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Literature summary for 3.1.1.32 extracted from
- The Rickettsia prowazekii ExoU homologue possesses phospholipase A1 (PLA1), PLA2, and Lyso-PLA2 activities and can function in the absence of any eukaryotic cofactors in vitro (2011), J. Bacteriol., 193, 4634-4642.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | addition of bovine liver superoxide dismutase 1, SOD1, resultes in an increased rate of RP534-catalyzed phospholipid hydrolysis, mechanism, overview | Rickettsia prowazekii |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expresssion of His-tagged wild-type and mutant enzymes in Escherichia coli strain C41[DE3] | Rickettsia prowazekii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D264A | site-directed mutagenesis, substrate specificity compared to the wild-type enzyme, overview | Rickettsia prowazekii |
| S100A | site-directed mutagenesis, substrate specificity compared to the wild-type enzyme, overview | Rickettsia prowazekii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | inhibition of RP534 PLA2 activity by nanomolar amounts of the potent, irreversible inhibitor methyl arachidonoyl fluorophosphonate and by the reversible inhibitor arachidonoyl trifluoromethylketone | Rickettsia prowazekii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Rickettsia prowazekii | RP534 is a bona fide PLA enzyme | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rickettsia prowazekii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain C41[DE3] by nickel affinity chromatography | Rickettsia prowazekii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-acyl-2-oleoyl-sn-glycerophosphocholine + H2O | labeled at the sn-2 position with [14C]oleic acid | Rickettsia prowazekii | acyl-glycerophosphocholine + oleic acid | - |
? | |
| additional information | RP534 is a bona fide PLA enzyme | Rickettsia prowazekii | ? | - |
? | |
| additional information | the Rickettsia prowazekii RP534 protein possesses phospholipase A1, i.e. PLA1, PLA2, and Lyso-PLA2 activities and can function in the absence of any eukaryotic cofactors in vitro, overview. RP534 protein is a homologue of the Pseudomonas aeruginosa ExoU phospholipase A secreted cytotoxin | Rickettsia prowazekii | ? | - |
? | |
| phosphatidylcholine + H2O | labeled at the sn-2 position with [14C]arachadonic acid | Rickettsia prowazekii | 1-acyl-2-oleoyl-sn-glycerophosphocholine + arachidonic acid | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the enzyme belongs to the ExoU family of PLAs | Rickettsia prowazekii |
| RP534 PLA | - |
Rickettsia prowazekii |
| RP534 protein | - |
Rickettsia prowazekii |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | mechanisms of activation of the ExoU family of PLAs may be evolutionarily conserved | Rickettsia prowazekii |
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