BRENDA - Enzyme Database show
show all sequences of 1.3.1.45

Overexpression of rice isoflavone reductase-like gene (OsIRL) confers tolerance to reactive oxygen species

Kim, S.G.; Kim, S.T.; Wang, Y.; Kim, S.K.; Lee, C.H.; Kim, K.K.; Kim, J.K.; Lee, S.Y.; Kang, K.Y.; Physiol. Plant. 138, 1-9 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
generating of transgenic rice lines overexpressing the OsIRL gene under an abscisic acid inducible promoter, the OsIRL transgenic rice line activated by abscisic acid treatment is tolerant against methyl viologen and glucose/glucose oxidase-induced stress in rice leaves and suspension-cultured cells, cloning of recombinant enzyme as six-His tagged OsIRL protein for antibody production
Oryza sativa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2'-hydroxyformononetin + NADPH + H+
Oryza sativa
key enzyme in isoflavonoid phytoalexin biosynthesis
vestitone + NADP+
-
-
?
additional information
Oryza sativa
involvement of OsIRL in homeostasis of reactive oxygen species, products of OsIRL are positive regulators to suppress cell death under oxidative stress
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Oryza sativa
-
-
-
Oryza sativa
Q8VYH7
L. cv. Jinheung
-
Purification (Commentary)
Commentary
Organism
as six-His tagged OsIRL protein, the generated recombinant OsIRL protein is used for antibody preparation
Oryza sativa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
callus
-
Oryza sativa
-
cell suspension culture
-
Oryza sativa
-
root
;
Oryza sativa
-
seedling
;
Oryza sativa
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2'-hydroxyformononetin + NADPH + H+
key enzyme in isoflavonoid phytoalexin biosynthesis
700650
Oryza sativa
vestitone + NADP+
-
-
-
?
additional information
involvement of OsIRL in homeostasis of reactive oxygen species, products of OsIRL are positive regulators to suppress cell death under oxidative stress
700650
Oryza sativa
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Oryza sativa
Cloned(Commentary) (protein specific)
Commentary
Organism
generating of transgenic rice lines overexpressing the OsIRL gene under an abscisic acid inducible promoter, the OsIRL transgenic rice line activated by abscisic acid treatment is tolerant against methyl viologen and glucose/glucose oxidase-induced stress in rice leaves and suspension-cultured cells, cloning of recombinant enzyme as six-His tagged OsIRL protein for antibody production
Oryza sativa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Oryza sativa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2'-hydroxyformononetin + NADPH + H+
Oryza sativa
key enzyme in isoflavonoid phytoalexin biosynthesis
vestitone + NADP+
-
-
?
additional information
Oryza sativa
involvement of OsIRL in homeostasis of reactive oxygen species, products of OsIRL are positive regulators to suppress cell death under oxidative stress
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
as six-His tagged OsIRL protein, the generated recombinant OsIRL protein is used for antibody preparation
Oryza sativa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
callus
-
Oryza sativa
-
cell suspension culture
-
Oryza sativa
-
root
-
Oryza sativa
-
seedling
-
Oryza sativa
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2'-hydroxyformononetin + NADPH + H+
key enzyme in isoflavonoid phytoalexin biosynthesis
700650
Oryza sativa
vestitone + NADP+
-
-
-
?
additional information
involvement of OsIRL in homeostasis of reactive oxygen species, products of OsIRL are positive regulators to suppress cell death under oxidative stress
700650
Oryza sativa
?
-
-
-
-
Expression
Organism
Commentary
Expression
Oryza sativa
After embedding of seeds in agar containing 2',7',-dichlorofluorescin, the H2O2 is highly stimulated in the elongation region compared with the root apex. The OsIRL mRNA expression level in 3-day-old roots is weakly expressed in the apex region, it shows an increment accompanying the increase in H2O2 concentration, results indicate that the expression level and localization of OsIRL is associated with H2O2 accumulation.; OsIRL gene and protein are downregulated in young seedling roots treated with diphenyleneiodonium, known quencher of reactive oxygen species, but to a lesser extent when compared with glutathione treatment in roots; OsIRL gene and protein are downregulated in young seedling roots treated with reduced glutathione, a known quencher of reactive oxygen species, in roots of 3 day-old young seedlings grown on glutathione, reactive oxygen species levels are significantly decreased by approximately 50% in the presence of 1 mM glutathione compared with untreated controls; OsIRL gene and protein are shown to be downregulated in young seedling roots treated with reduced glutathione and diphenyleneiodonium
down
Oryza sativa
OsIRL transcript level in rice suspension-cultured cells is found to be by hydrogen peroxide, ferric chloride, methyl viologen and glucose/glucose oxidase; the expression of OsIRL is slightly increased after treatment 0.02 mM abscisic acid in wild-type calli, suggesting a possible reason for glucose/glucose oxidase stress resistance; the OsIRL transcript level in rice suspension-cultured cells is induced by FeCl3 within 4 h after treatment, but down-regulated when co-treated with glutathione; the OsIRL transcript level in rice suspension-cultured cells is induced by glucose/glucose oxidase within 4 h after treatment, but down-regulated when co-treated with glutathione; the OsIRL transcript level in rice suspension-cultured cells is induced by methyl viologen within 4 h after treatment, but down-regulated when co-treated with glutathione; the OsIRL transcript level in rice suspension-cultured cells is induced by the oxidant H2O2, but down-regulated when co-treated with glutathione
up
General Information
General Information
Commentary
Organism
malfunction
transgenic rice lines overexpressing the OsIRL gene under an abscisic acid inducible promoter are tolerant against methyl viologen (MV) and glucose/glucose oxidase-induced stress in rice leave and suspension-cultured cells
Oryza sativa
General Information (protein specific)
General Information
Commentary
Organism
malfunction
transgenic rice lines overexpressing the OsIRL gene under an abscisic acid inducible promoter are tolerant against methyl viologen (MV) and glucose/glucose oxidase-induced stress in rice leave and suspension-cultured cells
Oryza sativa
Expression (protein specific)
Organism
Commentary
Expression
Oryza sativa
OsIRL gene and protein are shown to be downregulated in young seedling roots treated with reduced glutathione and diphenyleneiodonium
down
Oryza sativa
After embedding of seeds in agar containing 2',7',-dichlorofluorescin, the H2O2 is highly stimulated in the elongation region compared with the root apex. The OsIRL mRNA expression level in 3-day-old roots is weakly expressed in the apex region, it shows an increment accompanying the increase in H2O2 concentration, results indicate that the expression level and localization of OsIRL is associated with H2O2 accumulation.; OsIRL gene and protein are downregulated in young seedling roots treated with diphenyleneiodonium, known quencher of reactive oxygen species, but to a lesser extent when compared with glutathione treatment in roots; OsIRL gene and protein are downregulated in young seedling roots treated with reduced glutathione, a known quencher of reactive oxygen species, in roots of 3 day-old young seedlings grown on glutathione, reactive oxygen species levels are significantly decreased by approximately 50% in the presence of 1 mM glutathione compared with untreated controls
down
Oryza sativa
OsIRL transcript level in rice suspension-cultured cells is found to be by hydrogen peroxide, ferric chloride, methyl viologen and glucose/glucose oxidase
up
Oryza sativa
the expression of OsIRL is slightly increased after treatment 0.02 mM abscisic acid in wild-type calli, suggesting a possible reason for glucose/glucose oxidase stress resistance; the OsIRL transcript level in rice suspension-cultured cells is induced by FeCl3 within 4 h after treatment, but down-regulated when co-treated with glutathione; the OsIRL transcript level in rice suspension-cultured cells is induced by glucose/glucose oxidase within 4 h after treatment, but down-regulated when co-treated with glutathione; the OsIRL transcript level in rice suspension-cultured cells is induced by methyl viologen within 4 h after treatment, but down-regulated when co-treated with glutathione; the OsIRL transcript level in rice suspension-cultured cells is induced by the oxidant H2O2, but down-regulated when co-treated with glutathione
up
Other publictions for EC 1.3.1.45
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Overexpression of rice isoflav ...
Oryza sativa
Physiol. Plant.
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2009
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Fagopyrum cymosum
Yao Xue Xue Bao
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809-819
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675379
Wang
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Medicago sativa
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358
1341-1352
2006
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7
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654910
Saunders
-
The characterization of defens ...
Medicago sativa
BioControl
49
715-728
2004
1
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390816
Cooper
-
Biotransformation of an exogen ...
Medicago sativa
Plant Cell Rep.
20
876-884
2002
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656914
Lopez-Meyer
-
Immunolocalization of vestiton ...
Medicago sativa
Physiol. Mol. Plant Pathol.
61
15-30
2002
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390815
Paiva
Molecular cloning of isoflavon ...
Pisum sativum
Arch. Biochem. Biophys.
312
501-510
1994
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390813
Schlieper
-
Stereospecificity of hydrogen ...
Cicer arietinum
Phytochemistry
29
1519-1524
1990
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390814
Preisig
Biosynthesis of the phytoalexi ...
Pisum sativum
Plant Physiol.
94
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1990
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390812
Tiemann
-
Isolation of NADPH:isoflavone ...
Cicer arietinum
FEBS Lett.
213
324-328
1987
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