3.1.1.32: phospholipase A1

This is an abbreviated version!
For detailed information about phospholipase A1, go to the full flat file.

Reaction

Synonyms

Allergen Dol m 1.01, Allergen Dol m 1.02, AtLCAT3, BveA, DAD1, DDHD1, DDL1, endothelial lipase, H-Rev107, hepatic lipase, intracellular phospholipase A1, IPLA-1, lacrimal lipase, lipase H, LipH, LIPI, LPC, membrane-associated phosphatidic acid-selective phospholipase A1, More, mPA-PLA1, mPA-PLA1alpha, mPA-PLA1beta, OM PLA, OMPLA, outer membrane phospholipase A, PA-PLA1, PA-PLA1/DDHD1, pancreatic lipase-associated protein 2, pancreatic lipase-related protein 2, PAPLA1, phosphatidate 1-acylhydorolase, phosphatidic acid phospholipase A1, phosphatidic acid-preferring phospholipase A1, phosphatidic acid-preferring phospholipase A1/DDHD1, phosphatidic acid-selective phospholipase A, phosphatidic acid-specific phospholipase A1, Phosphatidylcholine 1-acylhydrolase, phospholipase A1, phospholipase A1 alpha, phospholipase A1/2, phospholipase A1beta, phospholipase A1gamma, phospholipase beta, Pla, PLA 1, PLA A1, PLA1, PLA1/2, PLA1A, PLA1gamma, PLA1S, Plase A1, PLRP2, PPL, Rab5-activated phospholipase A1, RP534 PLA, RP534 protein, Ves a 1, Ves v 1, vespapase, VesTS1, VipD, Yor022c

ECTree

     3 Hydrolases

         3.1 Acting on ester bonds

             3.1.1 Carboxylic-ester hydrolases

                3.1.1.32 phospholipase A1

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Results	in table
24	Activating Compound
10146	AA Sequence
20	Application
1	CAS Registry Number
40	Cloned(Commentary)
6	Crystallization (Commentary)
441	Disease/ Diagnostics
3	Expression
32	General Information
4	General Stability
1	IC50 Value
101	Inhibitors
3	kcat/KM [mM/s]
14	KM Value [mM]
81	Localization
35	Metals/Ions
40	Molecular Weight [Da]
44	Natural Substrates/ Products (Substrates)
7	Organic Solvent Stability
314	Organism
7	Pathway
17	PDB ID
41	pH Optimum
12	pH Range
5	pH Stability
6	pI Value
5	Posttranslational Modification
60	Protein Variants
51	Purification (Commentary)
8	Reaction
1	Reaction Type
141	Reference
161	Source Tissue
34	Specific Activity [micromol/min/mg]
6	Storage Stability
264	Substrates and Products (Substrate)
34	Subunits
155	Synonyms
1	Systematic Name
21	Temperature Optimum [°C]
8	Temperature Range [°C]
16	Temperature Stability [°C]
5	Turnover Number [1/s]

Application

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Application on EC 3.1.1.32 - phospholipase A1

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APPLICATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

analysis

Aspergillus oryzae

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development of a spectrophotometric assay to measure, continuously and specifically, phospholipase A1 or phospholipase A2 activities using synthetic glycerophosphatidylcholines containing alpha-oleostearic acid. Substrates 1-alpha-eleostearoyl-2-octadecyl-rac-glycero-3-phosphocholine or 1-octadecyl-2-alpha-eleostearoyl-rac-glycero-3-phosphocholine differentiate, with excellent accuracy, between PLA1 and PLA2 activity

751273

degradation

Thermomyces lanuginosus

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hydrolysis of nonpolar lipids, i.e. triacylglycerols, diacylglycerols and monoacylglycerols, when crude sunflower lecithin is treated with commercial product Lecitase VR Ultra. During the reaction, an acyl-migration phenomenon is observed. In 1 h of reaction the content of triacylglycerols decreases to 54%, while diacylglycerol and monoacylglycerol concentrations increase from 0.4 to 3.5 and from 1.9 to 6.5 g/100 g of crude lecithin, respectively. Along the reaction, different contents of glycerides can be achieved

749788

food industry

Fusarium venenatum

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the enzyme improves foaming stability and properties of skim milk and whey, implying that phospholipases can be useful tools for modifying the functionality of dairy products and ingredients

680300

industry

Serratia sp. MK1

-

enzyme can be used for degumming crude plant oil

750141

medicine

4 entries

nutrition

Fusarium venenatum

-

the enzyme improves foaming stability and properties of skim milk and whey, implying that phospholipases can be useful tools for modifying the functionality of dairy products and ingredients

680300

pharmacology

Vespula vulgaris

-

the recombinant protein is allergenic in a biological assay as demonstrated by its capability to induce histamine release of wasp venom-sensitive basophils. The recombinant phospholipase A1 presents a good candidate for wasp venom immunotherapy

716710

synthesis

10 entries

medicine

Rattus norvegicus

-

acidotropic accumulation of cocaine in lysosomes inhibits acid phospholipase A1 and inactivates acid sphingomyelinase, inducing a mixed lysosomal lipidosis

666965

medicine

Vespula vulgaris

-

may provide a valuable tool for diagnostic and therapeutic approaches in hymenoptera venom allergy. Recombinant Ves v 1 is an essential component to assess the sensitisation of individuals to yellow jacket venom and its recombinant availability complemented with Ves v 5 and phospholipase A2 from honeybee venom (Api m 1) allows for clear assignment of sensitisation patterns

708202

medicine

Homo sapiens

Q8NEL9

homozygous mutation c.395dup/p.Gly133Argfs*26 in DDHD1 results in a complex form of hereditary spastic paraplegia associated with retinal dystrophy and a pattern of neurodegeneration with brain iron accumulation

750476

medicine

Homo sapiens

Q53H76

role of enzyme in oncogenesis and metastasis of colorectal cancer. Tumor depth and hematogenous metastasis independently positively correlate with phospholipase A1 expression. High expression is associated with shorter disease-free survival, although it is not an independent predictive factor

749586

synthesis

Aspergillus oryzae

-

PLA1 may be used in lysolecithin production

650631

synthesis

Fusarium oxysporum

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the enzyme mixture from Thermomyces lanuginosus/Fusarium oxysporum acts as biocatalyst in a solvent-free system containing PLA1 for the modification of phosphatidylcholine from fatty acid saponification of fish oil, overview

679553

synthesis

Thermomyces lanuginosus

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the enzyme mixture from Thermomyces lanuginosus/Fusarium oxysporum acts as biocatalyst in a solvent-free system containing PLA1 for the modification of phosphatidylcholine from fatty acid saponification of fish oil, overview

679553

synthesis

Thermomyces lanuginosus

-

bio-imprinting of phospholipase A1 with 5% soybean lecithin at pH 5.0, increases its phospholipase activity in lecithin-hexane solution by 30.9fold and substantially enhances its substrate specificity. Incorporated of 105 mg diatomite/mg as an immobilization carrier further increases phospholipase activity from 562 U/g to1288 U/g

751341

synthesis

Serratia sp. MK1

-

expression of phospholipase A1 in Escherichia coli results in extremely low productivity associated with inhibition of transformed cell growth. Poduction of Serratia sp. phospholipase A1 in a cell-free protein synthesis system results in an over 1000fold higher titer of functional phospholipase A1

750141

synthesis

Aspergillus oryzae

-

immobilized Aspergillus oryzae overexpressing phospholipase A1 and cultivated in the presence of reticulated polyurethane foams, shows extracellular phospholipase A1 activities of 51.2-62.0 U/ml after 96-120 h. The extracellular phospholipase A1 activity of the immobilized cells at 0.5% polypeptone concentration is 34.8 U/ml. High growth rates of immobilized cells contribute to the enhanced phospholipase A1 production

749838

synthesis

Bacillus inaquosorum

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maximum phospholipase A1 production of 51.55 U/ml can be achieved using defatted rice bran in solid state fermentation with moisture content of 1:1.5 and after 48h of incubation at 37°C

751408

synthesis

Thermomyces lanuginosus

-

optimal reaction conditions migration for partial hydrolysis of soy phosphatidylcholine in hexane in order to maximize the lysophosphatidylcholine content while suppressing acyl are 60°C, 3 h reaction time, with a water content of 10% of phosphatidylcholine, and enzyme loading of 1% of phosphatidylcholine. Under these conditions, the reaction products contain 83.7 mol% lysophosphatidylcholine and are free of glycerylphosphorylcholine. Gycerylphosphorylcholine has a higher total unsaturated fatty acid content than original phosphatidylcholine has and is mainly composed of linoleic acid

750165

synthesis

Thermomyces lanuginosus

-

preparation of L-alpha-glycerylphosphorylcholine via phospholipase A1-catalyzed hydrolysis of soy phosphatidylcholine. Optimal conditions are temperature 50°C, reaction time 30 h, water content 69 g/100 g of phosphatidylcholine weight, and enzyme loading 13 g/100 g of phosphatidylcholine weight. The optimal n-hexane-to-water ratio in the medium is 5.8:1 (v/v). L-alpha-glycerylphosphorylcholine with purity of 99.3 g/100 g is obtained

750579

synthesis

Aspergillus oryzae NiaD300

-

immobilized Aspergillus oryzae overexpressing phospholipase A1 and cultivated in the presence of reticulated polyurethane foams, shows extracellular phospholipase A1 activities of 51.2-62.0 U/ml after 96-120 h. The extracellular phospholipase A1 activity of the immobilized cells at 0.5% polypeptone concentration is 34.8 U/ml. High growth rates of immobilized cells contribute to the enhanced phospholipase A1 production

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