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Literature summary for 3.1.1.11 extracted from

  • Evangelista, D.E.; Schutzer de Godoy, A.; Fonseca Pereira de Paula, F.; Henrique-Silva, F.; Polikarpov, I.
    Expression, purification, crystallization and preliminary X-ray diffraction analysis of the pectin methylesterase from the sugar cane weevil Sphenophorus levis (2014), Acta Crystallogr. Sect. F, 70, 331-334.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Sphenophorus levis

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 25% (w/v) PEG 3350, 0.1 M citric acid pH 3.5 Sphenophorus levis

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 x * 50000, SDS-PAGE Sphenophorus levis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pectin + H2O Sphenophorus levis
-
 methanol + pectate
-
 ?

Organism

Organism UniProt Comment Textmining
Sphenophorus levis V9Q624 sugar cane weevil
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Sphenophorus levis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pectin + H2O
-
 Sphenophorus levis methanol + pectate
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Sphenophorus levis

Synonyms

Synonyms Comment Organism
pectin methylesterase
-
 Sphenophorus levis
PME
-
 Sphenophorus levis