BRENDA - Enzyme Database show
show all sequences of 1.3.1.45

Crystal structure of isoflavone reductase from alfalfa (Medicago sativa L.)

Wang, X.; He, X.; Lin, J.; Shao, H.; Chang, Z.; Dixon, R.A.; J. Mol. Biol. 358, 1341-1352 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
into Escherichia coli expression vector pET28a
Medicago sativa
Crystallization (Commentary)
Crystallization
Organism
at 1.6 A resolution by hanging-drop vapor-diffusion method
Medicago sativa
Engineering
Amino acid exchange
Commentary
Organism
D285G
decreased turnover rate
Medicago sativa
DELTA39-47
nine of the amino acid residues around position 40 deleted in the current construct D39-47 IFR to facilitate crystallization, affects enzyme activity only slightly
Medicago sativa
G14F
no detectable activity
Medicago sativa
H164A
decreased turnover rate
Medicago sativa
K144A
complete loss of enzyme activity
Medicago sativa
R97G
decreased turnover rate
Medicago sativa
Y169A
decreased turnover rate
Medicago sativa
Y272A
decreased turnover rate
Medicago sativa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0142
-
2'-hydroxyformononetin
wild-type
Medicago sativa
0.0157
-
2'-hydroxyformononetin
mutant Y272A
Medicago sativa
0.0158
-
2'-hydroxyformononetin
mutant R97G
Medicago sativa
0.0162
-
2'-hydroxyformononetin
mutant DELTA39-47 IFR
Medicago sativa
0.0166
-
2'-hydroxyformononetin
mutant Y169A
Medicago sativa
0.018
-
2'-hydroxyformononetin
mutant D285G
Medicago sativa
0.0182
-
2'-hydroxyformononetin
mutant H164A
Medicago sativa
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Medicago sativa
-
-
-
Purification (Commentary)
Commentary
Organism
Ni2+-NTA affinity chromatography and gel filtration
Medicago sativa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2'-hydroxyformononetin + NADPH
-
675379
Medicago sativa
vestitone + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
gel filtration
Medicago sativa
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.00383
-
2'-hydroxyformononetin
mutant Y272A
Medicago sativa
0.004167
-
2'-hydroxyformononetin
mutant D285G
Medicago sativa
0.0067
-
2'-hydroxyformononetin
mutant Y169A
Medicago sativa
0.0097
-
2'-hydroxyformononetin
mutant R97G
Medicago sativa
0.00983
-
2'-hydroxyformononetin
mutant H164A
Medicago sativa
0.063
-
2'-hydroxyformononetin
mutant DELTA39-47 IFR
Medicago sativa
0.215
-
2'-hydroxyformononetin
wild-type
Medicago sativa
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Medicago sativa
Cloned(Commentary) (protein specific)
Commentary
Organism
into Escherichia coli expression vector pET28a
Medicago sativa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Medicago sativa
Crystallization (Commentary) (protein specific)
Crystallization
Organism
at 1.6 A resolution by hanging-drop vapor-diffusion method
Medicago sativa
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D285G
decreased turnover rate
Medicago sativa
DELTA39-47
nine of the amino acid residues around position 40 deleted in the current construct D39-47 IFR to facilitate crystallization, affects enzyme activity only slightly
Medicago sativa
G14F
no detectable activity
Medicago sativa
H164A
decreased turnover rate
Medicago sativa
K144A
complete loss of enzyme activity
Medicago sativa
R97G
decreased turnover rate
Medicago sativa
Y169A
decreased turnover rate
Medicago sativa
Y272A
decreased turnover rate
Medicago sativa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0142
-
2'-hydroxyformononetin
wild-type
Medicago sativa
0.0157
-
2'-hydroxyformononetin
mutant Y272A
Medicago sativa
0.0158
-
2'-hydroxyformononetin
mutant R97G
Medicago sativa
0.0162
-
2'-hydroxyformononetin
mutant DELTA39-47 IFR
Medicago sativa
0.0166
-
2'-hydroxyformononetin
mutant Y169A
Medicago sativa
0.018
-
2'-hydroxyformononetin
mutant D285G
Medicago sativa
0.0182
-
2'-hydroxyformononetin
mutant H164A
Medicago sativa
Purification (Commentary) (protein specific)
Commentary
Organism
Ni2+-NTA affinity chromatography and gel filtration
Medicago sativa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2'-hydroxyformononetin + NADPH
-
675379
Medicago sativa
vestitone + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
gel filtration
Medicago sativa
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.00383
-
2'-hydroxyformononetin
mutant Y272A
Medicago sativa
0.004167
-
2'-hydroxyformononetin
mutant D285G
Medicago sativa
0.0067
-
2'-hydroxyformononetin
mutant Y169A
Medicago sativa
0.0097
-
2'-hydroxyformononetin
mutant R97G
Medicago sativa
0.00983
-
2'-hydroxyformononetin
mutant H164A
Medicago sativa
0.063
-
2'-hydroxyformononetin
mutant DELTA39-47 IFR
Medicago sativa
0.215
-
2'-hydroxyformononetin
wild-type
Medicago sativa
Other publictions for EC 1.3.1.45
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744406
Castro
Immunoproteomic tools are use ...
Olea europaea
Biochim. Biophys. Acta
1854
1871-1880
2015
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6
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1
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1
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1
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1
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1
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1
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2
2
-
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-
744991
Cheng
Overexpression of soybean iso ...
Glycine max
Front. Plant Sci.
6
1024
2015
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-
1
-
1
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1
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1
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4
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2
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2
2
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2
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1
1
2
2
1
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745962
Qian
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cDNA cloning and expression a ...
Dendrobium catenatum
Pak. J. Bot.
47
1265-1270
2015
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1
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6
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2
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1
1
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-
726152
Hua
Expression patterns of an isof ...
Ginkgo biloba
Plant Cell Rep.
32
637-650
2013
-
-
1
-
-
-
-
-
1
-
1
-
-
4
-
-
1
-
-
3
-
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2
-
1
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1
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2
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1
2
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1
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1
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1
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3
-
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2
-
1
-
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-
1
-
-
-
1
-
-
1
-
-
746131
Ripodas
Knock-down of a member of the ...
Phaseolus vulgaris
Plant Physiol. Biochem.
68
81-89
2013
-
-
1
-
1
-
-
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-
-
-
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4
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2
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1
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2
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-
-
-
-
-
-
1
3
3
1
-
-
700650
Kim
Overexpression of rice isoflav ...
Oryza sativa
Physiol. Plant.
138
1-9
2009
-
-
1
-
-
-
-
-
-
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2
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3
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1
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4
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2
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1
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1
1
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2
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1
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6
-
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2
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-
-
-
-
-
2
1
1
4
-
-
700726
Brandalise
The promoter of a gene encodin ...
Coffea arabica
Plant Cell Rep.
28
1699-1708
2009
-
-
1
-
-
-
-
-
-
-
1
1
-
3
-
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2
1
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1
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1
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1
1
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1
1
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2
1
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
713596
Zhu
Molecular cloning and characte ...
Fagopyrum cymosum
Yao Xue Xue Bao
44
809-819
2009
-
-
1
-
-
-
1
-
-
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1
-
3
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1
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1
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1
1
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1
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3
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1
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-
-
-
-
-
-
-
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-
-
1
1
-
-
-
675379
Wang
Crystal structure of isoflavon ...
Medicago sativa
J. Mol. Biol.
358
1341-1352
2006
-
-
1
1
8
-
-
7
-
-
-
-
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3
-
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1
-
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-
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1
1
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-
7
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1
-
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1
1
1
8
-
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7
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1
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1
1
-
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7
-
-
-
-
-
-
-
-
-
-
654910
Saunders
-
The characterization of defens ...
Medicago sativa
BioControl
49
715-728
2004
1
-
-
-
-
-
-
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1
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1
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1
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2
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1
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1
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1
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1
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1
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2
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-
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-
390816
Cooper
-
Biotransformation of an exogen ...
Medicago sativa
Plant Cell Rep.
20
876-884
2002
-
-
1
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-
-
-
-
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-
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1
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1
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1
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1
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-
656914
Lopez-Meyer
-
Immunolocalization of vestiton ...
Medicago sativa
Physiol. Mol. Plant Pathol.
61
15-30
2002
1
-
1
-
-
-
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-
1
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1
-
1
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1
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6
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2
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1
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1
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1
1
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1
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1
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1
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6
-
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2
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-
-
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-
390815
Paiva
Molecular cloning of isoflavon ...
Pisum sativum
Arch. Biochem. Biophys.
312
501-510
1994
-
-
1
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-
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-
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5
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1
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1
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1
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-
-
-
390813
Schlieper
-
Stereospecificity of hydrogen ...
Cicer arietinum
Phytochemistry
29
1519-1524
1990
-
-
-
-
-
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1
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2
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-
390814
Preisig
Biosynthesis of the phytoalexi ...
Pisum sativum
Plant Physiol.
94
1444-1448
1990
-
-
-
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2
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1
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1
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1
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-
390812
Tiemann
-
Isolation of NADPH:isoflavone ...
Cicer arietinum
FEBS Lett.
213
324-328
1987
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2
1
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1
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3
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1
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1
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2
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2
1
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1
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1
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3
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