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D384A
less than 10% of wild-type activity
H409L
less than 10% of wild-type activity
S177A
less than 10% of wild-type activity
T352A
about 70% of wild-type activity
Y346F
about 30% of wild-type activity
D148N
-
100% of wild-type activity
D149A
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100% of wild-type activity
D149N
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approx. 85% of wild-type activity
D184A
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approx. 30% of wild-type activity
H26C
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introduced cysteine covalently links 2 OMPLA monomers to an active dimer via disulfide bond
S152N
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approx. 6% of wild-type activity
N336Q
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the mutant shows a significant phospholipase A1 activity on mixed phospholipid-bile salt micelles
S149A
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catalytically inactive
S537A
-
site-directed mutagenesis, inactive mutant
D286A
-
site-directed mutagenesis, the active site mutant shows highly reduced activity compared to the wild-type enzyme VipD
G288A
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site-directed mutagenesis, the active site mutant shows highly reduced activity compared to the wild-type enzyme VipD
G43S
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site-directed mutagenesis, the active site mutant shows highly reduced activity compared to the wild-type enzyme VipD
G75A
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site-directed mutagenesis, the active site mutant shows highly reduced activity compared to the wild-type enzyme VipD
S73A
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site-directed mutagenesis, the active site mutant shows highly reduced activity compared to the wild-type enzyme VipD
D264A
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site-directed mutagenesis, substrate specificity compared to the wild-type enzyme, overview
S100A
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site-directed mutagenesis, substrate specificity compared to the wild-type enzyme, overview
H218A
site-directed mutagenesis, inactive mutant
H218R
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
S11A
site-directed mutagenesis, inactive mutant
S11D
site-directed mutagenesis, inactive mutant
S11E
site-directed mutagenesis, inactive mutant
S11T
site-directed mutagenesis, inactive mutant
S11Y
site-directed mutagenesis, inactive mutant
S216A
site-directed mutagenesis, inactive mutant
S216D
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
S216E
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
S216T
site-directed mutagenesis, the mutant shows 80-90% reduced activity compared to the wild-type enzyme
S216Y
site-directed mutagenesis, the mutant shows 80-90% reduced activity compared to the wild-type enzyme
H218A
-
site-directed mutagenesis, inactive mutant
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S11A
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site-directed mutagenesis, inactive mutant
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S11D
-
site-directed mutagenesis, inactive mutant
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S11E
-
site-directed mutagenesis, inactive mutant
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S216A
-
site-directed mutagenesis, inactive mutant
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V260A
-
site-directed mutagenesis, the mutation affects the enzyme's substrate specificity
D165A
-
site-directed mutagenesis of gene Vesv1
H229A
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site-directed mutagenesis of gene Vesv1, inactive mutant, that binds IgE antibodies from wasp venom allergic patients and inhibits the binding of wasp venom to IgE antibodies specific for phospholipase A1. The recombinant mutant protein is allergenic in a biological assay as demonstrated by its capability to induce histamine release of wasp venom-sensitive basophils
S137A
-
site-directed mutagenesis of gene Vesv1
S137G/N165A
-
lacks phospholipase activity
S501A
-
loss of activity
S501A
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loss of activity
-
additional information
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acl-8 acl-9 acl-10 triple mutations cause misorientation of seam cell divisions similar to that observed in ipla-1 mutants
additional information
construction of a gene CG8552 null mutant
additional information
construction of a gene CG8552 null mutant
additional information
construction of a gene CG8552 null mutant
additional information
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enzyme immobilization using Lewatit VP OC 1600 as immobilization carrier, method optimization, n-3 polyunsaturated fatty acids-enriched phosphatidylcholine is successfully produced with fatty acid from fish oil and phosphatidylcholine from soybean by immobilized phospholipase A1-catalyzed acidolysis, best at ratio of fatty acid to phophatidylcholine of 8:1 mol/mol, overview. The yield of phosphatidylcholine increases significantly by application of vacuum, even though a slight decrease of n-3 polyunsaturated fatty acid incorporation is observed
additional information
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gene lpd encoding isozyme mPA-PLA1beta, i.e. the lipid defect locus lipase LPDL, homozygous insertion mutation leads to high levels of triacylglycerols in the plasma and hepatic steatosis
additional information
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construction of enzyme knockout mutant HeLa cells by treatment with specific siRNA, phenotype, overview
additional information
homozygous mutation c.395dup/p.Gly133Argfs*26 in DDHD1 results in a complex form of hereditary spastic paraplegia associated with retinal dystrophy and a pattern of neurodegeneration with brain iron accumulation
additional information
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truncated enzyme mutant VipD2-621 is inactive with a primarily cytosolic localization, the loss of enzyme activity is likely not because of a folding defect of the VipD2621 point mutants because they still interact with Rab5c(Q80L)
additional information
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generation of phosphatidic acid-preferring phospholipase A1 knock-out mice using targeting vector clone PGS00049_B_D06 and specific siRNA, phenotype, overview
additional information
deletion mutant of H-Rev107 lacking either N-terminal 8 or N-terminal 16 amino acids (DELTAN1, DELTAN2) or C-terminal 25 amino acids (DELTAC1). Expression of the mutants with the expected molecular masses. As for PLA1/2 activity, DELTAN1 shows very low activity (about 3% that of the wild-type), and DELTAN2 and DELTAC1 are entirely inactive
additional information
mutants generated by mutagenic and unidirectional reassembly show lipase activity
additional information
stability and catalytic activity of phospholipase A1 in the presence of an organic solvent is enhanced by error-prone polymerase chanin reaction and DNA shuffling followed by a filter-based visual screening
additional information
thermal stability and catalytic activity of phospholipase A1 are improved by evolutionary molecular engineering
additional information
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enzyme immobilization using Lewatit VP OC 1600 as immobilization carrier, method, n-3 polyunsaturated fatty acids-enriched phosphatidylcholine is successfully produced with fatty acid from fish oil and phosphatidylcholine from soybean by immobilized phospholipase A1-catalyzed acidolysis, overview. The yield of phosphatidylcholine increases significantly by application of vacuum, even though a slight decrease of n-3 polyunsaturated fatty acid incorporation is observed
additional information
construction of the PLA1 deletion mutant cell line BSF lacking enzyme activity, overview
additional information
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construction of the PLA1 deletion mutant cell line BSF lacking enzyme activity, overview
additional information
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construction of the PLA1 deletion mutant cell line BSF lacking enzyme activity, overview
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