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Literature summary for 3.1.1.11 extracted from
- Pectin methylesterase of Datura species, purification, and characterization from Datura stramonium and its application (2013), Plant Signal. Behav., 8, e25681.
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | due to very high de-esterification activity, easy denaturation and significant efficacy in incrementing clarification of fruit juice makes the enzyme useful for industrial application | Datura inoxia |
| food industry | due to very high de-esterification activity, easy denaturation and significant efficacy in incrementing clarification of fruit juice makes the enzyme useful for industrial application | Datura stramonium |
| food industry | due to very high de-esterification activity, easy denaturation and significant efficacy in incrementing clarification of fruit juice makes the enzyme useful for industrial application | Datura metel |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | optimum activity in the presence of 0.3 M NaCl | Datura inoxia |  |
| NaCl | optimum activity in the presence of 0.3 M NaCl | Datura stramonium | |
| NaCl | optimum activity in the presence of 0.3 M NaCl | Datura metel | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
x * 33000, SDS-PAGE | Datura inoxia |
| 33000 | - |
x * 33000, SDS-PAGE | Datura stramonium |
| 33000 | - |
x * 33000, SDS-PAGE | Datura metel |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin + H2O | Datura inoxia | - |
methanol + pectate | - |
? | |
| pectin + H2O | Datura stramonium | - |
methanol + pectate | - |
? | |
| pectin + H2O | Datura metel | - |
methanol + pectate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Datura inoxia | - |
- |
- |
| Datura metel | - |
- |
- |
| Datura stramonium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, Q Sepharose column chromatography, and Superdex 200 gel filtration | Datura inoxia |
| ammonium sulfate precipitation, Q Sepharose column chromatography, and Superdex 200 gel filtration | Datura stramonium |
| ammonium sulfate precipitation, Q Sepharose column chromatography, and Superdex 200 gel filtration | Datura metel |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| exocarp | highest activity | Datura inoxia | - |
| exocarp | highest activity | Datura stramonium | - |
| exocarp | highest activity | Datura metel | - |
| leaf | - |
Datura inoxia | - |
| leaf | - |
Datura stramonium | - |
| leaf | - |
Datura metel | - |
| seed | - |
Datura inoxia | - |
| seed | - |
Datura stramonium | - |
| seed | - |
Datura metel | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin + H2O | - |
Datura inoxia | methanol + pectate | - |
? | |
| pectin + H2O | - |
Datura stramonium | methanol + pectate | - |
? | |
| pectin + H2O | - |
Datura metel | methanol + pectate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 33000, SDS-PAGE | Datura inoxia |
| ? | x * 33000, SDS-PAGE | Datura stramonium |
| ? | x * 33000, SDS-PAGE | Datura metel |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pectin methylesterase | - |
Datura inoxia |
| pectin methylesterase | - |
Datura stramonium |
| pectin methylesterase | - |
Datura metel |
| PME | - |
Datura inoxia |
| PME | - |
Datura stramonium |
| PME | - |
Datura metel |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Datura inoxia |
| 60 | - |
- |
Datura stramonium |
| 60 | - |
- |
Datura metel |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 90 | maximum activity is observed at 60°C after that activity decreases sharply up to almost zero at 90°C | Datura inoxia |
| 60 | 90 | maximum activity is observed at 60°C. The activity decreases sharply up to almost zero at 90°C | Datura stramonium |
| 60 | 90 | maximum activity is observed at 60°C. The activity decreases sharply up to almost zero at 90°C | Datura metel |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 80 | the enzyme retains more than 90% activity at 60°C for 60 min. At 70°C, the enzyme loses 46 and 61% activity in 30 and 60 min, respectively. Activity is completely abolished at 80°C after 5 min of incubation | Datura inoxia |
| 60 | 80 | the enzyme retains more than 90% activity at 60°C for 60 min. At 70°C, the enzyme loses 46 and 61% activity in 30 and 60 min, respectively. Activity is completely abolished at 80°C after 5 min of incubation | Datura stramonium |
| 60 | 80 | the enzyme retains more than 90% activity at 60°C for 60 min. At 70°C, the enzyme loses 46 and 61% activity in 30 and 60 min, respectively. Activity is completely abolished at 80°C after 5 min of incubation | Datura metel |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Datura inoxia |
| 9 | - |
- |
Datura stramonium |
| 9 | - |
- |
Datura metel |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | high activity is observed between pH 7.0 and 10.0 | Datura inoxia |
| 7 | 10 | high activity is observed between pH 7.0 and 10.0 | Datura stramonium |
| 7 | 10 | high activity is observed between pH 7.010.0 | Datura metel |
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