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3.1.1.32: phospholipase A1

This is an abbreviated version!
For detailed information about phospholipase A1, go to the full flat file.

Word Map on EC 3.1.1.32

Reaction

phosphatidylcholine
+
H2O
=
2-acylglycerophosphocholine
 +
a carboxylate

Synonyms

Allergen Dol m 1.01, Allergen Dol m 1.02, AtLCAT3, BveA, DAD1, DDHD1, DDL1, endothelial lipase, H-Rev107, hepatic lipase, intracellular phospholipase A1, IPLA-1, lacrimal lipase, lipase H, LipH, LIPI, LPC, membrane-associated phosphatidic acid-selective phospholipase A1, More, mPA-PLA1, mPA-PLA1alpha, mPA-PLA1beta, OM PLA, OMPLA, outer membrane phospholipase A, PA-PLA1, PA-PLA1/DDHD1, pancreatic lipase-associated protein 2, pancreatic lipase-related protein 2, PAPLA1, phosphatidate 1-acylhydorolase, phosphatidic acid phospholipase A1, phosphatidic acid-preferring phospholipase A1, phosphatidic acid-preferring phospholipase A1/DDHD1, phosphatidic acid-selective phospholipase A, phosphatidic acid-specific phospholipase A1, Phosphatidylcholine 1-acylhydrolase, phospholipase A1, phospholipase A1 alpha, phospholipase A1/2, phospholipase A1beta, phospholipase A1gamma, phospholipase beta, Pla, PLA 1, PLA A1, PLA1, PLA1/2, PLA1A, PLA1gamma, PLA1S, Plase A1, PLRP2, PPL, Rab5-activated phospholipase A1, RP534 PLA, RP534 protein, Ves a 1, Ves v 1, vespapase, VesTS1, VipD, Yor022c

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.32 phospholipase A1

Inhibitors

Inhibitors on EC 3.1.1.32 - phospholipase A1

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
Al3+
Corticium centrifugum
-
inhibition of phospholipase A1 activity and lysophospholipase activity
arachidonyl trifluoromethyl ketone analogue
-
-
Cationic amphiphilic drugs
-
-
-
Cd2+
complete inhibition at 2 mM
CHAPS
-
-
chlorpromazine
-
1 mM, 50% inhibition
choline plasmalogen
-
inhibits hydrolysis of phosphatidylethanolamine
Co2+
43% inhibition at 2 mM
cocaine
-
10 mM, below 10% residual activity
Cr3+
51.4% relative activity at 5 mM
DFP
-
-
di-isopropyl fluorophosphate
TbPLA1 possesses an active-site serine residue, but is relatively insensitive to serine-modifying reagents such as di-isopropyl fluorophosphate and PMSF
diethyl-p-nitrophenyl phosphate
-
diisopropyl fluorophosphate
-
IC50: 8 mM
dithiothreitol
-
1 mM, complete inhibition
DNA containing thymine glycol
-
ethanolamine plasmalogen
-
inhibits hydrolysis of phosphatidylethanolamine
gentamycin
-
the inhibition by aminoglycosides appears to be due to substrate depletion caused by binding of aminoglycosides to the negatively charged phospholipid and can be overcome by raising the substrate concentration
GSK264220A
-
-
guanosine 5'-[gamma-thio]triphosphate
-
-
Insulin
-
significantly reduces endothelial lipase mRNA and protein expression in a dose dependent manner
-
iodoacetic acid
KCl
-
2 M, inactivation of the soluble enzyme, no inactivation of the membrane-bound enzyme
lauryldimethylbenzyl ammonium chloride
-
-
liponox DCH
-
1%, i.e. polyoxyethylene alkylether
methyl arachidonyl fluorophosphonate
-
blocks phospholipase A1 as well as phospholipase A2 activity
Mg2+
13.5% relative activity at 5 mM
MgCl2
-
slight
N-bromosuccinimide
Corticium centrifugum
-
-
Na+
54.7% relative activity at 5 mM
Ni2+
57% inhibition at 2 mM
octyl-beta-glucoside
-
-
p-Diazobenzenesulfonic acid
Corticium centrifugum
-
-
palmitoyl trifluoromethyl ketone analogue
-
-
rabbit anti-phospholipase A antibodies
-
-
-
Rosenthal's inhibitor
-
-
sodium deoxycholate
-
-
Sodium vanadate
0.01 mM, 50% inhibition
taurodeoxycholate
-
-
tetrahydrolipstatin
-
-
thiol reagents
-
protected by 2-mercaptoethanol
tobramycin
-
the inhibition by aminoglycosides appears to be due to substrate depletion caused by binding of aminoglycosides to the negatively charged phospholipid and can be overcome by raising the substrate concentration
Triton X-100
additional information
-