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4.1.2.10: (R)-mandelonitrile lyase

This is an abbreviated version!
For detailed information about (R)-mandelonitrile lyase, go to the full flat file.

Word Map on EC 4.1.2.10

Reaction

(R)-mandelonitrile
=
cyanide
+
benzaldehyde

Synonyms

(R)-(+)-Mandelonitrile lyase, (R)-HNL, (R)-HNL5, (R)-hydroxynitrile lyase, (R)-Mandelonitrile lyase, (R)-Oxynitrilase, (R)-PeHNL, (R)-selective HNL, AciX9_0562, almond oxynitrilase, APHNL, AtHNL, cupin 2 conserved barrel domain protein, cupin 2 domain-containing protein, D-alpha-hydroxynitrile lyase, D-Hydroxynitrile lyase, D-Oxynitrilase, DtHNL1, EjHNL, FAD-HNL, HNL, HNL1, HNL2, HNL4, HNL5, Hydroxynitrile lyase, hydroynitrile lyase, Lyase, mandelonitrile, mandelonitrile lyase, MDL, Mdl1, NdHNL, Oxynitrilase, PaHNL, PaHNL1, PaHNL5, PaMDL, ParsHNL, PhaMDL, PmHNL, R-HNL, R-hydroxynitrile lyase, R-selective HNL, R-selective hydroxynitrile lyase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.2 Aldehyde-lyases
                4.1.2.10 (R)-mandelonitrile lyase

Cloned

Cloned on EC 4.1.2.10 - (R)-mandelonitrile lyase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Arabidopsis thaliana hydroxynitrile lyase fused to family 2 carbohydrate-binding module derived from the exoglucanase/xylanase Cex of Cellulomonas fimi and to a fluorescent reporter module
cloned and sequenced
expresion in Pichia pastoris
expressed in Escherichia coli BL21(DE3) cells
expressed in Pichia pastoris
-
expressed in Pichia pastoris strain GS115
expression in Escherichia coli
expression in Escherichia coli. Recombinant production of DtHNL1 in Komagataella phaffii under control of the strong and tightly regulated AOX1 promoter. Two different shuttle vectors are used, pPpT4_S and pPpB1, to obtain strains with optimal gene copy numbers for efficient heterologous expression
-
expression of mutant and wild-type enzymes in Pichia pastoris X33
heterologously expression of the His-tagged hydroxynitrile lyase isoenzymes (HNL1, HNL2, HNL4 and HNL5) in Pichia pastoris
-
mutant V317A of hydroxynitrile lyase isoenzyme 5 is expressed in Aspergillus niger
overexpressed in Pichia pastoris
-
the enzyme is cloned and expressed in Escherichia coli BL21(DE3) and Pichia pastoris GS115 cells without a signal peptide sequence
the highly glycosylated Prunus amygdalus HNL isoenzyme 5 (PaHNL5 V317A) is expressed in Aspergillus niger. The recombinant protein is highly glycosylated as confirmed by SDS-page and mass spectrometry