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3.1.1.11: pectinesterase

This is an abbreviated version!
For detailed information about pectinesterase, go to the full flat file.

Word Map on EC 3.1.1.11

Reaction

Pectin
+ n H2O = n methanol +
pectate

Synonyms

Ani-PME2, At1g23200, AtPME1, AtPME3, BcPME1, cell wall pectin methylesterase, CsPME, Cthe_2949, CtPME, HMS, hms-1, luPME, MICRO 2676, MICRO 2764, More, Nicotiana tabacum pollen tube PME1, Novoshape, NtPPME1, Ole e 11, Ole e 11.0101, Ole e 11.0102, P65, PE, PE2, pectase, pectin demethoxylase, pectin esterase, pectin methoxylase, pectin methyl esterase, pectin methyl-esterase, pectin methylesterase, pectin methylesterase 3, pectin methylesterase31, pectin methyltesterase, pectine methylesterase, pectinesterase, pectinesterase 31, pectinmethylesterase, pectinoesterase, pectofoetidin, PemB, PEST 1, PEST 2, PME, PME I, PME II, PME III, pme-zj5a, PME1, PME2, PME3, PME31, PME34, PME35, PME4, PME5, PME6, PME7, PME8, PME9, pmeA, pmeB, PMEP, PMEU1, PMT, pollen specific PME, psectin methylesterase, RIP, S6PE, Sal k 1, salt-dependent pectin methylesterase, Sl-PME, thermally tolerant pectin methylesterase, thermolabile pectin methylesterase, TL-PME, TT-PME, type I PME, Xoo, XOO2696

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.11 pectinesterase

Purification

Purification on EC 3.1.1.11 - pectinesterase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
4 enzyme forms: A, B, C and D
-
affinity purification with immobilized pectin methylesterase-inhibitor protein
ammonium sulfate fractionation
-
ammonium sulfate precipitation
-
ammonium sulfate precipitation and DEAE cellulose column chromatography
-
ammonium sulfate precipitation and NH-Sepharose 4B PME-inhibitor column chromatography
-
ammonium sulfate precipitation and Ni-NTA agarose column chromatography
-
ammonium sulfate precipitation, dialysis, and DEAE-Sephadex gel filtration
-
ammonium sulfate precipitation, HisTrap column chromatography, and SOURCE 30Q column chromatography
-
ammonium sulfate precipitation, HiTrap Q column chromatography, and Resource S column chromatography
-
ammonium sulfate precipitation, Q Sepharose column chromatography, and Superdex 200 gel filtration
ammonium sulfate precipitation, Sephadex G-100 gel filtration, and Sephadex C-50 gel filtration
-
DE52 column chromatography, SP-Sepharose column chromatography, Mono-S column chromatography, and Superdex 75 gel filtration
DEAE-Sepharose column chromatography and PME-IP affinity chromatography
-
from fruit rag tissue
-
further purification of a commercial preparation by gel filtration
-
good results of purification is obtained on silanized CPG or keratin coated silica gel supports
-
heparin-Sepharose column chromatography, S-sepharose column chromatography, and Superdex-75 gel filtration
Hi-Trap SP column chromatography and heparin affinity column chromatography
-
homogeneity, one-step purification
-
isoenzyme PE I and isoenzyme PE II
-
isoenzyme PE I and PE II
-
isoenzyme PE II
-
isoenzyme PME I and PME II
-
native enzyme 10.6fold from unripe fruits by anion exchange chromatography and gel filtration
native enzyme from fruits partially by ammonium sufate fractionation and dialysis
native enzyme from pollen by gel filtration, anion exchange chromatography and isoelectric focusing
native enzyme partially from fruits by ammonium sulfate fractionation and dialysis
-
native enzyme partially, 5.8fold by cation exchange chromatography
-
native isozymes about 12fold by ammonium sulfate fractionation, PME inhibitor PMEI affinity chromatography, and cation exchange chromatography
-
native isozymes PME1 389fold and PME2 125fold by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration
-
Ni-NTA column chromatography
Ni-NTA His-bind resin chromatography, DEAE-Sephadex gel filtration, CM-cellulose column chromatography, and Shephacryl SH-100 gel filtration
-
partial
partial purification by ammonium sulfate precipitation and dialysis
-
partial, 3 isoenzymes: I, II and III
-
partial, isoenzyme PE I and PE II
-
partially purified by CS174 EBA resin column chromatography
-
pectinesterase I and II
-
phenyl Sepharose column chromatography and Sephacryl S100 gel filtration
recombinant enzyme from Pichia pastoris cell-free culture medium by anion exchange chromatography and ultrafiltration
recombinant enzyme from Pichia pastoris strain GS115
recombinant enzyme from Pichia pastoris strain GS115 culture medium by dialysis and anion exchange chromatography
recombinant His-tagged enzyme from pastoris strain KM71H by nickel affinity chromatography
recombinant His-tagged isozyme PME31 from Escherichia coli strain JM101 by nickel affinity chromatography
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography and dialysis
recombinant protein and native protein from host
-
resource Q column chromatography
-
Superdex-75 gel filtration
-
two isoforms, homogeneity
-