3.1.1.11: pectinesterase

This is an abbreviated version!
For detailed information about pectinesterase, go to the full flat file.

Reaction

Synonyms

Ani-PME2, At1g23200, AtPME1, AtPME3, BcPME1, cell wall pectin methylesterase, CsPME, Cthe_2949, CtPME, HMS, hms-1, luPME, MICRO 2676, MICRO 2764, More, Nicotiana tabacum pollen tube PME1, Novoshape, NtPPME1, Ole e 11, Ole e 11.0101, Ole e 11.0102, P65, PE, PE2, pectase, pectin demethoxylase, pectin esterase, pectin methoxylase, pectin methyl esterase, pectin methyl-esterase, pectin methylesterase, pectin methylesterase 3, pectin methylesterase31, pectin methyltesterase, pectine methylesterase, pectinesterase, pectinesterase 31, pectinmethylesterase, pectinoesterase, pectofoetidin, PemB, PEST 1, PEST 2, PME, PME I, PME II, PME III, pme-zj5a, PME1, PME2, PME3, PME31, PME34, PME35, PME4, PME5, PME6, PME7, PME8, PME9, pmeA, pmeB, PMEP, PMEU1, PMT, pollen specific PME, psectin methylesterase, RIP, S6PE, Sal k 1, salt-dependent pectin methylesterase, Sl-PME, thermally tolerant pectin methylesterase, thermolabile pectin methylesterase, TL-PME, TT-PME, type I PME, Xoo, XOO2696

ECTree

     3 Hydrolases

         3.1 Acting on ester bonds

             3.1.1 Carboxylic-ester hydrolases

                3.1.1.11 pectinesterase

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Results	in table
24	Activating Compound
20194	AA Sequence
32	Application
1	CAS Registry Number
44	Cloned(Commentary)
6	Crystallization (Commentary)
17	Expression
75	General Information
17	General Stability
224	Inhibitors
5	kcat/KM [mM/s]
3	Ki Value [mM]
28	KM Value [mM]
58	Localization
110	Metals/Ions
109	Molecular Weight [Da]
140	Natural Substrates/ Products (Substrates)
393	Organism
4	Pathway
23	PDB ID
136	pH Optimum
49	pH Range
15	pH Stability
35	pI Value
35	Posttranslational Modification
26	Protein Variants
88	Purification (Commentary)
11	Reaction
3	Reaction Type
354	Reference
202	Source Tissue
76	Specific Activity [micromol/min/mg]
10	Storage Stability
434	Substrates and Products (Substrate)
108	Subunits
481	Synonyms
1	Systematic Name
92	Temperature Optimum [°C]
31	Temperature Range [°C]
143	Temperature Stability [°C]
13	Turnover Number [1/s]

pH Optimum

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pH Optimum on EC 3.1.1.11 - pectinesterase

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pH OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

10

Psidium guajava

-

-

692672

2.5 - 4.5

Athelia rolfsii

-

-

114108, 114109

3.8

Aspergillus niger

A0A345K402

-

751146

4 - 6

Botrytis cinerea

A0A384J5R9

compared to pH 7.5, homogalacturonans as substrates

750823

4.4

Solanum lycopersicum

-

-

679554

4.5

3 entries

4.6

Aspergillus foetidus

-

enzyme immobilized on CNBr-Sepharose 4B or polyethylenen terephthalate

114099

4.7

Aspergillus niger

-

-

730283

4.8

3 entries

5

4 entries

5.3

Citrus sinensis

O04886

assay at

751405

5.5

3 entries

5.5 - 6

2 entries

5.5 - 7

Linum usitatissimum

Q94FS5, Q94FS6, Q9FVF9

neutral isoform

653562

5.5 - 7.5

2 entries

6

7 entries

6 - 7

2 entries

6 - 7.5

3 entries

6.1

Populus tremula x Populus tremuloides

Q9FY03

assay at

682430

6.5

3 entries

6.5 - 7

Diospyros kaki

-

two pH optima

674087

6.5 - 9

Phaseolus vulgaris

-

isoenzyme PE I from pod

114121

6.8

2 entries

6.9 - 7

Fragaria x ananassa

-

-

650676

7

13 entries

7 - 7.5

Acrocylindrium sp.

-

-

114112

7 - 8

Phytophthora infestans

-

isoenzyme II

114102

7 - 9

Phaseolus vulgaris

-

isoenzyme PE II from seed hull

114121

7.2

Solanum lycopersicum

-

at atmospheric pressure and 55°C

651555

7.3

Malus sp.

-

-

114108

7.5

19 entries

7.6

2 entries

7.8

4 entries

7.9

Daucus carota

-

-

114108

8

11 entries

8 - 8.5

3 entries

8 - 9

2 entries

8.3

Malpighia glabra

-

assay at

681639

8.5

4 entries

8.5 - 9

Linum usitatissimum

Q94FS5, Q94FS6, Q9FVF9

very basic isoform, i.e. luPME5

653562

8.5 - 9.5

Citrus sinensis

-

at 50 mM NaCl

651560

9

13 entries

additional information

Aspergillus aculeatus

-

acidic pH optimum

680286

4.5

Aspergillus aculeatus

Q12535

-

714653

4.5

Aspergillus japonicus

-

-

114108, 650276

4.5

Aureobasidium pullulans

-

pectin hydrolase II, at 50°C

114097

4.8

Aspergillus flavus

-

-

729756

4.8

Aspergillus foetidus

-

soluble enzyme

114099

4.8

Coniella diplodiella

-

-

114108

5

Aspergillus oryzae

-

-

114119

5

Dickeya chrysanthemi

-

-

114125

5

Penicillium oxalicum

-

-

729080

5

Sclerotinia libertiana

-

-

114108

5.5

Aspergillus oryzae

-

-

114106

5.5

Aureobasidium pullulans

-

pectin hydrolase I, at 50°C

114097

5.5

Vitis sp.

-

-

114108

5.5 - 6

Citrus sinensis

P83948, Q8GS16

in the presence of 1.2% NaCl

715216

5.5 - 6

Olea europaea

B2VPR8, D8VPP5

-

714956

5.5 - 7.5

Citrus sinensis

-

activity increases with increasing pH up to 45°C

651562

5.5 - 7.5

Solanum lycopersicum

-

activity increases with increasing pH up to 45°C

651562

6

Actinidia chinensis

-

two pH optima

674087

6

Botrytis cinerea

A0A384J5R9

compared to pH 4.0 and pH 7.5, Citrus pectins as substrates

750823

6

Citrus sinensis

-

presence of 1.2% NaCl

651558

6

Ficus pumila var. awkeotsang

-

native and recombinant protein

674101

6

Linum usitatissimum

-

-

114113

6

Musa acuminata

-

-

114108

6

Naganishia albida

-

-

114103

6 - 7

Sitophilus oryzae

-

-

114117

6 - 7

Thanatephorus cucumeris

-

-

114108

6 - 7.5

Arabidopsis thaliana

Q9LVQ0

compared to pH 4.0, homogalacturonans as substrates

750823

6 - 7.5

Citrus sinensis

O04886

compared to pH 4.0, homogalacturonans as substrates

750823

6 - 7.5

Ficus pumila var. awkeotsang

-

-

651557

6.5

Aspergillus pseudoglaucus

-

-

690308

6.5

Lasiodiplodia theobromae

-

-

114108

6.5

Solanum lycopersicum

-

-

730888

6.8

Aspergillus niger

G3YAL0

assay at

751060

6.8

Citrus sinensis

-

assay at

705592

7

Arabidopsis thaliana

-

-

683188

7

Carica papaya

A6YGF1

-

751232

7

Citrus bergamia

Q1PAH6

isozyme PME II, at 0.1 M NaCl isozyme PME III shows two maxima at pH 7.0 and 9.0

692425

7

Citrus sinensis

-

assay at

750603

7

Dickeya chrysanthemi

-

assay at

679502

7

Fusarium oxysporum

-

-

114108

7

Musa acuminata

-

-

674088

7

Phytophthora infestans

-

-

114108

7

Phytophthora infestans

-

isoenzyme PE I or PE II

114095

7

Phytophthora infestans

-

isoenzyme PE I

114102

7

Solanum lycopersicum

-

assay at

680321

7

Sphenophorus levis

V9Q624

recombinant enzyme

750786

7

Theobroma cacao

-

-

114108

7.5

Acrocylindrium sp.

-

-

114108

7.5

Arabidopsis thaliana

O49006

assay at

751100

7.5

Arabidopsis thaliana

Q9LVQ0

compared to pH 4.0 and pH 6.0, very low activity at pH 4.0, Citrus pectins as substrates

750823

7.5

Capsicum annuum

-

at 22°C

674086

7.5

Capsicum frutescens

-

assay at

675652

7.5

Citrus bergamia

Q1PAH6

isozyme PME I

692425

7.5

Citrus sinensis

-

-

114107, 114108

7.5

Citrus sinensis

-

at 0.1-0.2 M NaCl

651560

7.5

Citrus sinensis

O04886

compared to pH 6.0 and pH 4.0, low activity at pH 4.0, Citrus pectins as substrates

750823

7.5

Daucus carota

-

assay at

678803

7.5

Dickeya chrysanthemi

-

-

114129

7.5

Lactuca sativa

-

assay at

678803

7.5

Oculimacula yallundae

-

-

114108

7.5

Phaseolus vulgaris

-

assay at

674118

7.5

Prunus armeniaca

A0A0N9LQL8

-

751233

7.5

Solanum betaceum

-

-

764696

7.5

Solanum lycopersicum

-

-

114108, 729017

7.5

Solanum lycopersicum

-

soluble and immobilized enzyme

114099

7.5

Solanum lycopersicum

-

assay at

674118

7.6

Citrus sinensis

-

isoenzyme I

114108

7.6

Trichoderma reesei

-

-

114101

7.8

Citrus reticulata

-

assay at

679601

7.8

Citrus reticulata � Citrus sinensis

-

assay at

679601

7.8

Citrus sinensis

-

assay at

679601

7.8

Daucus carota

P83218

-

650804

8

Arabidopsis thaliana

-

-

730600

8

Carica papaya

-

-

114104, 114108

8

Carica papaya

-

isoenzyme PME I and PME II

114126

8

Citrus natsudaidai

-

-

114111

8

Citrus sinensis

-

-

675163, 729867

8

Citrus sinensis

-

isoenzyme II

114108

8

Diospyros kaki

-

two pH optima

674087

8

Hippophae rhamnoides subsp. mongolica

-

-

681155

8

Hippophae rhamnoides subsp. rhamnoides

-

-

681155

8

Prunus armeniaca

-

immobilized enzyme

693695

8

Solanum lycopersicum

-

-

650703

8 - 8.5

Actinidia chinensis

-

two pH optima

674087

8 - 8.5

Arabidopsis thaliana

-

in phosphate buffer

694782

8 - 8.5

Solanum lycopersicum

-

-

114108

8 - 9

Dickeya chrysanthemi

-

-

650755

8 - 9

Fragaria x ananassa

-

-

674088

8.5

Acetivibrio thermocellus

A3DJL8

recombinant enzyme

751541

8.5

Aspergillus oryzae

-

-

114105, 114106

8.5

Linum usitatissimum

Q94FS5, Q94FS6, Q9FVF9

basic isoform

653562

8.5

Psidium guajava

-

-

673778

9

Arabidopsis thaliana

-

around pH 9 in glycine buffer

694782

9

Citrus bergamia

Q1PAH6

isozyme PME III, at 0.35 M NaCl only one maximum is observed at pH 9.0

692425

9

Citrus nobilis

-

-

114108

9

Citrus nobilis

-

around

114130

9

Citrus sinensis

-

-

714653

9

Citrus sinensis

-

absence of NaCl

651558

9

Clostridium multifermentans

-

-

114108

9

Datura inoxia

-

-

730650

9

Datura metel

-

-

730650

9

Datura stramonium

-

-

730650

9

Daucus carota

-

-

674088

9

Prunus armeniaca

-

-

694840, 701983

9

Prunus armeniaca

-

free enzyme

693695