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Literature summary for 1.3.1.45 extracted from

  • Wang, X.; He, X.; Lin, J.; Shao, H.; Chang, Z.; Dixon, R.A.
    Crystal structure of isoflavone reductase from alfalfa (Medicago sativa L.) (2006), J. Mol. Biol., 358, 1341-1352.
    View publication on PubMed
Search for more literature for 1.3.1.45

Cloned(Commentary)

Cloned (Comment) Organism
into Escherichia coli expression vector pET28a Medicago sativa

Crystallization (Commentary)

Crystallization (Comment) Organism
at 1.6 A resolution by hanging-drop vapor-diffusion method Medicago sativa

Protein Variants

Protein Variants Comment Organism
D285G decreased turnover rate Medicago sativa
DELTA39-47 nine of the amino acid residues around position 40 deleted in the current construct D39-47 IFR to facilitate crystallization, affects enzyme activity only slightly Medicago sativa
G14F no detectable activity Medicago sativa
H164A decreased turnover rate Medicago sativa
K144A complete loss of enzyme activity Medicago sativa
R97G decreased turnover rate Medicago sativa
Y169A decreased turnover rate Medicago sativa
Y272A decreased turnover rate Medicago sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0142
-
 2'-hydroxyformononetin wild-type Medicago sativa
0.0157
-
 2'-hydroxyformononetin mutant Y272A Medicago sativa
0.0158
-
 2'-hydroxyformononetin mutant R97G Medicago sativa
0.0162
-
 2'-hydroxyformononetin mutant DELTA39-47 IFR Medicago sativa
0.0166
-
 2'-hydroxyformononetin mutant Y169A Medicago sativa
0.018
-
 2'-hydroxyformononetin mutant D285G Medicago sativa
0.0182
-
 2'-hydroxyformononetin mutant H164A Medicago sativa

Organism

Organism UniProt Comment Textmining
Medicago sativa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA affinity chromatography and gel filtration Medicago sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-hydroxyformononetin + NADPH
-
 Medicago sativa vestitone + NADP+
-
 ?

Subunits

Subunits Comment Organism
monomer gel filtration Medicago sativa

Synonyms

Synonyms Comment Organism
IFR
-
 Medicago sativa
isoflavone reductase
-
 Medicago sativa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00383
-
 2'-hydroxyformononetin mutant Y272A Medicago sativa
0.004167
-
 2'-hydroxyformononetin mutant D285G Medicago sativa
0.0067
-
 2'-hydroxyformononetin mutant Y169A Medicago sativa
0.0097
-
 2'-hydroxyformononetin mutant R97G Medicago sativa
0.00983
-
 2'-hydroxyformononetin mutant H164A Medicago sativa
0.063
-
 2'-hydroxyformononetin mutant DELTA39-47 IFR Medicago sativa
0.215
-
 2'-hydroxyformononetin wild-type Medicago sativa

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Medicago sativa