3.1.3.46: fructose-2,6-bisphosphate 2-phosphatase

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Word Map on EC 3.1.3.46

3.1.3.46

fructose-1,6-bisphosphatase

gluconeogenesis

gluconeogenic

amp

6-phosphate

1,6-bisphosphatase

tp53-induced

phosphoenolpyruvate

isozyme

chloroplast

endothelial

glycogen

pentose

6-phosphofructo-1-kinase

hepatocytes

carboxykinase

fructose-6-phosphate

hypoxia-inducible

glucokinase

camp-dependent

warburg

pepck

3.1.3.11

glucose-induced

g6pase

spinach

hk2

fructose-1

hypoglycemia

glucose-6-phosphatase

hexokinase

fru-6-p

glycogenolysis

calvin

p53-inducible

anaphase-promoting

phosphofructokinase-1

phosphoenzyme

fructose-6-p

molecular biology

medicine

pharmacology

agriculture

pentose-phosphate

z-line

Reaction

Synonyms

6-phosphofructo-2-kinase/2,6-bisphosphatase 3, 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, 6-phosphofructo-2-kinase/fructose-2 6-biphosphatase, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphase, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (gene PFKFB3), 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-4, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphate, 6PF2K/Fru-2,6-P2ase, D-fructose-2,6-bisphosphatase, F-2,6-P2, F26BPase, F2KP, FBPase, FBPase-2, fructose-2,6-bisphosphatase, fructose-2,6-diphosphatase, heart 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase, Lm1, More, OsF2KP1, OsF2KP2, Pfk-2, PFK-2/FBPase, PFK-2/FBPase-2, PFK-2/FDPase-2, PFK-2/Fru-2,6-P2, PFK2, PFK2/FBPase2, Pfk26, Pfk27, PFKFB, PFKFB-3, PFKFB-4, PFKFB1, PFKFB2, PFKFB3, PFKFB4, phosphatase, fructose 2,6-di-, RH2K, TIGAR

ECTree

3 Hydrolases

3.1 Acting on ester bonds

3.1.3 Phosphoric-monoester hydrolases

3.1.3.46 fructose-2,6-bisphosphate 2-phosphatase

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Results	in table
57	Activating Compound
212	AA Sequence
13	Application
1	CAS Registry Number
43	Cloned(Commentary)
10	Crystallization (Commentary)
2102	Disease/ Diagnostics
5	Expression
14	General Information
22	IC50 Value
111	Inhibitors
7	Ki Value [mM]
29	KM Value [mM]
7	Localization
5	Metals/Ions
29	Molecular Weight [Da]
96	Natural Substrates/ Products (Substrates)
299	Organism
3	Pathway
41	PDB ID
12	pH Optimum
1	pH Range
1	pI Value
12	Posttranslational Modification
22	Protein Variants
21	Purification (Commentary)
55	Reaction
15	Reaction Type
123	Reference
205	Source Tissue
7	Specific Activity [micromol/min/mg]
7	Storage Stability
163	Substrates and Products (Substrate)
51	Subunits
240	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Stability [°C]
2	Turnover Number [1/s]

Purification

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Purification on EC 3.1.3.46 - fructose-2,6-bisphosphate 2-phosphatase

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10000fold

15fold

300fold

Saccharomyces cerevisiae

94928

5600fold

Pelophylax lessonae

94952

bifunctional enzyme, copurification with phosphofructokinase 2

2 entries

by Cibacron Blue-Sepharose and Sephadex G-50 column chromatography

2 entries

by Sephadex G-25 column chromatography

Arabidopsis thaliana

675637

copurification with phosphofructokinase 2

Rattus norvegicus

94945

glutathione Sepharose 4 column chromatography

Homo sapiens

729389

Ni-NTA affinity column chromatography

partially from liver

recombinant His-tagged inducible isozyme PFKFB3 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, after which the tag is cleaved off by thrombin, followed by anion exchange chromatography, to homogeneity

Homo sapiens

P16118

665727

recombinant His-tagged isozymes 1-4 from purified trypomastigotes, by anion exchange and adsorption chromatography, ultrafiltration and dialysis

Trypanosoma brucei

661698

recombinant His6-tagged bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase from Escherichia coli strain BL21(DE3) pLysS by nickel affinity chromatography and anion exchange chromatography with triethylammonium bicarbonate as eluant

two isoenzymes

two isoenzymes, partially purified

Spinacia oleracea

94929

using Ni-NTA affinity columns

Homo sapiens

681437