1.3.1.45: 2'-hydroxyisoflavone reductase

This is an abbreviated version!
For detailed information about 2'-hydroxyisoflavone reductase, go to the full flat file.

Word Map on EC 1.3.1.45

Reaction

vestitone
+
NADP+
=
2'-hydroxyformononetin
+
NADPH
+
H+

Synonyms

CaIRL, GbIRL1, GmIFR, IFR, IRL, isoflavone reductase, isoflavone reductase-like protein, LOC100786578, More, NADPH:2'-hydroxyisoflavone oxidoreductase, Ole e 12, OsIRL, PvIFR1, reductase, 2',7-dihydroxy-4',5'-methylenedioxyisoflavone, reductase, 2'-hydroxyisoflavone

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.45 2'-hydroxyisoflavone reductase

Cloned

Cloned on EC 1.3.1.45 - 2'-hydroxyisoflavone reductase

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CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
cloning of full-length FcIRL, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, overview
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, transient expression of GFP-tagged enzyme in Glycine max protoplasts' cytoplasm, recombinant overexpression of the enzyme under the control of a CaMV35S promoter via Agrobacterium tumefaciens strain LBA4404 and tri-parental mating in transgenic Glycine max plants, quantitative real-time PCR enzyme expression analysis, recombinant expression of Nterminally His6-tagged enzyme in Escherichia coli strain Transetta (DE3)
C6TER4
DNA and amino acid sequence dteremination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) by dialysis, nickel affinity chromatography, and proteolytic digestion of fusion protein with Factor Xa protease
-
expression in Escherichia coli
-
expression in Nicotiana tabacum
-
expression in transgenic Nicotiana tabacum plants under control of the CaMV35S promotor
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gene constructs lacking the promoter of CaIRL are introduced into Agrobacterium tumefaciens strain LBA4404 and used to transform tobacco leaf discs
generating of transgenic rice lines overexpressing the OsIRL gene under an abscisic acid inducible promoter, the OsIRL transgenic rice line activated by abscisic acid treatment is tolerant against methyl viologen and glucose/glucose oxidase-induced stress in rice leaves and suspension-cultured cells, cloning of recombinant enzyme as six-His tagged OsIRL protein for antibody production
into Escherichia coli expression vector pET28a
-
isoflavone reductase-like gene gene by reverse transcription, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, recombinant expression in Escherichia coli strain BL21
recombinantly expressed in Escherichia coli
sequence comparisons and phylogenetic tree, quantitative real-time PCR enzyme expression analysis
-