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3.1.1.11: pectinesterase

This is an abbreviated version!
For detailed information about pectinesterase, go to the full flat file.

Word Map on EC 3.1.1.11

Reaction

Pectin
+ n H2O = n methanol +
pectate

Synonyms

Ani-PME2, At1g23200, AtPME1, AtPME3, BcPME1, cell wall pectin methylesterase, CsPME, Cthe_2949, CtPME, HMS, hms-1, luPME, MICRO 2676, MICRO 2764, More, Nicotiana tabacum pollen tube PME1, Novoshape, NtPPME1, Ole e 11, Ole e 11.0101, Ole e 11.0102, P65, PE, PE2, pectase, pectin demethoxylase, pectin esterase, pectin methoxylase, pectin methyl esterase, pectin methyl-esterase, pectin methylesterase, pectin methylesterase 3, pectin methylesterase31, pectin methyltesterase, pectine methylesterase, pectinesterase, pectinesterase 31, pectinmethylesterase, pectinoesterase, pectofoetidin, PemB, PEST 1, PEST 2, PME, PME I, PME II, PME III, pme-zj5a, PME1, PME2, PME3, PME31, PME34, PME35, PME4, PME5, PME6, PME7, PME8, PME9, pmeA, pmeB, PMEP, PMEU1, PMT, pollen specific PME, psectin methylesterase, RIP, S6PE, Sal k 1, salt-dependent pectin methylesterase, Sl-PME, thermally tolerant pectin methylesterase, thermolabile pectin methylesterase, TL-PME, TT-PME, type I PME, Xoo, XOO2696

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.11 pectinesterase

Crystallization

Crystallization on EC 3.1.1.11 - pectinesterase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified enzyme in deglycosylated and Asn-linked N-acetylglucosamine-stub forms, hanging drop vapor diffusion method, drops of 0.002 ml from protein and reservoir solutions in the ratios of 1:1 and 2:3, the latter containing 1.9 M (NH4)2SO4, 100 mM sodium acetate, pH 3.6, (EndoHf-deglycosylated Ani-PME2), or 1.8 M (NH4)2SO4, 100 mM sodium acetate, pH 4.1 (PNGaseF-deglycosylated Ani-PME2), 21°C, 1 week, method optimization, X-ray diffraction structure determination and analysis at 1.75-1.80 A resolution, molecular replacement using the structure of PMEs from Solanum lycopersicum (PDB code 1xg2) and Daucus carota (PDB code 1gq8) as search models
hanging drop vapor diffusion method
the inactive D178A PME mutant in complex with specifically methylated hexagalacturonates, 3 mg/ml protein, crystallization solutions contain 0.1 M MES, pH 6.5, 10% dioxane and 1.6 M ammonium sulfate, and dilutions of that crystallisation buffer with H2O, or 0.1 M MES, pH 6.5, and 12% w/v PEG 20000, X-ray diffraction structure determination and anaylsis at 1.7-1.9 A resolution
-
complex between enzyme and PMEI, vapor diffusion technique
sitting drop vapor diffusion method, using 25% (w/v) PEG 3350, 0.1 M citric acid pH 3.5