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Literature summary for 3.1.1.11 extracted from
- Functional expression in pichia pastoris of an acidic pectin methylesterase from jelly fig (Ficus awkeotsang) (2005), J. Agric. Food Chem., 53, 5612-5616.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris as secretory protein | Ficus pumila var. awkeotsang |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
x * 38000, SDS-PAGE, protein from host | Ficus pumila var. awkeotsang |
| 45000 | - |
x * 45000, SDS-PAGE, recombinant protein | Ficus pumila var. awkeotsang |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin + H2O | Ficus pumila var. awkeotsang | involved in the regulation of the cell wall rigidity, key role in process of fruit ripening | methanol + pectate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ficus pumila var. awkeotsang | - |
jelly fig Makino | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | native protein has about 10% carbohydrates, recombinant protein appears to be hyperglycosylated | Ficus pumila var. awkeotsang |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein and native protein from host | Ficus pumila var. awkeotsang |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| achene | - |
Ficus pumila var. awkeotsang | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, fully stable for several months | Ficus pumila var. awkeotsang |
| room temperature, fully stable for several days | Ficus pumila var. awkeotsang |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin + H2O | - |
Ficus pumila var. awkeotsang | methanol + pectate | - |
? | |
| pectin + H2O | involved in the regulation of the cell wall rigidity, key role in process of fruit ripening | Ficus pumila var. awkeotsang | methanol + pectate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 38000, SDS-PAGE, protein from host | Ficus pumila var. awkeotsang |
| ? | x * 45000, SDS-PAGE, recombinant protein | Ficus pumila var. awkeotsang |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pectin methylesterase | - |
Ficus pumila var. awkeotsang |
| PME | - |
Ficus pumila var. awkeotsang |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
no loss of activity within 2 h | Ficus pumila var. awkeotsang |
| 65 | - |
native enzyme stable for 30 min, 60% of activity remaining after 2 h | Ficus pumila var. awkeotsang |
| 70 | - |
20% of activity remaining after 2 h | Ficus pumila var. awkeotsang |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
native and recombinant protein | Ficus pumila var. awkeotsang |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | - |
Ficus pumila var. awkeotsang |
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