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Literature summary for 1.3.1.45 extracted from

  • Qian, X.; Xu, S.; Zhao, C.; Meng, H.; Wen, G.
    cDNA cloning and expression analyses of the isoflavone reductase-like gene of Dendrobium officinale (2015), Pak. J. Bot., 47, 1265-1270 .
No PubMed abstract available
Search for more literature for 1.3.1.45

Cloned(Commentary)

Cloned (Comment) Organism
isoflavone reductase-like gene gene by reverse transcription, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, recombinant expression in Escherichia coli strain BL21 Dendrobium catenatum

Organism

Organism UniProt Comment Textmining
Dendrobium catenatum A0A068ERY8 i.e. Dendrobium catenatum, cv. Hongxin 6
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Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme sequence contains two conserved N-glycosylation sites Dendrobium catenatum

Source Tissue

Source Tissue Comment Organism Textmining
bud
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 Dendrobium catenatum
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fruit
-
 Dendrobium catenatum
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meristem
-
 Dendrobium catenatum
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additional information tissue expression levels of the enzyme, overview Dendrobium catenatum
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root
-
 Dendrobium catenatum
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stem leaf
-
 Dendrobium catenatum
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Subunits

Subunits Comment Organism
? x * 34000, about, sequence calculation, x * 33000, recombinant enzyme, SDS-PAGE Dendrobium catenatum
More enzyme domain structure, overview Dendrobium catenatum

Synonyms

Synonyms Comment Organism
isoflavone reductase
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 Dendrobium catenatum
isoflavone reductase-like protein
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 Dendrobium catenatum

Cofactor

Cofactor Comment Organism Structure
NADPH the enzyme contains a NAD(P)H-binding motif (GGTGYIG) in the N-terminal region Dendrobium catenatum

General Information

General Information Comment Organism
additional information the enzyme contains a conserved nitrogen metabolite repression regulator (NmrA) domain and a phenylcoumaran benzylic ether reductase (PCBER) domain Dendrobium catenatum