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3.1.1.11: pectinesterase

This is an abbreviated version!
For detailed information about pectinesterase, go to the full flat file.

Word Map on EC 3.1.1.11

Reaction

Pectin
+ n H2O = n methanol +
pectate

Synonyms

Ani-PME2, At1g23200, AtPME1, AtPME3, BcPME1, cell wall pectin methylesterase, CsPME, Cthe_2949, CtPME, HMS, hms-1, luPME, MICRO 2676, MICRO 2764, More, Nicotiana tabacum pollen tube PME1, Novoshape, NtPPME1, Ole e 11, Ole e 11.0101, Ole e 11.0102, P65, PE, PE2, pectase, pectin demethoxylase, pectin esterase, pectin methoxylase, pectin methyl esterase, pectin methyl-esterase, pectin methylesterase, pectin methylesterase 3, pectin methylesterase31, pectin methyltesterase, pectine methylesterase, pectinesterase, pectinesterase 31, pectinmethylesterase, pectinoesterase, pectofoetidin, PemB, PEST 1, PEST 2, PME, PME I, PME II, PME III, pme-zj5a, PME1, PME2, PME3, PME31, PME34, PME35, PME4, PME5, PME6, PME7, PME8, PME9, pmeA, pmeB, PMEP, PMEU1, PMT, pollen specific PME, psectin methylesterase, RIP, S6PE, Sal k 1, salt-dependent pectin methylesterase, Sl-PME, thermally tolerant pectin methylesterase, thermolabile pectin methylesterase, TL-PME, TT-PME, type I PME, Xoo, XOO2696

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.11 pectinesterase

Cloned

Cloned on EC 3.1.1.11 - pectinesterase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
diverse isozymes, DNA sequence anaylsis, phylogenetic tree, PME transcriptomes, overview
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DNA and amino acid sequence determination and analysis, expression in Escherichia coli
DNA sequence anaylsis, overview
DNA sequence anaylsis, phylogenetic tree, overview
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DNA sequence anaylsis,phylogenetic tree, PME transcriptomes, overview
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DNA sequence and phylogenetic analysis
expressed as GFP-fusion protein in host strain, overexpression of PME results in reduced pollen tube growth
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expressed in Escherichia coli
expressed in Escherichia coli strain JM101, IPTG induction at 37°C instead of 25-28°C results in approximately 10times less PME activity in the extract
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expressed in Escherichia coli strain M15, the recombinant PME proteins (full-length and mature) do not show either PME or RIP activity
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expressed in Escherichia coli Top10F' cells
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expressed in Nicotiana benthamiana fused with the gene for the green fluorescent protein
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expressed in Nicotiana tabacum fused with the gene for the green fluorescent protein
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expressed in Pichia pastoris
expressed in Pichia pastoris as secretory protein
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expressed in Pichia pastoris strain GS115
expressed in Pichia pastoris strain KM71
expression in Bacillus subtilis
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expression of wild-type and mutant enzymes in Escherichia coli strain NM522
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expression under the control of the CaMV 35S promoter in transgenic Nicotiana tabacum
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gene Cthe_2949, DNA and amino acid sequence determination and analysis and tree, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene Cthe_2949, sequence comparisons and phylogenetic analysis
gene pme-zj5a, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression in Pichia pastoris strain GS115 to 71.11 U/ml after induction with methanol for 20 h at 30°C
gene PME3, quantitative real-time RT-PCR enzyme expression analysis
gene PME6, DNA and amino acid sequence determination and analysis, recombinant expression of YFP-tagged enzyme in the seed coat and cells of the embryos, the HMS-YFP fusions does not have enough HMS activity to compensate for the hms mutation
gene pmeB, DNA and amino acid sequence determination and analysis, recombinant expression in Pichia pastoris, the recombinant enzyme is secreted to the culture medium
gene Pmeu1 encodes a salt-dependent isozyme, expression of PMEU1 and the antisense construct in leaves and fruits of transgenic tomato plants, expression analysis, overview
gene pmt, quantitative realtime PCR expression analysis
in the tomato genome, there exists 79 PMEs with temporally and spatially regulated expression, quantitative realtime PCR expression analysis
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overexpression in Escherichia coli
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overexpression under control of the Aspergilus oryzae TEF1 promoter
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recombinant expression in Pichia pastoris strain GS115, the enzyme is secreted to the culture medium
recombinant expression of wild-type enzyme and mutants in Nicotiana benthamiana leaves via the Agrobacterium tumefaciens strain GV3101 transfection method, quantitative RT-PCR enzyme expression analysis
recombinant overexpression of His-tagged isozyme PME31 in Escherichia coli strain JM101
sequence comparisons of 43 pme genes in Oryza sativa, and phylogenetic analysis
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sequence comparisons, the Sl-pectinase ORF, excluding the coding sequence for signal peptide, is cloned in frame with His-tag into pPICZalphaA and pPICZalphaB expression vectors, recombinant overexpression in Pichia pastoris strain KM71H
transient co-expression of the tobacco enzyme with Tobacco mosaic virus TMV-GFP fusion protein in Nicotiana benthamiana plants via transfection mediated by Agrobacterium tumefaciens strain GV3110, the co-expression results in increased virus-induced RNA silencing with inhibition of GFP production, virus RNA degradation, stimulation of siRNAs production, overview
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YFP-tagged protein expressed in host
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