3.1.3.46: fructose-2,6-bisphosphate 2-phosphatase

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For detailed information about fructose-2,6-bisphosphate 2-phosphatase, go to the full flat file.

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Synonyms

6-phosphofructo-2-kinase/2,6-bisphosphatase 3, 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, 6-phosphofructo-2-kinase/fructose-2 6-biphosphatase, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphase, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (gene PFKFB3), 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-4, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphate, 6PF2K/Fru-2,6-P2ase, D-fructose-2,6-bisphosphatase, F-2,6-P2, F26BPase, F2KP, FBPase, FBPase-2, fructose-2,6-bisphosphatase, fructose-2,6-diphosphatase, heart 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase, Lm1, More, OsF2KP1, OsF2KP2, Pfk-2, PFK-2/FBPase, PFK-2/FBPase-2, PFK-2/FDPase-2, PFK-2/Fru-2,6-P2, PFK2, PFK2/FBPase2, Pfk26, Pfk27, PFKFB, PFKFB-3, PFKFB-4, PFKFB1, PFKFB2, PFKFB3, PFKFB4, phosphatase, fructose 2,6-di-, RH2K, TIGAR

ECTree

     3 Hydrolases

         3.1 Acting on ester bonds

             3.1.3 Phosphoric-monoester hydrolases

                3.1.3.46 fructose-2,6-bisphosphate 2-phosphatase

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Results	in table
57	Activating Compound
212	AA Sequence
13	Application
1	CAS Registry Number
43	Cloned(Commentary)
10	Crystallization (Commentary)
2102	Disease/ Diagnostics
5	Expression
14	General Information
22	IC50 Value
111	Inhibitors
7	Ki Value [mM]
29	KM Value [mM]
7	Localization
5	Metals/Ions
29	Molecular Weight [Da]
96	Natural Substrates/ Products (Substrates)
299	Organism
3	Pathway
41	PDB ID
12	pH Optimum
1	pH Range
1	pI Value
12	Posttranslational Modification
22	Protein Variants
21	Purification (Commentary)
55	Reaction
15	Reaction Type
123	Reference
205	Source Tissue
7	Specific Activity [micromol/min/mg]
7	Storage Stability
163	Substrates and Products (Substrate)
51	Subunits
240	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Stability [°C]
2	Turnover Number [1/s]

General Information

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General Information on EC 3.1.3.46 - fructose-2,6-bisphosphate 2-phosphatase

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GENERAL INFORMATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

malfunction

Homo sapiens

Q16877

knockdown of isoform PFKFB4 reduces tumor growth, glucose uptake and beta-D-fructose 2,6-bisphosphate and increases apoptosis

730530

metabolism

2 entries

physiological function

9 entries

additional information

2 entries

metabolism

Rattus norvegicus

-

analysis of glucokinase/6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase complex formation, binding and activation of GK by PFK-2/FBPase-2 in beta-cells is promoted by glucose, resulting in an enhancement of insulin secretion at stimulatory glucose concentrations, without affecting basal insulin secretion

716226

metabolism

Mus musculus

-

flux through phosphofructokinase-1 is controlled by the bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 via production/degradation of fructose-2,6-bisphosphate, a potent allosteric activator of phosphofructokinase-1, as well as direct activation of glucokinase due to a protein-protein interaction

730691

physiological function

Homo sapiens

-

PFKFB2 has a critical role in glucose uptake and glucose-dependent lipid synthesis. Induction of de novo lipid synthesis by androgen requires the transcriptional up-regulation of HK2 and PFKFB2, and phosphorylation of PFKFB2 generated by the PI3K/Akt signalling pathway to supply the source for lipogenesis from glucose in prostate cancer cells

714090

physiological function

Rattus norvegicus

-

the bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase acts as an endogenous glucokinase activator

716226

physiological function

Homo sapiens

Q16877

fructose-2,6-bisphosphate synthesis by 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 is required for the glycolytic response to hypoxia and tumor growth

730530

physiological function

Mus musculus

-

PFK2/FBPase2 is most helpful to the glucose-sensing capacity of glucokinase in a fasted organism, or in the transition to threshold as glucose is elevated during feeding

730691

physiological function

Homo sapiens

Q16875

both a PFKFB3 inhibitor or PFKFB3 silencing by siRNA suppress the basal and the H2O2-induced autophagy concomitantly with the inhibition of AMPK activity. Overexpression of wild-type PFKFB3 promotes H2O2-induced autophagy, but mutant K472/473A, which lost nuclear localizing property, inhibits the autophagic process. The K472/473A mutant stimulates more lactate production, and decreases the activity of AMPK compared to the wild-type

751774

physiological function

Homo sapiens

Q16875

overexpression of microRNA miR-26b represses PFKFB3 mRNA and protein levels followed by modulation of the expression of glycolytic components such as LDHA, GLUT-1 and markers of invasion and cell cycle such as MMP-9, MMP-2, cyclin D1 and p27. The binding site for miR-26b is predicted in the 3'-untranslated region of the PFKFB3 gene

751765

physiological function

Mus musculus

Q8BZA9

TIGAR potently inhibits NF-kappaB-dependent gene expression by suppressing the upstream activation of IKKbeta phosphorylation and kinase activation. Inhibition occurs through a direct binding competition between NEMO and TIGAR for association with the linear ubiquitination assembly complex LUBAC. A TIGAR phosphatase activity-deficient mutant is equally effective as wild-type TIGAR in inhibiting NEMO linear ubiquitination. TIGAR knockout mice display enhanced adipose tissue NF-kappaB signaling, and adipocyte-specific overexpression of TIGAR suppresses adipose tissue NF-kappaB signaling

751121

physiological function

Homo sapiens

Q16875

Transforming growth factor TGFbeta1 induces isoform PFKFB3 expression and stimulates glycolysis in Panc1 cells. siRNA silencing of PFKFB3 prevents the stimulation of glycolysis and in vitro invasion ability of Panc1 cells by TGFbeta1. PFKFB3 silencing suppresses the TGFbeta1-mediated induction of the Snail protein

749813

physiological function

Homo sapiens

Q16877

tumor suppressor p53 regulates the expression of PFKFB4 and p53-deficient cancer cells are highly dependent on the function of the enzyme. Depletion of PFKFB4 from p53-deficient cancer cells increases levels of fructose-2,6-bisphosphate, leading to increased glycolytic activity but decreased routing of metabolites through the oxidative arm of the pentose-phosphate pathway. PFKFB4 is also required to support the synthesis and regeneration of nicotinamide adenine dinucleotide phosphate (NADPH) in p53-deficient cancer cells. Depletion of PFKFB4-attenuates cellular biosynthetic activity and results in the accumulation of reactive oxygen species and cell death in the absence of p53. Silencing of PFKFB4-induces apoptosis in p53-deficient cancer cells in vivo and interferes with tumor growth

751761

additional information

Homo sapiens

-

androgen stimulates glycolysis for de novo lipid synthesis by increasing the activities of hexokinase 2 and 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2, up-regulation of PFKFB2 expression is mediated by the direct binding of ligand-activated androgen receptor to the PFKFB2 promoter

714090

additional information

Homo sapiens

-

bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase

713740