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3.1.1.32: phospholipase A1

This is an abbreviated version!
For detailed information about phospholipase A1, go to the full flat file.

Word Map on EC 3.1.1.32

Reaction

phosphatidylcholine
+
H2O
=
2-acylglycerophosphocholine
 +
a carboxylate

Synonyms

Allergen Dol m 1.01, Allergen Dol m 1.02, AtLCAT3, BveA, DAD1, DDHD1, DDL1, endothelial lipase, H-Rev107, hepatic lipase, intracellular phospholipase A1, IPLA-1, lacrimal lipase, lipase H, LipH, LIPI, LPC, membrane-associated phosphatidic acid-selective phospholipase A1, More, mPA-PLA1, mPA-PLA1alpha, mPA-PLA1beta, OM PLA, OMPLA, outer membrane phospholipase A, PA-PLA1, PA-PLA1/DDHD1, pancreatic lipase-associated protein 2, pancreatic lipase-related protein 2, PAPLA1, phosphatidate 1-acylhydorolase, phosphatidic acid phospholipase A1, phosphatidic acid-preferring phospholipase A1, phosphatidic acid-preferring phospholipase A1/DDHD1, phosphatidic acid-selective phospholipase A, phosphatidic acid-specific phospholipase A1, Phosphatidylcholine 1-acylhydrolase, phospholipase A1, phospholipase A1 alpha, phospholipase A1/2, phospholipase A1beta, phospholipase A1gamma, phospholipase beta, Pla, PLA 1, PLA A1, PLA1, PLA1/2, PLA1A, PLA1gamma, PLA1S, Plase A1, PLRP2, PPL, Rab5-activated phospholipase A1, RP534 PLA, RP534 protein, Ves a 1, Ves v 1, vespapase, VesTS1, VipD, Yor022c

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.32 phospholipase A1

Purification

Purification on EC 3.1.1.32 - phospholipase A1

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, DEAE-cellulofine, ether-Toyopearl, hydroxylapatite, Toyopearl HW 50S
-
by nickel-chelating affinity chromatography
-
CM cation-exchange column chromatography and C4 reverse-phase HPLC column chromatography
-
FLAG-tagged H-Rev107 by immunoprecipitation
heparin-Sepharose column chromatography and Superdex 200 gel filtration
-
N156A mutant
-
native enzyme 1500fold from thrombin-activated platelets by anion exchange, hydrophobic interaction, and affinity chromatography
-
native PLA1 21fold from venom by gel filtration and cation exchange chromatography
partial
purified from epimastogote homogenate supernatants, DEAE-Trisacryl, ConA-Sepharose 4B, CM-Sepharose
-
Q sepharose column chromatography
rapid method, suitable for large-scale production
-
recombinant enzyme by ammonium sulfate fractionation, hydrophobic interaction and cation exchange chromatography, followed by anion exchange chromatography
recombinant extracellular enzyme to homogeneity from the culture supernatant by ammonium sulfate precipitation, hydrophobic interaction chromatography, and anion exchange chromatography
recombinant His-tagged phospholipase A1
recombinant His-tagged Vesv5 and Vesv1 wild-type and His-tagged Vesv1 mutant enzymes from Pichia pastoris strains X33 and KM71H from culture supernatant
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain C41[DE3] by nickel affinity chromatography
-
recombinant IPLA-1 from HEK-293 cells
-
recombinant OMPLA
-
recombinant PAL
recombinant phospholipase A
-
recombinant phospholipase A1
recombinant PLA1 expressed in Saccharomyces cerevisiae
-
soluble portion of recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) pLysS by nickel affinity chromatography
Superdex 200, partially purified
-
Triton X-100, Blue-Toyopearl, Red-Toyopearl, DEAE-Toyopearl, Green-agarose, Brown-agarose, Polylysine-agarose, Palmitoyl-CoA-agarose, Blue-5PW
-