4.1.2.10: (R)-mandelonitrile lyase

This is an abbreviated version!
For detailed information about (R)-mandelonitrile lyase, go to the full flat file.

Word Map on EC 4.1.2.10

Reaction

(R)-mandelonitrile
=
cyanide
+
benzaldehyde

Synonyms

Oxynitrilase, Hydroxynitrile lyase, HNL, (R)-Oxynitrilase, D-Hydroxynitrile lyase, (R)-Mandelonitrile lyase, PhaMDL, (R)-(+)-Mandelonitrile lyase, MDL, D-Oxynitrilase, Lyase, mandelonitrile, D-alpha-hydroxynitrile lyase, PaHNL, PaHNL1, FAD-HNL, hydroynitrile lyase, (R)-hydroxynitrile lyase, mandelonitrile lyase, almond oxynitrilase, R-hydroxynitrile lyase, HNL1, Mdl1, (R)-HNL, PmHNL, PaHNL5, EjHNL, R-selective hydroxynitrile lyase, (R)-PeHNL, R-selective HNL, AtHNL, PaMDL, (R)-HNL5, (R)-selective HNL, HNL2, R-HNL, cupin 2 domain-containing protein, cupin 2 conserved barrel domain protein, AciX9_0562, HNL4, HNL5, NdHNL, DtHNL1, APHNL, ParsHNL

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.2 Aldehyde-lyases
                4.1.2.10 (R)-mandelonitrile lyase

pH Stability

pH Stability on EC 4.1.2.10 - (R)-mandelonitrile lyase

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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.6
1 h, about % loss of activity
749169
3 - 9
60 min, stable
691375
3.5 - 10
activities of all the hydroxynitrile lyase are broadly stable over the range from pH 3.5 to 10.0, although the activity of hydroxynitrile lyase expressed in Escherichia coli is reduced to 60% and 70% at pH 7.0 and 10.0, respectively
747548
3.5 - 8
-
30°C, 30 min, stable
747326
4.5
-
1 h, complete loss of activity
747948
4.75
wild-type, half-life 2.2 min, flavin-based fluorescent reporter fusion protein half-life 55-95 min
726741
5.4
-
half-life time: 2 h, the deactivation of the enzyme at slightly acidic pH is a result of pronounced structural unfolding
704762
5.5
1 h, about 20% loss of activity
749169
6
20°C, wild-type, half-life 60 h, surface-modified mutant, half-life 52.2 h
727311
6 - 12
-
1 h, enzyme is stable
747948
8 - 10
1 h, no loss of activity
749169