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3.1.1.32: phospholipase A1

This is an abbreviated version!
For detailed information about phospholipase A1, go to the full flat file.

Word Map on EC 3.1.1.32

Reaction

phosphatidylcholine
+
H2O
=
2-acylglycerophosphocholine
 +
a carboxylate

Synonyms

Allergen Dol m 1.01, Allergen Dol m 1.02, AtLCAT3, BveA, DAD1, DDHD1, DDL1, endothelial lipase, H-Rev107, hepatic lipase, intracellular phospholipase A1, IPLA-1, lacrimal lipase, lipase H, LipH, LIPI, LPC, membrane-associated phosphatidic acid-selective phospholipase A1, More, mPA-PLA1, mPA-PLA1alpha, mPA-PLA1beta, OM PLA, OMPLA, outer membrane phospholipase A, PA-PLA1, PA-PLA1/DDHD1, pancreatic lipase-associated protein 2, pancreatic lipase-related protein 2, PAPLA1, phosphatidate 1-acylhydorolase, phosphatidic acid phospholipase A1, phosphatidic acid-preferring phospholipase A1, phosphatidic acid-preferring phospholipase A1/DDHD1, phosphatidic acid-selective phospholipase A, phosphatidic acid-specific phospholipase A1, Phosphatidylcholine 1-acylhydrolase, phospholipase A1, phospholipase A1 alpha, phospholipase A1/2, phospholipase A1beta, phospholipase A1gamma, phospholipase beta, Pla, PLA 1, PLA A1, PLA1, PLA1/2, PLA1A, PLA1gamma, PLA1S, Plase A1, PLRP2, PPL, Rab5-activated phospholipase A1, RP534 PLA, RP534 protein, Ves a 1, Ves v 1, vespapase, VesTS1, VipD, Yor022c

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.32 phospholipase A1

Cloned

Cloned on EC 3.1.1.32 - phospholipase A1

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
4 isozymes Ves a 1.01-1.04, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, isozyme peptide mapping and sequence comparisons
-
cDNA encoding C-terminally FLAG-tagged H-Rev107. Subcloned into the HindIII and EcoRI sites of pcDNA3.1 (+). Expression vector harboring H-Rev107 cDNA introduced into COS-7 cells
cloning of cDNA, transient expression in 293 T cells
-
DNA and amino acid equence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) pLysS, the enzyme is mainly localized in inclusion bodies
DNA and amino acid sequence analysis
-
DNA and amino acid sequence analysis of isozymes, isozyme genetic structures, location of isozyme PS-PLA1 or PLA1A encoding gene on chromosome 3q13.2-q13.3, phylogenetic analysis of isozymes, overview
-
DNA and amino acid sequence analysis of isozymes, location of isozyme PS-PLA1 or PLA1A encoding gene on chromosome 16
-
expressed in Arabidopsis thaliana
expressed in CHO cells
-
expressed in HEK-293 cells
-
expressed in Pichia pastoris
expression HeLa, CHO-K1 and Sf9 cells
expression in Escherichia coli
expression in Escherichia coli and cell free system
-
expression in Saccharomyces cerevisiae and Aspergillus oryzae
-
expression in Sf9 insect cells
expression of FLAG-tagged wild-type and mutant enzymes in HeLa cells and in siRNA-treated knockout mutant HeLa cells. Upon overexpression of wild-type enzyme, the tubular structures become fragmented, expression of the mutant S537A has no effect
-
expression of IPLA-1 in HEK-293 cells
-
expression of N156A mutant
-
expression of wild-type and PCR evolved phospholipase A1 variants in Escherichia coli
expression vector harboring H-Rev107 with a FLAG tag at the N terminus. Expression vector harboring H-Rev107 cDNA introduced into COS-7 cells
expresssion of His-tagged wild-type and mutant enzymes in Escherichia coli strain C41[DE3]
-
gene CG8552, expression of a 31-kb genomic enzyme fragment modified to carry a V5 epitope at the N-terminus, i.e. V5-N-PAPLA1, the recombinant protein is transiently expressed and fully functional in the Drosophila S2 cell line and rescues all the phenotype of a null mutant
gene pla, DNA and amino acid sequence determination and analysis, expression and extracellular production of the recombinant enzyme in Streptomyces lividans from expression vector pUC702/pla
genes Vesv1 and Vesv5, subcloning in Escherichia coli strain Dh5alpha, recombinant expression of His-tagged Vesv5 and Vesv1 wild-type and His-tagged Vesv1 mutant enzymes in Pichia pastoris strains X33 and KM71H as secreted proteins, method optimization, overview
-
LIPH and LIPI, DNA and amino acid sequence determination and analysis, conserved genomic organization of LIPI and LIPH, the LIPI locus is neighbored by a member of the RNA binding motif, RBM, family, RBM11
-
PCR product subcloned into the pAcGP67-B baculovirus transfer vector, expressed as wild-type and enzymatically inactivated mutant in Sf9-insect cells
-
phylogenetic tree, overview
polypeptide (amino acid number 368-486 of intracellular PLA1gamma) expressed as a glutathione S-transferase fusion protein in Escherichia coli. Purified protein used to immunize rats. The cDNA encoding intracellular PLA1gamma cloned into pEGFP-C3 vector and stably expressed in CHO cells
-
recombinant enzyme expression
recombinant expression in COS-1 cells, recombinant expression in Saccharomyces cerevisiae strain INVSc1, Rab5 enhances the growth defect of yeast cells producing VipD
-