4.1.2.10: (R)-mandelonitrile lyase

This is an abbreviated version!
For detailed information about (R)-mandelonitrile lyase, go to the full flat file.

Word Map on EC 4.1.2.10

Reaction

(R)-mandelonitrile
=
cyanide
+
benzaldehyde

Synonyms

Oxynitrilase, Hydroxynitrile lyase, HNL, (R)-Oxynitrilase, D-Hydroxynitrile lyase, (R)-Mandelonitrile lyase, PhaMDL, (R)-(+)-Mandelonitrile lyase, MDL, D-Oxynitrilase, Lyase, mandelonitrile, D-alpha-hydroxynitrile lyase, PaHNL, PaHNL1, FAD-HNL, hydroynitrile lyase, (R)-hydroxynitrile lyase, mandelonitrile lyase, almond oxynitrilase, R-hydroxynitrile lyase, HNL1, Mdl1, (R)-HNL, PmHNL, PaHNL5, EjHNL, R-selective hydroxynitrile lyase, (R)-PeHNL, R-selective HNL, AtHNL, PaMDL, (R)-HNL5, (R)-selective HNL, HNL2, R-HNL, cupin 2 domain-containing protein, cupin 2 conserved barrel domain protein, AciX9_0562, HNL4, HNL5, NdHNL, DtHNL1, APHNL, ParsHNL

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.2 Aldehyde-lyases
                4.1.2.10 (R)-mandelonitrile lyase

General Stability

General Stability on EC 4.1.2.10 - (R)-mandelonitrile lyase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
(R)-oxynitrilase immobilized as a cross-linked enzyme aggregate via precipitation with 1,2-dimethoxyethane and subsequent cross-linking using glutaraldehyde is stable and recyclable
-
both of immobilized enzymes (Eupergit C and Eupergit C250L) are used repeatedly 20times and the residual activities are about 97% of their initial activities
Prunus pseudoarmeniaca
-
crosslinked enzyme aggregates result in significant enhancement of the biocatalyst stability under acidic conditions (activity is retained after 168 h at pH 2.4)
-
the cross-linked enzyme aggregate of Prunus dulcis hydroxynitrile lyase can be reused several times without loss of enantioselectivity
-
the enzyme is stabilized by addition of sorbitol and saccharose
-
the enzyme is stable without the substrate but is remarkably unstable upon incubation with benzaldehyde and KCN
-
the fusion protein of Arabidopsis thaliana hydroxynitrile lyase and a family 2 carbohydrate-binding module derived from the exoglucanase/xylanase Cex of Cellulomonas fimi and a fluorescent reporter module protein shows increases stability at weakly acidic pH values, which is further increased by immobilization
the immobilized enzyme preparations (immobilized onto Eupergit CM and Eupergit C 250 L supports) are successfully reused 10times without loss of activity and enantioselectivity
-
the presence of glycan can prevent the self-degradation of this enzyme at 10°C