BRENDA - Enzyme Database show
show all sequences of 1.3.1.45

Overexpression of soybean isoflavone reductase (GmIFR) enhances resistance to phytophthora sojae in soybean

Cheng, Q.; Li, N.; Dong, L.; Zhang, D.; Fan, S.; Jiang, L.; Wang, X.; Xu, P.; Zhang, S.; Front. Plant Sci. 6, 1024 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, transient expression of GFP-tagged enzyme in Glycine max protoplasts' cytoplasm, recombinant overexpression of the enzyme under the control of a CaMV35S promoter via Agrobacterium tumefaciens strain LBA4404 and tri-parental mating in transgenic Glycine max plants, quantitative real-time PCR enzyme expression analysis, recombinant expression of Nterminally His6-tagged enzyme in Escherichia coli strain Transetta (DE3)
Glycine max
Engineering
Amino acid exchange
Commentary
Organism
additional information
enzyme overexpression in Glycine max transgenic plants results in highly reduced daidzein levels, while the relative content of glyceollins in transgenic plants is significantly higher than that of non-transgenic plants. The relative expression levels of reactive oxygen species (ROS) of transgenic soybean plants are significantly lower than those of non-transgenic plants after incubation with Phytophthora sojae. Overexpression of GmIFR in soybean seed affects isoflavone and glyceollins expression levels, as well as the transcriptional level of multiple genes involved in the phenylpropanal pathway
Glycine max
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
subcellular localization analysis in transgenic protoplasts
Glycine max
5737
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2'-hydroxyformononetin + NADPH + H+
Glycine max
GmIFR is an isoflavone reductase
vestitone + NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Glycine max
C6TER4
cv. Suinong10
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
hypocotyl
-
Glycine max
-
seedling
-
Glycine max
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2'-hydroxyformononetin + NADPH + H+
-
744991
Glycine max
vestitone + NADP+
-
-
-
?
2'-hydroxyformononetin + NADPH + H+
GmIFR is an isoflavone reductase
744991
Glycine max
vestitone + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 34920, sequence calculation, x * 34000, recombinant enzyme, SDS-PAGE
Glycine max
More
The predicted three-dimensional model prediction
Glycine max
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Glycine max
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Glycine max
sequence calculation
-
6.33
Cloned(Commentary) (protein specific)
Commentary
Organism
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, transient expression of GFP-tagged enzyme in Glycine max protoplasts' cytoplasm, recombinant overexpression of the enzyme under the control of a CaMV35S promoter via Agrobacterium tumefaciens strain LBA4404 and tri-parental mating in transgenic Glycine max plants, quantitative real-time PCR enzyme expression analysis, recombinant expression of Nterminally His6-tagged enzyme in Escherichia coli strain Transetta (DE3)
Glycine max
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Glycine max
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
enzyme overexpression in Glycine max transgenic plants results in highly reduced daidzein levels, while the relative content of glyceollins in transgenic plants is significantly higher than that of non-transgenic plants. The relative expression levels of reactive oxygen species (ROS) of transgenic soybean plants are significantly lower than those of non-transgenic plants after incubation with Phytophthora sojae. Overexpression of GmIFR in soybean seed affects isoflavone and glyceollins expression levels, as well as the transcriptional level of multiple genes involved in the phenylpropanal pathway
Glycine max
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
subcellular localization analysis in transgenic protoplasts
Glycine max
5737
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2'-hydroxyformononetin + NADPH + H+
Glycine max
GmIFR is an isoflavone reductase
vestitone + NADP+
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
hypocotyl
-
Glycine max
-
seedling
-
Glycine max
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2'-hydroxyformononetin + NADPH + H+
-
744991
Glycine max
vestitone + NADP+
-
-
-
?
2'-hydroxyformononetin + NADPH + H+
GmIFR is an isoflavone reductase
744991
Glycine max
vestitone + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 34920, sequence calculation, x * 34000, recombinant enzyme, SDS-PAGE
Glycine max
More
The predicted three-dimensional model prediction
Glycine max
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Glycine max
sequence calculation
-
6.33
Expression
Organism
Commentary
Expression
Glycine max
the enzyme expression is significantly induced by infection with Phytophthora sojae, as well as by treatment with ethephon, abscisic acid, and salicylic acid
up
General Information
General Information
Commentary
Organism
evolution
isoflavone reductases (IFRs) are unique to the plant kingdom. The enzyme is a member of the soybean isoflavone reductase gene family GmIF
Glycine max
physiological function
isoflavonereductase (IFR)is an enzyme involved in the biosynthetic pathway of isoflavonoid phytoalexin in plants. The enzyme is considered to have a crucial role in plant response to various biotic and abiotic environmental stresses. Overexpression of soybean isoflavone reductase (GmIFR) enhances resistance to Phytophthora sojae in soybean. Enzyme GmIFR might play an important role as an antioxidant to reduce ROS in soybean
Glycine max
General Information (protein specific)
General Information
Commentary
Organism
evolution
isoflavone reductases (IFRs) are unique to the plant kingdom. The enzyme is a member of the soybean isoflavone reductase gene family GmIF
Glycine max
physiological function
isoflavonereductase (IFR)is an enzyme involved in the biosynthetic pathway of isoflavonoid phytoalexin in plants. The enzyme is considered to have a crucial role in plant response to various biotic and abiotic environmental stresses. Overexpression of soybean isoflavone reductase (GmIFR) enhances resistance to Phytophthora sojae in soybean. Enzyme GmIFR might play an important role as an antioxidant to reduce ROS in soybean
Glycine max
Expression (protein specific)
Organism
Commentary
Expression
Glycine max
the enzyme expression is significantly induced by infection with Phytophthora sojae, as well as by treatment with ethephon, abscisic acid, and salicylic acid
up
Other publictions for EC 1.3.1.45
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744406
Castro
Immunoproteomic tools are use ...
Olea europaea
Biochim. Biophys. Acta
1854
1871-1880
2015
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2
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744991
Cheng
Overexpression of soybean iso ...
Glycine max
Front. Plant Sci.
6
1024
2015
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745962
Qian
-
cDNA cloning and expression a ...
Dendrobium catenatum
Pak. J. Bot.
47
1265-1270
2015
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726152
Hua
Expression patterns of an isof ...
Ginkgo biloba
Plant Cell Rep.
32
637-650
2013
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746131
Ripodas
Knock-down of a member of the ...
Phaseolus vulgaris
Plant Physiol. Biochem.
68
81-89
2013
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1
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1
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4
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1
3
3
1
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700650
Kim
Overexpression of rice isoflav ...
Oryza sativa
Physiol. Plant.
138
1-9
2009
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2
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2
1
1
4
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700726
Brandalise
The promoter of a gene encodin ...
Coffea arabica
Plant Cell Rep.
28
1699-1708
2009
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1
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1
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3
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1
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713596
Zhu
Molecular cloning and characte ...
Fagopyrum cymosum
Yao Xue Xue Bao
44
809-819
2009
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1
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675379
Wang
Crystal structure of isoflavon ...
Medicago sativa
J. Mol. Biol.
358
1341-1352
2006
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1
1
8
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7
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8
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7
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1
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7
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654910
Saunders
-
The characterization of defens ...
Medicago sativa
BioControl
49
715-728
2004
1
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390816
Cooper
-
Biotransformation of an exogen ...
Medicago sativa
Plant Cell Rep.
20
876-884
2002
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1
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656914
Lopez-Meyer
-
Immunolocalization of vestiton ...
Medicago sativa
Physiol. Mol. Plant Pathol.
61
15-30
2002
1
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390815
Paiva
Molecular cloning of isoflavon ...
Pisum sativum
Arch. Biochem. Biophys.
312
501-510
1994
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390813
Schlieper
-
Stereospecificity of hydrogen ...
Cicer arietinum
Phytochemistry
29
1519-1524
1990
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390814
Preisig
Biosynthesis of the phytoalexi ...
Pisum sativum
Plant Physiol.
94
1444-1448
1990
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390812
Tiemann
-
Isolation of NADPH:isoflavone ...
Cicer arietinum
FEBS Lett.
213
324-328
1987
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