BRENDA - Enzyme Database show
show all sequences of 1.3.1.45

Immunoproteomic tools are used to identify masked allergens Ole e 12, an allergenic isoflavone reductase from olive (Olea europaea) pollen

Castro, L.; Crespo, J.F.; Rodriguez, J.; Rodriguez, R.; Villalba, M.; Biochim. Biophys. Acta 1854, 1871-1880 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
DNA and amino acid sequence dteremination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) by dialysis, nickel affinity chromatography, and proteolytic digestion of fusion protein with Factor Xa protease
Olea europaea
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Olea europaea
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged enzyme from cell free extract of Escherichia coli strain BL21(DE3)
Olea europaea
Subunits
Subunits
Commentary
Organism
?
x * 37000, SDS-PAGE, x * 34285, recombinant His-tagged enzyme, mass spectrometry
Olea europaea
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Olea europaea
sequence calculation
-
5.7
Cloned(Commentary) (protein specific)
Commentary
Organism
DNA and amino acid sequence dteremination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) by dialysis, nickel affinity chromatography, and proteolytic digestion of fusion protein with Factor Xa protease
Olea europaea
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme from cell free extract of Escherichia coli strain BL21(DE3)
Olea europaea
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 37000, SDS-PAGE, x * 34285, recombinant His-tagged enzyme, mass spectrometry
Olea europaea
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Olea europaea
sequence calculation
-
5.7
General Information
General Information
Commentary
Organism
evolution
the allergen, named Ole e 12, is a polymorphic isoflavone reductase-like protein of 308 amino acids showing 80% and 74% identity with birch and pear allergens, Bet v 6 and Pyr c 5, respectively. Analysis of the presence of Ole e 12 homologues in different pollens from other species, overview
Olea europaea
physiological function
the 37-kDa enzyme protein seems to be associated to the IgE-binding profile of a group of patients suffering allergy to peach and olive pollen. The allergen, named Ole e 12, is a polymorphic isoflavone reductase-like protein of 308 amino acids showing 80% and 74% identity with birch and pear allergens, Bet v 6 and Pyr c 5, respectively. Ole e 12 is a minor olive pollen allergen, which gains relevance in patients allergic to peach with olive pollinosis
Olea europaea
General Information (protein specific)
General Information
Commentary
Organism
evolution
the allergen, named Ole e 12, is a polymorphic isoflavone reductase-like protein of 308 amino acids showing 80% and 74% identity with birch and pear allergens, Bet v 6 and Pyr c 5, respectively. Analysis of the presence of Ole e 12 homologues in different pollens from other species, overview
Olea europaea
physiological function
the 37-kDa enzyme protein seems to be associated to the IgE-binding profile of a group of patients suffering allergy to peach and olive pollen. The allergen, named Ole e 12, is a polymorphic isoflavone reductase-like protein of 308 amino acids showing 80% and 74% identity with birch and pear allergens, Bet v 6 and Pyr c 5, respectively. Ole e 12 is a minor olive pollen allergen, which gains relevance in patients allergic to peach with olive pollinosis
Olea europaea
Other publictions for EC 1.3.1.45
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744406
Castro
Immunoproteomic tools are use ...
Olea europaea
Biochim. Biophys. Acta
1854
1871-1880
2015
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744991
Cheng
Overexpression of soybean iso ...
Glycine max
Front. Plant Sci.
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1024
2015
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745962
Qian
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cDNA cloning and expression a ...
Dendrobium catenatum
Pak. J. Bot.
47
1265-1270
2015
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Hua
Expression patterns of an isof ...
Ginkgo biloba
Plant Cell Rep.
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637-650
2013
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746131
Ripodas
Knock-down of a member of the ...
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Plant Physiol. Biochem.
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Kim
Overexpression of rice isoflav ...
Oryza sativa
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Molecular cloning and characte ...
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Yao Xue Xue Bao
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Wang
Crystal structure of isoflavon ...
Medicago sativa
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2006
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654910
Saunders
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The characterization of defens ...
Medicago sativa
BioControl
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2004
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390816
Cooper
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Biotransformation of an exogen ...
Medicago sativa
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876-884
2002
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656914
Lopez-Meyer
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Immunolocalization of vestiton ...
Medicago sativa
Physiol. Mol. Plant Pathol.
61
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2002
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390815
Paiva
Molecular cloning of isoflavon ...
Pisum sativum
Arch. Biochem. Biophys.
312
501-510
1994
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5
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390813
Schlieper
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Stereospecificity of hydrogen ...
Cicer arietinum
Phytochemistry
29
1519-1524
1990
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390814
Preisig
Biosynthesis of the phytoalexi ...
Pisum sativum
Plant Physiol.
94
1444-1448
1990
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390812
Tiemann
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Isolation of NADPH:isoflavone ...
Cicer arietinum
FEBS Lett.
213
324-328
1987
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