus pseudoarmeniaca
-
the half lives of free enzyme at 25 and 50°C are 49.9 and 30.5 h, respectively and these correspondingly are 96.3 and 43.6 h for immobilized enzyme onto Eupergit C, and 138.6 and 50.2 h for immobilized enzyme onto Eupergit C 250 L
30 - 50
1 h, stable, hydroxynitrile lyase from leaves, hydroxynitrile lyase expressed in Pichia pastoris, hydroxynitrile lyase expressed in Escherichia coli, hydroxynitrile lyase mutant N105Q expressed in Pichia pastoris
4
-
the enzyme does not show any significant loss of activity at 4°C, hence it has good potential for industrial application
5 - 20
Prunus pseudoarmeniaca
-
free and immobilized enzyme preparations show optimal carboligation activity at 5°C. As the temperature is increased from 5 to 20°C, the yields decrease, however enantiomeric excess values (99%) unchange
75
1 h, complete loss of activity
90
-
1 h, 85% loss of activity
additional information
the hydroxynitrile lyase from leaves and that expressed in Pichia pastoris show much better thermostability, pH stability, and organic solvent tolerance than the deglycosylated enzyme expressed in Escherichia coli and the deglycosylated mutant N105Q expressed in Pichia pastoris
10
-
half-life time: more than 96 h
10
pH 5.0, wild-type, half-life 23.6 min, surface-modified mutant, half-life 136 min
20
-
half-life time: 80 h
20
pH 5.0, wild-type, half-life 9.6 min, surface-modified mutant, half-life 134 min
30
-
half-life time: 33 h
37
-
half-life time: 6.6 h
50
-
half-life time: 0.3 h
55
-
30 min, pH 6.0, without substrate, more than 80% of the original activity is retained
55
1 h, 10% loss of activity, hydroxynitrile lyase from leaves and hydroxynitrile lyase expressed in Pichia pastoris
60
1h, hydroxynitrile lyase from leaves loses 20% of its activity. Hydroxynitrile lyase expressed in Pichia pastoris loses 40% of its activity. Hydroxynitrile lyase expressed in Escherichia coli and hydroxynitrile lyase mutant N105Q expressed in Pichia pastoris completely lose activity
65
1 h, 10% loss of activity
65
in the pH-range 4-8 the Tm is constant at 76°C. At pH 9 and 10 the Tm values are slightly lower, 70°C and the lowest Tm of 65°C is measured at pH 3
70
1 h, 40% loss of activity
70
1 h, hydroxynitrile lyase from leaves loses 60% of its activity, Hydroxynitrile lyase expressed in Pichia pastoris, completely loses activity
70
in the pH-range 4-8 the Tm is constant at 76°C. At pH 9 and 10 the Tm values are slightly lower, 70°C and the lowest Tm of 65°C is measured at pH 3
70
Tm-value at pH 9 and 10
70
-
1 h, 30% loss of activity
70
60 min, 68% residual activity
76
in the pH-range 4-8 the Tm is constant at 76°C. At pH 9 and 10 the Tm values are slightly lower, 70°C and the lowest Tm of 65°C is measured at pH 3
76
Tm-value in the pH-range 4-8
80
-
1 h, 70% loss of activity
80
60 min, 28% residual activity