Any feedback?
Literature summary for 3.1.1.11 extracted from
- Recombinant expression and inhibition mechanism analysis of pectin methylesterase from Aspergillus flavus (2014), FEMS Microbiol. Lett., 355, 12-19.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris | Aspergillus flavus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (-)-epigallocatechin | 82% residual activity at 20 mg/ml | Aspergillus flavus |  |
| catechinhydrate | 83% residual activity at 20 mg/ml | Aspergillus flavus | |
| epigallocatechin gallate | 47% residual activity at 20 mg/ml | Aspergillus flavus | |
| gallic acid | 80% residual activity at 20 mg/ml | Aspergillus flavus | |
| additional information | 10 mg/ml sucrose and 10 mg/ml coumaric acid have no effect on enzyme activity | Aspergillus flavus | |
| SDS | 78% residual activity at 10 mg/ml SDS | Aspergillus flavus | |
| Tannic acid | 10 mg/ml tannic acid completely inhibits the enzyme | Aspergillus flavus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 40000, SDS-PAGE | Aspergillus flavus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin + H2O | Aspergillus flavus | - |
methanol + pectate | - |
? | |
| pectin + H2O | Aspergillus flavus NRRL3357 | - |
methanol + pectate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus flavus | - |
- |
- |
| Aspergillus flavus NRRL3357 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, HisTrap column chromatography, and SOURCE 30Q column chromatography | Aspergillus flavus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.7 | - |
crude enzyme, at pH 5.0 and 30°C | Aspergillus flavus |
| 194 | - |
after 34fold purification, at pH 5.0 and 30°C | Aspergillus flavus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin + H2O | - |
Aspergillus flavus | methanol + pectate | - |
? | |
| pectin + H2O | - |
Aspergillus flavus NRRL3357 | methanol + pectate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, SDS-PAGE | Aspergillus flavus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pectin methylesterase | - |
Aspergillus flavus |
| PME | - |
Aspergillus flavus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Aspergillus flavus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 65 | the enzyme activity is slightly increased at temperatures above 30°C, but peaks at 55°C. At temperatures above 55°C, the activity drops rapidly, and no activity is detected at 65°C | Aspergillus flavus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | - |
- |
Aspergillus flavus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.2 | 6 | - |
Aspergillus flavus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
