Literature summary for 3.1.1.11 extracted from

Tu, T.; Bai, Y.; Luo, H.; Ma, R.; Wang, Y.; Shi, P.; Yang, P.; Meng, K.; Yao, B.
A novel bifunctional pectinase from Penicillium oxalicum SX6 with separate pectin methylesterase and polygalacturonase catalytic domains (2014), Appl. Microbiol. Biotechnol., 98, 5019-5028.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Pichia pastoris strain GS115	Penicillium oxalicum

Inhibitors

Inhibitors	Comment	Organism	Structure
beta-mercaptoethanol	96% residual activity at 1 mM	Penicillium oxalicum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	313.2% activity at 1 mM	Penicillium oxalicum
Co2+	351.2% activity at 1 mM	Penicillium oxalicum
Cr3+	216.5% activity at 1 mM	Penicillium oxalicum
Cu2+	104.5% activity at 1 mM	Penicillium oxalicum
EDTA	229% activity at 1 mM	Penicillium oxalicum
Fe3+	107.1% activity at 1 mM	Penicillium oxalicum
K+	126.1% activity at 1 mM	Penicillium oxalicum
Li+	118.2% activity at 1 mM	Penicillium oxalicum
Mg2+	379.5% activity at 1 mM	Penicillium oxalicum
Mn2+	369.9% activity at 1 mM	Penicillium oxalicum
Na+	137.2% activity at 1 mM	Penicillium oxalicum
Ni2+	331.8% activity at 1 mM	Penicillium oxalicum
Pb2+	139.2% activity at 1 mM	Penicillium oxalicum
SDS	105.9% activity at 1 mM	Penicillium oxalicum
Zn2+	324.4% activity at 1 mM	Penicillium oxalicum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38100	-	x * 38100, calculated from amino acid sequence	Penicillium oxalicum
55000	-	x * 55000, SDS-PAGE	Penicillium oxalicum

Organism

Organism	UniProt	Comment	Textmining
Penicillium oxalicum	-	-	-
Penicillium oxalicum SX6	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	-	Penicillium oxalicum

Purification (Commentary)

Purification (Comment)	Organism
resource Q column chromatography	Penicillium oxalicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
68.6	-	at pH 5.0 and 50°C	Penicillium oxalicum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
citrus pectin + H2O	-	Penicillium oxalicum	methanol + pectate	-	?
citrus pectin + H2O	-	Penicillium oxalicum SX6	methanol + pectate	-	?
sisal fibre + H2O	-	Penicillium oxalicum	?	-	?
sisal fibre + H2O	-	Penicillium oxalicum SX6	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 55000, SDS-PAGE	Penicillium oxalicum
?	x * 38100, calculated from amino acid sequence	Penicillium oxalicum

Synonyms

Synonyms	Comment	Organism
pectin methylesterase	-	Penicillium oxalicum
S6PE	catalytic domain of pectin methylesterase	Penicillium oxalicum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Penicillium oxalicum

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
40	55	-	Penicillium oxalicum

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
40	-	the enzyme remains stable for 1 h at 40°C	Penicillium oxalicum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
47.6	-	citrus pectin	at pH 5.0 and 50°C	Penicillium oxalicum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	-	Penicillium oxalicum

pH Range

pH Minimum	pH Maximum	Comment	Organism
4	6	-	Penicillium oxalicum

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Release 2023.1 (January 2023)