BRENDA - Enzyme Database show
show all sequences of 1.3.1.45

Expression patterns of an isoflavone reductase-like gene and its possible roles in secondary metabolism in Ginkgo biloba

Hua, C.; Linling, L.; Feng, X.; Yan, W.; Honghui, Y.; Conghua, W.; Shaobing, W.; Zhiqin, L.; Juan, H.; Yuping, W.; Shuiyuan, C.; Fuliang, C.; Plant Cell Rep. 32, 637-650 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
recombinantly expressed in Escherichia coli
Ginkgo biloba
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
predicted from amino acid sequence
Ginkgo biloba
5737
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33280
-
calculated from cDNA
Ginkgo biloba
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ginkgo biloba
M1T9X3
-
-
Purification (Commentary)
Commentary
Organism
using Ni-NTA agarose affinity chromatography
Ginkgo biloba
Source Tissue
Source Tissue
Commentary
Organism
Textmining
root
-
Ginkgo biloba
-
seedling
-
Ginkgo biloba
-
stem
-
Ginkgo biloba
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dehydrodiconiferyl alcohol + NADPH
-
726152
Ginkgo biloba
isodihydrodehydrodiconiferyl alcohol + NADP+
-
-
-
?
isodihydrodehydrodiconiferyl alcohol + NADPH
-
726152
Ginkgo biloba
tetrahydrodehydrodiconiferyl alcohol + NADP+
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Ginkgo biloba
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
assay at
Ginkgo biloba
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Ginkgo biloba
NADPH
-
Ginkgo biloba
Cloned(Commentary) (protein specific)
Commentary
Organism
recombinantly expressed in Escherichia coli
Ginkgo biloba
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Ginkgo biloba
NADPH
-
Ginkgo biloba
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
predicted from amino acid sequence
Ginkgo biloba
5737
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33280
-
calculated from cDNA
Ginkgo biloba
Purification (Commentary) (protein specific)
Commentary
Organism
using Ni-NTA agarose affinity chromatography
Ginkgo biloba
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
root
-
Ginkgo biloba
-
seedling
-
Ginkgo biloba
-
stem
-
Ginkgo biloba
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dehydrodiconiferyl alcohol + NADPH
-
726152
Ginkgo biloba
isodihydrodehydrodiconiferyl alcohol + NADP+
-
-
-
?
isodihydrodehydrodiconiferyl alcohol + NADPH
-
726152
Ginkgo biloba
tetrahydrodehydrodiconiferyl alcohol + NADP+
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Ginkgo biloba
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
assay at
Ginkgo biloba
Expression
Organism
Commentary
Expression
Ginkgo biloba
increased expression of GbIRL1 is detected when the seedlings are treated with ultraviolet-B, 5-aminolevulinic acid, wounding and ethephon, abscisic acid, salicylic acid
up
Expression (protein specific)
Organism
Commentary
Expression
Ginkgo biloba
increased expression of GbIRL1 is detected when the seedlings are treated with ultraviolet-B, 5-aminolevulinic acid, wounding and ethephon, abscisic acid, salicylic acid
up
Other publictions for EC 1.3.1.45
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744406
Castro
Immunoproteomic tools are use ...
Olea europaea
Biochim. Biophys. Acta
1854
1871-1880
2015
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1
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1
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1
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1
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1
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1
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1
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2
2
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-
744991
Cheng
Overexpression of soybean iso ...
Glycine max
Front. Plant Sci.
6
1024
2015
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1
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1
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1
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1
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4
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2
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2
2
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1
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1
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2
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2
2
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1
1
2
2
1
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745962
Qian
-
cDNA cloning and expression a ...
Dendrobium catenatum
Pak. J. Bot.
47
1265-1270
2015
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1
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1
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6
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1
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6
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2
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-
-
-
-
-
-
1
1
-
-
-
726152
Hua
Expression patterns of an isof ...
Ginkgo biloba
Plant Cell Rep.
32
637-650
2013
-
-
1
-
-
-
-
-
1
-
1
-
-
4
-
-
1
-
-
3
-
-
2
-
1
-
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1
-
-
2
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-
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1
2
-
-
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-
-
-
-
1
-
1
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-
-
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1
-
3
-
-
2
-
1
-
-
-
1
-
-
-
1
-
-
1
-
-
746131
Ripodas
Knock-down of a member of the ...
Phaseolus vulgaris
Plant Physiol. Biochem.
68
81-89
2013
-
-
1
-
1
-
-
-
-
-
-
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4
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2
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2
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-
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-
-
-
-
-
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-
1
3
3
1
-
-
700650
Kim
Overexpression of rice isoflav ...
Oryza sativa
Physiol. Plant.
138
1-9
2009
-
-
1
-
-
-
-
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2
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3
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1
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4
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2
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1
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1
1
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2
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1
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6
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2
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2
1
1
4
-
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700726
Brandalise
The promoter of a gene encodin ...
Coffea arabica
Plant Cell Rep.
28
1699-1708
2009
-
-
1
-
-
-
-
-
-
-
1
1
-
3
-
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-
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2
1
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1
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1
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1
1
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1
1
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2
1
-
1
-
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-
-
-
-
-
-
-
1
-
-
1
-
-
713596
Zhu
Molecular cloning and characte ...
Fagopyrum cymosum
Yao Xue Xue Bao
44
809-819
2009
-
-
1
-
-
-
1
-
-
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-
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1
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3
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1
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1
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1
1
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1
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3
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1
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1
1
-
-
-
675379
Wang
Crystal structure of isoflavon ...
Medicago sativa
J. Mol. Biol.
358
1341-1352
2006
-
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1
1
8
-
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7
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3
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1
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1
1
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7
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1
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1
1
1
8
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7
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1
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1
1
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7
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-
-
-
-
654910
Saunders
-
The characterization of defens ...
Medicago sativa
BioControl
49
715-728
2004
1
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1
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1
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1
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2
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1
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1
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1
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1
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2
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-
390816
Cooper
-
Biotransformation of an exogen ...
Medicago sativa
Plant Cell Rep.
20
876-884
2002
-
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1
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-
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-
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1
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1
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1
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1
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-
-
-
656914
Lopez-Meyer
-
Immunolocalization of vestiton ...
Medicago sativa
Physiol. Mol. Plant Pathol.
61
15-30
2002
1
-
1
-
-
-
-
-
1
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1
-
1
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1
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6
-
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2
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1
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1
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1
1
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1
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1
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1
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6
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2
-
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-
390815
Paiva
Molecular cloning of isoflavon ...
Pisum sativum
Arch. Biochem. Biophys.
312
501-510
1994
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1
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-
-
-
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5
-
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1
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1
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1
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-
-
390813
Schlieper
-
Stereospecificity of hydrogen ...
Cicer arietinum
Phytochemistry
29
1519-1524
1990
-
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-
-
-
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1
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2
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390814
Preisig
Biosynthesis of the phytoalexi ...
Pisum sativum
Plant Physiol.
94
1444-1448
1990
-
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-
-
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-
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-
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2
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1
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1
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1
1
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1
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1
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-
390812
Tiemann
-
Isolation of NADPH:isoflavone ...
Cicer arietinum
FEBS Lett.
213
324-328
1987
-
-
-
-
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2
1
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1
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1
1
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1
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1
1
1
3
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1
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1
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2
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2
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2
1
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1
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1
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1
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1
1
1
3
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1
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1
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