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Literature summary for 4.1.2.10 extracted from

  • Kopka, B.; Diener, M.; Wirtz, A.; Pohl, M.; Jaeger, K.E.; Krauss, U.
    Purification and simultaneous immobilization of Arabidopsis thaliana hydroxynitrile lyase using a family 2 carbohydrate-binding module (2015), Biotechnol. J., 10, 811-819 .
    View publication on PubMed
Search for more literature for 4.1.2.10

Application

Application Comment Organism
synthesis an easy one-step immobilization of a complex Arabidopsis thaliana hydroxynitrile lyase fusion protein is possible starting from an Escherichia coli crude cell extract (producing a carbohydrate-binding module-containing fusion protein) and cost efficient cellulosic carrier materials. Highly-specific site-selective immobilization of the target protein is achieved by fusing the family 2 carbohydrate-binding module of the exoglucanase/xylanase Cex from Cellulomonas fimi to the target enzyme. This yields a cheap, active, stable and recyclable immobilizate, which can be employed in micro-aqueous reaction systems to enable enantiopure cyanohydrin synthesis Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
Arabidopsis thaliana hydroxynitrile lyase fused to family 2 carbohydrate-binding module derived from the exoglucanase/xylanase Cex of Cellulomonas fimi and to a fluorescent reporter module Arabidopsis thaliana

General Stability

General Stability Organism
the fusion protein of Arabidopsis thaliana hydroxynitrile lyase and a family 2 carbohydrate-binding module derived from the exoglucanase/xylanase Cex of Cellulomonas fimi and a fluorescent reporter module protein shows increases stability at weakly acidic pH values, which is further increased by immobilization Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9LFT6
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Purification (Commentary)

Purification (Comment) Organism
the fused family 2 carbohydrate-binding module enables the purification and simultaneous immobilization of Arabidopsis thaliana hydroxynitrile lyase on various cellulosic carrier materials Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.25
-
 1.4 min (family 2 carbohydrate-binding module derived from the exoglucanase/xylanase Cex of Cellulomonas fimi fused to a fluorescent reporter module and the Arabidopsis thaliana hydroxynitrile lyase as target enzyme, immobilized on cellulosic carrier materials Avicel) Arabidopsis thaliana
4.5
-
 pH-dependent half-lives: 1.5 min (wild-type enzyme), 1.5 min (family 2 carbohydrate-binding module derived from the exoglucanase/xylanase Cex of Cellulomonas fimi fused to a fluorescent reporter module and the Arabidopsis thaliana hydroxynitrile lyase as target enzyme), 2.1 min (family 2 carbohydrate-binding module derived from the exoglucanase/xylanase Cex of Cellulomonas fimi fused to a fluorescent reporter module and the Arabidopsis thaliana hydroxynitrile lyase as target enzyme, immobilized on cellulosic carrier materials Avicel) Arabidopsis thaliana
4.75
-
 pH-dependent half-lives: 4.4 min (wild-type enzyme), 5.5 min (family 2 carbohydrate-binding module derived from the exoglucanase/xylanase Cex of Cellulomonas fimi fused to a fluorescent reporter module and the Arabidopsis thaliana hydroxynitrile lyase as target enzyme), 30.6 min (family 2 carbohydrate-binding module derived from the exoglucanase/xylanase Cex of Cellulomonas fimi fused to a fluorescent reporter module and the Arabidopsis thaliana hydroxynitrile lyase as target enzyme, immobilized on cellulosic carrier materials Avicel) Arabidopsis thaliana