BRENDA - Enzyme Database show
show all sequences of 1.3.1.45

Molecular cloning and characterization of a novel isoflavone reductase-like gene (FcIRL) from high flavonoids-producing callus of Fagopyrum cymosum

Zhu, Q.L.; Guo, T.Y.; Sui, S.Z.; Liu, G.D.; Lei, X.H.; Luo, L.L.; Li, M.Y.; Yao Xue Xue Bao 44, 809-819 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
cloning of full-length FcIRL, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, overview
Fagopyrum cymosum
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
the enzyme sequence contains a conserved NmrA, nitrogen metabolite repression regulator, domain, V6-N244
Fagopyrum cymosum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Fagopyrum cymosum
A7Y232
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
glycoprotein
the enzyme sequence contains one conserved glycosylation site at N214
Fagopyrum cymosum
additional information
the enzyme sequence contains an N-terminal acetylation site M1-K5
Fagopyrum cymosum
phosphoprotein
the enzyme sequence contains multi-phosphorylation sites
Fagopyrum cymosum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
callus
-
Fagopyrum cymosum
-
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
the enzyme sequence contains a NADPH-binding motif, G10GTG13YIG16, in the N-terminal region
Fagopyrum cymosum
Cloned(Commentary) (protein specific)
Commentary
Organism
cloning of full-length FcIRL, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, overview
Fagopyrum cymosum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
the enzyme sequence contains a NADPH-binding motif, G10GTG13YIG16, in the N-terminal region
Fagopyrum cymosum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
the enzyme sequence contains a conserved NmrA, nitrogen metabolite repression regulator, domain, V6-N244
Fagopyrum cymosum
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
glycoprotein
the enzyme sequence contains one conserved glycosylation site at N214
Fagopyrum cymosum
additional information
the enzyme sequence contains an N-terminal acetylation site M1-K5
Fagopyrum cymosum
phosphoprotein
the enzyme sequence contains multi-phosphorylation sites
Fagopyrum cymosum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
callus
-
Fagopyrum cymosum
-
General Information
General Information
Commentary
Organism
metabolism
FcIRL belongs to the class of pinoresinol-lariciresinol reductase, PRL, functioning in the biosynthesis of 8,8'-linked lignans, with function in catalyzing reduction of pinoresinol and lariciresinol into secoisolariciresinol, and medicinal secondary metabolism and resistance in Fagopyrum cymosum
Fagopyrum cymosum
General Information (protein specific)
General Information
Commentary
Organism
metabolism
FcIRL belongs to the class of pinoresinol-lariciresinol reductase, PRL, functioning in the biosynthesis of 8,8'-linked lignans, with function in catalyzing reduction of pinoresinol and lariciresinol into secoisolariciresinol, and medicinal secondary metabolism and resistance in Fagopyrum cymosum
Fagopyrum cymosum
Other publictions for EC 1.3.1.45
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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726152
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746131
Ripodas
Knock-down of a member of the ...
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Plant Physiol. Biochem.
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2013
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Kim
Overexpression of rice isoflav ...
Oryza sativa
Physiol. Plant.
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2009
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1
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2009
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713596
Zhu
Molecular cloning and characte ...
Fagopyrum cymosum
Yao Xue Xue Bao
44
809-819
2009
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675379
Wang
Crystal structure of isoflavon ...
Medicago sativa
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2006
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654910
Saunders
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The characterization of defens ...
Medicago sativa
BioControl
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2004
1
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390816
Cooper
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Medicago sativa
Plant Cell Rep.
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2002
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656914
Lopez-Meyer
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Immunolocalization of vestiton ...
Medicago sativa
Physiol. Mol. Plant Pathol.
61
15-30
2002
1
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390815
Paiva
Molecular cloning of isoflavon ...
Pisum sativum
Arch. Biochem. Biophys.
312
501-510
1994
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390813
Schlieper
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Stereospecificity of hydrogen ...
Cicer arietinum
Phytochemistry
29
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1990
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390814
Preisig
Biosynthesis of the phytoalexi ...
Pisum sativum
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94
1444-1448
1990
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390812
Tiemann
-
Isolation of NADPH:isoflavone ...
Cicer arietinum
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213
324-328
1987
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