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(polyphosphate)n + H2O
(polyphosphate)(n-1) + phosphate
-
-
-
?
(polyphosphate)n + H2O
(polyphosphate)n-1 + phosphate
-
-
-
?
1,N6-ethenoadenosine 5'-phosphate + H2O
1,N6-ethenoadenosine ribonucleoside + phosphate
-
45% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
?
1-glycerophosphate + H2O
glycerol + phosphate
-
1% of the activity with 5-IMP
-
-
?
2'-chloro-2'-deoxyAMP + H2O
2'-chloro-2'-deoxyadenosine + phosphate
-
cNII
-
-
?
2-(cyclohexylethylthio)adenosine 5'-monophosphate + H2O
2-(cyclohexylethylthio)adenosine + phosphate
-
-
-
-
?
2-(cyclohexylmethylthio)adenosine 5'-monophosphate + H2O
2-(cyclohexylmethylthio)adenosine + phosphate
-
-
-
-
?
2-(cyclohexylthio)adenosine 5'-monophosphate + H2O
2-(cyclohexylthio)adenosine + phosphate
-
-
-
-
?
2-(cyclopentylthio)adenosine 5'-monophosphate + H2O
2-(cyclopentylthio)adenosine + phosphate
-
-
-
-
?
2-(dioxanylethylthio)adenosine 5'-monophosphate + H2O
2-(dioxanylethylthio)adenosine + phosphate
-
-
-
-
?
2-(dioxolanylethylthio)adenosine 5'-monophosphate + H2O
2-(dioxolanylethylthio)adenosine + phosphate
-
-
-
-
?
2-(hexylthio)adenosine 5'-monophosphate + H2O
2-(hexylthio)adenosine + phosphate
-
-
-
-
?
2-(propylthio)adenosine 5'-monophosphate + H2O
2-(propylthio)adenosine + phosphate
-
-
-
-
?
2-chloro-2'-deoxyadenosine monophosphate + H2O
2-chloro-2'-deoxyadenosine + phosphate
39% of the activity with 5'-AMP
-
?
3'-AMP + H2O
adenosine + phosphate
-
-
-
?
3'-azido-3'deoxy-dTMP + H2O
3'-azido-3'-deoxythymidine + phosphate
-
cNII
-
-
?
3'-CMP + H2O
cytosine + phosphate
-
-
-
?
3'-IMP + H2O
inosine + phosphate
-
1% of the activity with 5'-IMP
-
?
3-beta-ribofuranosyladenine 5'-monophosphate + H2O
3-beta-ribofuranosyladenine + phosphate
-
24% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
?
4-methylumbelliferyl phosphate + H2O
4-methylumbelliferol + phosphate
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
5'-ADP + H2O
AMP + phosphate
very low activity
-
-
?
5'-AMP + H2O
adenosine + phosphate
5'-ATP + H2O
ADP + phosphate
very low activity
-
-
?
5'-CMP + H2O
cytidine + phosphate
5'-CMP + xanthosine
XMP + cytidine
-
-
-
-
?
5'-CTP + H2O
CDP + phosphate
low activity
-
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
5'-dCMP + H2O
2'-deoxycytidine + phosphate
5'-dCMP + H2O
deoxycytidine + phosphate
5'-dGMP + H2O
deoxyguanosine + phosphate
5'-dIMP + H2O
deoxyinosine + phosphate
5'-dTMP + H2O
deoxythymidine + phosphate
5'-dUMP + H2O
deoxyuridine + phosphate
5'-GDP + H2O
GMP + phosphate
very low activity
-
-
?
5'-GMP + H2O
guanosine + phosphate
5'-GMP + xanthosine
XMP + guanosine
-
-
-
-
?
5'-GTP + H2O
GDP + phosphate
very low activity
-
-
?
5'-IDP + H2O
IMP + phosphate
very low activity
-
-
?
5'-IMP + H2O
inosine + phosphate
5'-IMP + xanthosine
XMP + inosine
-
-
-
-
?
5'-inosinic acid + H2O
?
-
-
-
-
?
5'-TMP + H2O
thymidine + phosphate
5'-UMP + H2O
uridine + phosphate
5'-UMP + xanthosine
XMP + uridine
-
-
-
-
?
5'-XMP + H2O
xanthosine + phosphate
5-fluoro-dUMP + H2O
5-fluoro-uridine + phosphate
-
cNII
-
-
?
5-phosphoribosyl-1-diphosphate + H2O
?
-
-
-
?
6-chloropurine ribonucleoside 5'-monophosphate + H2O
6-chloropurine ribonucleoside + phosphate
-
50% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
?
7-methylguanosine diphosphate + H2O
7-methylguanosine monophosphate + phosphate
-
preferred substrate
-
-
?
7-methylguanosine monophosphate + H2O
7-methylguanosine + phosphate
-
preferred substrate
-
-
?
9-beta-D-arabinosyl-2-fluoroadenine monophosphate + H2O
9-beta-D-arabinosyl-2-fluoroadenine + phosphate
-
-
-
-
?
a 5'-ribonucleotide + H2O
a ribonucleoside + phosphate
adenosine 5'-O-thiophosphate + H2O
?
-
6% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
-
?
ADP + 2 H2O
adenosine + 2 phosphate
ADP + H2O
5'-AMP + phosphate
ADP + H2O
adenosine + diphosphate
ADP + H2O
AMP + phosphate
-
-
-
-
?
AMP + H2O
adenosine + phosphate
ATP + 3 H2O
adenosine + 3 phosphate
ATP + H2O
adenosine + triphosphate
-
-
-
?
ATP + H2O
ADP + phosphate
bis-p-nitrophenylphosphate + H2O
?
-
-
-
-
?
CMP + H2O
cytidine + phosphate
-
-
-
?
CTP + H2O
?
-
30% of the activity with ATP
-
-
?
cyclic 3',5'-AMP + H2O
?
-
8.8% of the activity with 5'-AMP
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
very low activity
-
-
?
D-ribose 5-phosphate + H2O
D-ribose + phosphate
dAMP + H2O
deoxyadenosine + phosphate
91% of the activity with 5'-AMP
-
?
dATP + H2O
?
-
83% of the activity with ATP
-
-
?
dCMP + H2O
deoxycytidine + phosphate
dGMP + H2O
deoxyguanosine + phosphate
75% of the activity with 5'-AMP
-
?
dIMP + H2O
deoxyinosine + phosphate
diphosphate + H2O
2 phosphate
dTMP + H2O
thymidine + phosphate
-
7.5% of the activity with 5'-IMP
-
?
dUMP + H2O
deoxyuridine + phosphate
7% of the activity with 5'-AMP
-
?
FAD + H2O
?
-
microsomal enzyme
-
-
?
GDP + H2O
?
-
135% of the activity with ATP
-
-
?
GMP + H2O
guanosine + phosphate
-
-
-
?
GTP + H2O
?
-
100% of the activity with ATP
-
-
?
IMP + H2O
inosine + phosphate
-
-
-
-
?
inosine + 5'-IMP
5'-IMP + inosine
-
-
-
?
inosine + phosphate
5'-IMP + H2O
ITP + H2O
?
-
108% of the activity with ATP
-
-
?
ITP + H2O
IDP + phosphate
very low activity
-
-
?
methylthio-IMP + H2O
? + phosphate
-
-
-
-
?
p-nitrophenyl phenylphosphonate + H2O
?
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
p-nitrophenylphosphate + H2O
p-nitrophenol + phosphate
-
plasma membrane enzyme: 1% of the activity with 5'-AMP, Golgi apparatus enzyme: 39% of the activity with 5'-AMP
-
?
ribavirin + 5'-IMP
?
better substrate than viramidine
-
-
?
thio-GMP + H2o
6-thioguanosine + phosphate
-
-
-
-
?
thio-IMP + H2O
6-thioinosine + phosphate
-
-
-
-
?
TTP + H2O
?
-
133% of the activity with ATP
-
-
?
UDP + H2O
?
-
150% of the activity with ATP
-
-
?
UMP + H2O
uridine + phosphate
-
-
-
?
UTP + H2O
?
-
53% of the activity with ATP
-
-
?
UTP + H2O
UDP + phosphate
very low activity
-
-
?
viramidine + 5'-IMP
?
worse substrate than ribavirin
-
-
?
XMP + H2O
xanthosine + phosphate
-
-
-
?
additional information
?
-
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
best substrate, very high activity
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
about 55% of the activity with 4-methylumbelliferyl phosphate
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
5'-AMP + H2O
?
-
enzyme in the system for the hydrolysis of extracellular nucleotides so that product nucleosides are readily transported into the cell
-
-
?
5'-AMP + H2O
?
-
the enzyme is responsible for the production of adenosine in the cardiac tissue
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
best substrate for enzyme from plasma membrane and Golgi apparatus
-
?
5'-AMP + H2O
adenosine + phosphate
-
highly specific, no hydrolysis of other nucleotides
-
?
5'-AMP + H2O
adenosine + phosphate
-
highly specific, no hydrolysis of other nucleotides
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
Bacillus sp. (in: Bacteria) No. A-59
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
low activity
-
-
?
5'-AMP + H2O
adenosine + phosphate
low activity
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
Bothrops pradoi
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
absence of ADP, 60% loss of activity
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
17% of the activity with 5-IMP
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
101% of the activity of 5'-IMP
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
best substrate
-
-
?
5'-AMP + H2O
adenosine + phosphate
about 35% of the activity with 4-methylumbelliferyl phosphate
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
664632, 677295, 677542, 678418, 679669, 681753, 682200, 682217, 690362, 691078, 691082, 692002, 692592, 693385, 694138, 716355, 752167 -
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
best substrate
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
preferred substrate
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
preferred substrate for cNI and eNs
-
?
5'-AMP + H2O
adenosine + phosphate
-
enzyme is responsible for generation of adenosine from AMP. Adenosine is involved in a number of cardioprotective mechanisms. TNF-alpha may reduce the capacity of human endothelial cels to produce adenosine by a decrease in activity of ecto-5'-nucleotidase. The loss of ecto-5'-nucleotidase may lead to decreased production of anti-inflammatory adenosine and retention of proinflammatory nucleotides
-
?
5'-AMP + H2O
adenosine + phosphate
-
CD73
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
best substrate
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
36.3% of the activity with 5'-IMP
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
664550, 677490, 677509, 678104, 679085, 679669, 681724, 681894, 681897, 682113, 682115, 691398, 692192, 693953, 694138, 715146, 715987, 751791, 752167 -
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
100% activity
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
most efficiently hydrolyzed substrate
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
64% of the activity of ATP
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
30% of the activity with 5-IMP
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
best substrate
-
-
?
5'-AMP + H2O
adenosine + phosphate
best substrate
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
21% of the activity with 5'-IMP
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
enzyme is monospecific for AMP among nucleoside 5-monophosphates
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
100% activity
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
100% activity
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
32% of the activity with 5-IMP
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
23.1% of the activity with 5'-AMP
-
?
5'-CMP + H2O
cytidine + phosphate
-
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
125% of the activity with 5'-AMP
-
?
5'-CMP + H2O
cytidine + phosphate
-
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
61.7% of the activity with 5'-AMP
-
?
5'-CMP + H2O
cytidine + phosphate
-
21% of the activity with 5-IMP
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
49% of activity with Mg2+-AMP
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
85% of the activity of 5'-IMP
-
?
5'-CMP + H2O
cytidine + phosphate
-
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
-
-
-
?
5'-CMP + H2O
cytidine + phosphate
about 30% of the activity with 4-methylumbelliferyl phosphate
-
?
5'-CMP + H2O
cytidine + phosphate
-
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
-
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
-
-
?
5'-CMP + H2O
cytidine + phosphate
85% of the activity with 5'-AMP
-
?
5'-CMP + H2O
cytidine + phosphate
-
CD73
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
56.2% of the activity with 5'-AMP
-
?
5'-CMP + H2O
cytidine + phosphate
-
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
93% of the activity with 5'-AMP
-
?
5'-CMP + H2O
cytidine + phosphate
-
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
18.4% of the activity with 5'-IMP
-
?
5'-CMP + H2O
cytidine + phosphate
-
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
87% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
?
5'-CMP + H2O
cytidine + phosphate
-
65% of the activity with 5'-AMP, brain cytosolic enzyme
-
?
5'-CMP + H2O
cytidine + phosphate
-
36% dephosphorylation in relation to AMP
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
Sdt1
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
17% of the activity with 5-IMP
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
48% of the activity with 5'-IMP
-
?
5'-CMP + H2O
cytidine + phosphate
-
93% of the activity with 5'-AMP
-
?
5'-CMP + H2O
cytidine + phosphate
-
87% activity compared to 5'-AMP
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
87% activity compared to 5'-AMP
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
11% of the activity with 5-IMP
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
116% of the activity with 5'-AMP
-
?
5'-CMP + H2O
cytidine + phosphate
-
46% of the activity with 5'-AMP
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
19.7% of the activity with 5'-AMP
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
Bacillus sp. (in: Bacteria) No. A-59
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
Bacillus sp. (in: Bacteria) No. A-59
-
19.7% of the activity with 5'-AMP
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
low activity
-
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
low activity
-
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
22% of the activity with 5'-AMP
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
66% of the activity with 5'-AMP
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
presence of ADP, 66% of the activity with 5-AMP
-
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
-
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
53% of the activity of 5'-IMP
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
-
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
54% of the activity with 5'-AMP
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
20% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
7% of the activity with 5'-AMP, brain cytosolic enzyme
-
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
-
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
-
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
i.e. 2'-deoxyadenosine 5'-phosphate
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
-
14% of the activity with 5'-AMP
-
?
5'-dCMP + H2O
2'-deoxycytidine + phosphate
-
-
-
?
5'-dCMP + H2O
2'-deoxycytidine + phosphate
-
-
-
-
?
5'-dCMP + H2O
2'-deoxycytidine + phosphate
-
CD73
-
-
?
5'-dCMP + H2O
deoxycytidine + phosphate
-
presence of ADP, 16% of the activity with 5-AMP
-
-
?
5'-dCMP + H2O
deoxycytidine + phosphate
-
-
-
?
5'-dCMP + H2O
deoxycytidine + phosphate
-
-
-
-
?
5'-dCMP + H2O
deoxycytidine + phosphate
-
i.e. 2'-deoxycytosine 5'-phosphate
-
?
5'-dCMP + H2O
deoxycytidine + phosphate
-
i.e. 2'-deoxycytosine 5'-phosphate
-
?
5'-dCMP + H2O
deoxycytidine + phosphate
-
i.e. 2'-deoxycytosine 5'-phosphate
-
?
5'-dCMP + H2O
deoxycytidine + phosphate
-
i.e. 2'-deoxycytosine 5'-phosphate
-
?
5'-dCMP + H2O
deoxycytidine + phosphate
-
28% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
?
5'-dCMP + H2O
deoxycytidine + phosphate
-
i.e. 2'-deoxycytosine 5'-phosphate
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
low activity
-
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
i.e. 2'-deoxyguanosine 5'-phosphate
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
49.9% of the activity with 5'-AMP
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
-
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
i.e. 2'-deoxyguanosine 5'-phosphate
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
-
-
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
-
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
i.e. 2'-deoxyguanosine 5'-phosphate
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
cNII
-
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
-
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
-
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
i.e. 2'-deoxyguanosine 5'-phosphate
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
35% of the activity with 5'-AMP
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
i.e. 2'-deoxyguanosine 5'-phosphate
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
i.e. 2'-deoxyguanosine 5'-phosphate
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
-
i.e. 2'-deoxyguanosine 5'-phosphate
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
low activity
-
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
i.e. 2'-deoxyinosine 5'-phosphate
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
60.9% of the activity with 5'-AMP
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
presence of ADP, 38% of the activity with 5-AMP
-
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
-
-
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
102% of the activity with 5-IMP
-
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
-
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
-
-
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
i.e. 2'-deoxyinosine 5'-phosphate
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
i.e. 2'-deoxyinosine 5'-phosphate
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
23% of the activity with 5'-IMP, cytoplasmic enzyme
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
61% of the activity with 5'-AMP
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
cNII
-
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
-
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
-
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
i.e. 2'-deoxyinosine 5'-phosphate
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
95% of the activity with 5-IMP
-
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
99% of the activity with 5-IMP
-
-
?
5'-dIMP + H2O
deoxyinosine + phosphate
-
-
-
-
?
5'-dTMP + H2O
deoxythymidine + phosphate
-
7.1% of the activity with 5-IMP
-
-
?
5'-dTMP + H2O
deoxythymidine + phosphate
-
-
-
?
5'-dTMP + H2O
deoxythymidine + phosphate
-
-
-
-
?
5'-dTMP + H2O
deoxythymidine + phosphate
-
CD73
-
-
?
5'-dTMP + H2O
deoxythymidine + phosphate
-
i.e. 2'-deoxythymidine 5'-phosphate
-
?
5'-dTMP + H2O
deoxythymidine + phosphate
-
-
-
-
?
5'-dTMP + H2O
deoxythymidine + phosphate
-
i.e. 2'-deoxythymidine 5'-phosphate
-
?
5'-dTMP + H2O
deoxythymidine + phosphate
-
i.e. 2'-deoxythymidine 5'-phosphate
-
?
5'-dTMP + H2O
deoxythymidine + phosphate
-
55% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
?
5'-dTMP + H2O
deoxythymidine + phosphate
-
17.5% of the activity with 5-IMP
-
-
?
5'-dTMP + H2O
deoxythymidine + phosphate
-
10% of the activity with 5-IMP
-
-
?
5'-dUMP + H2O
deoxyuridine + phosphate
-
-
-
?
5'-dUMP + H2O
deoxyuridine + phosphate
-
-
-
-
?
5'-dUMP + H2O
deoxyuridine + phosphate
-
i.e. 2'-deoxyuridine 5'-phosphate
-
?
5'-dUMP + H2O
deoxyuridine + phosphate
-
CD73
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
plasma membrane enzyme: 2.85% of the activity with 5'-AMP, Golgi apparatus enzyme: 46% of the activity with 5'-AMP
-
?
5'-GMP + H2O
guanosine + phosphate
-
16.7% of the activity with 5'-AMP
-
?
5'-GMP + H2O
guanosine + phosphate
Bacillus sp. (in: Bacteria) No. A-59
-
16.7% of the activity with 5'-AMP
-
?
5'-GMP + H2O
guanosine + phosphate
low activity
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
117% of the activity with 5'-AMP
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
33.5% of the activity with 5'-AMP
-
?
5'-GMP + H2O
guanosine + phosphate
-
89% of the activity with 5-IMP
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
107% of activity with Mg2+-AMP
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
52% of the activity of 5'-IMP
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
about 30% of the activity with 4-methylumbelliferyl phosphate
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
r
5'-GMP + H2O
guanosine + phosphate
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
preferred substrate
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
85% of the activity with 5'-IMP, cytoplasmic enzyme
-
?
5'-GMP + H2O
guanosine + phosphate
57% of the activity with 5'-AMP
-
?
5'-GMP + H2O
guanosine + phosphate
-
cNII, CD73
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
53.6% of the activity with 5'-AMP
-
?
5'-GMP + H2O
guanosine + phosphate
preferred substrate. Substrate GMP acting as an activator, partitioning between the allosteric and active site, is the basis for the sigmoidicity of the initial velocity versus GMP concentration plot
-
-
?
5'-GMP + H2O
guanosine + phosphate
preferred substrate. Substrate GMP acting as an activator, partitioning between the allosteric and active site, is the basis for the sigmoidicity of the initial velocity versus GMP concentration plot
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
120% of the activity with 5'-AMP
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
97.6% of the activity with 5'-IMP
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
50% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
?
5'-GMP + H2O
guanosine + phosphate
-
19% of the activity with 5'-AMP
-
?
5'-GMP + H2O
guanosine + phosphate
-
57% dephosphorylation in relation to AMP
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
67% of the activity of ATP
-
?
5'-GMP + H2O
guanosine + phosphate
-
99% of the activity with 5-IMP
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
94% of the activity with 5'-IMP
-
?
5'-GMP + H2O
guanosine + phosphate
-
52% of the activity with 5'-AMP
-
?
5'-GMP + H2O
guanosine + phosphate
-
68% activity compared to 5'-AMP
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
68% activity compared to 5'-AMP
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
104% of the activity with 5-IMP
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
116% of the activity with 5'-AMP
-
?
5'-IMP + H2O
?
-
-
-
-
?
5'-IMP + H2O
?
-
the enzyme is principally responsible for dephosphorylation of 5'-IMP but may have some role in adenosine formation
-
-
?
5'-IMP + H2O
inosine + phosphate
low activity
-
-
?
5'-IMP + H2O
inosine + phosphate
-
-
-
-
?
5'-IMP + H2O
inosine + phosphate
-
-
-
?
5'-IMP + H2O
inosine + phosphate
-
-
-
-
r
5'-IMP + H2O
inosine + phosphate
-
-
-
?
5'-IMP + H2O
inosine + phosphate
-
preferred substrate of cytosolic thymus enzyme
-
-
?
5'-IMP + H2O
inosine + phosphate
-
-
-
?
5'-IMP + H2O
inosine + phosphate
-
92% of the activity with 5'-AMP
-
?
5'-IMP + H2O
inosine + phosphate
-
-
-
-
?
5'-IMP + H2O
inosine + phosphate
-
33.1% of the activity with 5'-AMP
-
-
?
5'-IMP + H2O
inosine + phosphate
-
-
-
-
?
5'-IMP + H2O
inosine + phosphate
-
-
-
-
?
5'-IMP + H2O
inosine + phosphate
-
equally reactive as 5'-AMP
-
?
5'-IMP + H2O
inosine + phosphate
-
-
-
-
?
5'-IMP + H2O
inosine + phosphate
-
-
-
-
?
5'-IMP + H2O
inosine + phosphate
-
-
-
-
?
5'-IMP + H2O
inosine + phosphate
-
-
-
?
5'-IMP + H2O
inosine + phosphate
-
-
-
?
5'-IMP + H2O
inosine + phosphate
-
-
-
r
5'-IMP + H2O
inosine + phosphate
-
-
-
?
5'-IMP + H2O
inosine + phosphate
best substrate
-
-
r
5'-IMP + H2O
inosine + phosphate
-
preferred substrate
-
-
?
5'-IMP + H2O
inosine + phosphate
preferred substrate
-
-
?
5'-IMP + H2O
inosine + phosphate
-
preferred substrate for cNII
-
?
5'-IMP + H2O
inosine + phosphate
-
cNII, CD73
-
-
?
5'-IMP + H2O
inosine + phosphate
-
-
-
?
5'-IMP + H2O
inosine + phosphate
-
-
-
?
5'-IMP + H2O
inosine + phosphate
-
148% of the activity with 5'-AMP
-
?
5'-IMP + H2O
inosine + phosphate
-
-
-
?
5'-IMP + H2O
inosine + phosphate
-
-
-
?
5'-IMP + H2O
inosine + phosphate
-
-
-
?
5'-IMP + H2O
inosine + phosphate
-
-
-
?
5'-IMP + H2O
inosine + phosphate
-
-
-
-
?
5'-IMP + H2O
inosine + phosphate
-
36% of the activity with 5'-AMP, brain cytosolic enzyme
-
-
?
5'-IMP + H2O
inosine + phosphate
-
55% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
-
?
5'-IMP + H2O
inosine + phosphate
-
-
-
?
5'-IMP + H2O
inosine + phosphate
-
Isn1
-
-
?
5'-IMP + H2O
inosine + phosphate
-
-
-
-
?
5'-IMP + H2O
inosine + phosphate
-
-
-
-
?
5'-IMP + H2O
inosine + phosphate
-
-
-
?
5'-IMP + H2O
inosine + phosphate
-
-
-
-
?
5'-IMP + H2O
inosine + phosphate
-
63% of the activity with 5'-AMP
-
?
5'-IMP + H2O
inosine + phosphate
-
-
-
?
5'-IMP + H2O
inosine + phosphate
-
-
-
?
5'-IMP + H2O
inosine + phosphate
-
-
-
-
?
5'-IMP + H2O
inosine + phosphate
-
8% of the activity with 5-IMP
-
-
?
5'-TMP + H2O
thymidine + phosphate
-
7.4% of the activity with 5'-AMP
-
?
5'-TMP + H2O
thymidine + phosphate
-
15.2% of the activity with 5'-AMP
-
-
?
5'-TMP + H2O
thymidine + phosphate
about 15% of the activity with 4-methylumbelliferyl phosphate
-
?
5'-TMP + H2O
thymidine + phosphate
-
-
-
?
5'-TMP + H2O
thymidine + phosphate
13% of the activity with 5'-AMP
-
?
5'-TMP + H2O
thymidine + phosphate
-
-
-
-
?
5'-TMP + H2O
thymidine + phosphate
-
-
-
-
?
5'-TMP + H2O
thymidine + phosphate
-
107% of the activity of ATP
-
-
?
5'-TMP + H2O
thymidine + phosphate
-
-
-
?
5'-UMP + H2O
uridine + phosphate
-
plasma membrane enzyme: 7.5% of the activity with 5'-AMP, Golgi apparatus enzyme: 16.2% of the activity with 5'-AMP
-
?
5'-UMP + H2O
uridine + phosphate
-
10.6% of the activity with 5'-AMP
-
?
5'-UMP + H2O
uridine + phosphate
Bacillus sp. (in: Bacteria) No. A-59
-
10.6% of the activity with 5'-AMP
-
?
5'-UMP + H2O
uridine + phosphate
low activity
-
-
?
5'-UMP + H2O
uridine + phosphate
low activity
-
-
?
5'-UMP + H2O
uridine + phosphate
-
-
-
?
5'-UMP + H2O
uridine + phosphate
-
-
-
?
5'-UMP + H2O
uridine + phosphate
-
117% of the activity with 5'-AMP
-
?
5'-UMP + H2O
uridine + phosphate
-
-
-
?
5'-UMP + H2O
uridine + phosphate
-
49.5% of the activity with 5'-AMP
-
?
5'-UMP + H2O
uridine + phosphate
-
41% of the activity with 5-IMP
-
-
?
5'-UMP + H2O
uridine + phosphate
-
97% of activity with Mg2+-AMP
-
-
?
5'-UMP + H2O
uridine + phosphate
-
120% of the activity of 5'-IMP
-
?
5'-UMP + H2O
uridine + phosphate
-
-
-
?
5'-UMP + H2O
uridine + phosphate
-
-
-
?
5'-UMP + H2O
uridine + phosphate
-
-
-
?
5'-UMP + H2O
uridine + phosphate
-
-
-
-
?
5'-UMP + H2O
uridine + phosphate
about 30% of the activity with 4-methylumbelliferyl phosphate
-
?
5'-UMP + H2O
uridine + phosphate
-
-
-
?
5'-UMP + H2O
uridine + phosphate
-
-
-
?
5'-UMP + H2O
uridine + phosphate
-
-
-
-
?
5'-UMP + H2O
uridine + phosphate
-
-
-
?
5'-UMP + H2O
uridine + phosphate
-
-
-
?
5'-UMP + H2O
uridine + phosphate
-
14% of the activity with 5'-IMP, cytoplasmic enzyme
-
?
5'-UMP + H2O
uridine + phosphate
78% of the activity with 5'-AMP
-
?
5'-UMP + H2O
uridine + phosphate
-
CD73
-
-
?
5'-UMP + H2O
uridine + phosphate
-
33.3% of the activity with 5'-AMP
-
?
5'-UMP + H2O
uridine + phosphate
-
-
-
?
5'-UMP + H2O
uridine + phosphate
-
-
-
?
5'-UMP + H2O
uridine + phosphate
-
180% of the activity with 5'-AMP, preferred substrate
-
?
5'-UMP + H2O
uridine + phosphate
-
-
-
?
5'-UMP + H2O
uridine + phosphate
-
52.3% of the activity with 5'-IMP
-
?
5'-UMP + H2O
uridine + phosphate
-
-
-
?
5'-UMP + H2O
uridine + phosphate
-
-
-
?
5'-UMP + H2O
uridine + phosphate
-
-
-
?
5'-UMP + H2O
uridine + phosphate
-
-
-
?
5'-UMP + H2O
uridine + phosphate
-
-
-
?
5'-UMP + H2O
uridine + phosphate
-
-
-
?
5'-UMP + H2O
uridine + phosphate
-
93% of the activity with 5'-AMP, heart, membrane-bound enzyme
-
?
5'-UMP + H2O
uridine + phosphate
-
111% of the activity with 5'-AMP, brain cytosolic enzyme
-
?
5'-UMP + H2O
uridine + phosphate
-
51% dephosphorylation in relation to AMP
-
-
?
5'-UMP + H2O
uridine + phosphate
-
-
-
?
5'-UMP + H2O
uridine + phosphate
-
Sdt1
-
-
?
5'-UMP + H2O
uridine + phosphate
-
83% of the activity of ATP
-
?
5'-UMP + H2O
uridine + phosphate
-
56% of the activity with 5-IMP
-
-
?
5'-UMP + H2O
uridine + phosphate
-
-
-
?
5'-UMP + H2O
uridine + phosphate
-
-
-
?
5'-UMP + H2O
uridine + phosphate
-
-
-
?
5'-UMP + H2O
uridine + phosphate
-
14% of the activity with 5'-IMP
-
?
5'-UMP + H2O
uridine + phosphate
-
80% of the activity with 5'-AMP
-
?
5'-UMP + H2O
uridine + phosphate
-
60% activity compared to 5'-AMP
-
-
?
5'-UMP + H2O
uridine + phosphate
-
60% activity compared to 5'-AMP
-
-
?
5'-UMP + H2O
uridine + phosphate
-
33% of the activity with 5-IMP
-
-
?
5'-UMP + H2O
uridine + phosphate
-
-
-
?
5'-UMP + H2O
uridine + phosphate
-
71% of the activity with 5'-AMP
-
?
5'-UMP + H2O
uridine + phosphate
-
137% of the activity with 5'-AMP
-
?
5'-XMP + H2O
xanthosine + phosphate
third best substrate
-
-
?
5'-XMP + H2O
xanthosine + phosphate
-
-
-
?
5'-XMP + H2O
xanthosine + phosphate
-
-
-
?
5'-XMP + H2O
xanthosine + phosphate
-
-
-
-
?
5'-XMP + H2O
xanthosine + phosphate
-
23% of the activity with 5'-IMP, cytoplasmic enzyme
-
?
5'-XMP + H2O
xanthosine + phosphate
-
cNII, CD73
-
-
?
5'-XMP + H2O
xanthosine + phosphate
-
-
-
?
a 5'-ribonucleotide + H2O
a ribonucleoside + phosphate
-
-
-
?
a 5'-ribonucleotide + H2O
a ribonucleoside + phosphate
-
-
-
?
a 5'-ribonucleotide + H2O
a ribonucleoside + phosphate
-
-
-
-
?
a 5'-ribonucleotide + H2O
a ribonucleoside + phosphate
-
-
-
?
a 5'-ribonucleotide + H2O
a ribonucleoside + phosphate
-
-
-
?
ADP + 2 H2O
adenosine + 2 phosphate
-
-
-
-
?
ADP + 2 H2O
adenosine + 2 phosphate
-
-
-
-
?
ADP + 2 H2O
adenosine + 2 phosphate
-
-
-
-
?
ADP + 2 H2O
adenosine + 2 phosphate
-
-
-
-
?
ADP + 2 H2O
adenosine + 2 phosphate
-
114% of the activity of ATP
-
-
?
ADP + 2 H2O
adenosine + 2 phosphate
-
-
-
-
?
ADP + 2 H2O
adenosine + 2 phosphate
-
-
-
-
?
ADP + 2 H2O
adenosine + 2 phosphate
-
-
-
-
?
ADP + 2 H2O
adenosine + 2 phosphate
-
-
-
?
ADP + H2O
5'-AMP + phosphate
-
-
-
?
ADP + H2O
5'-AMP + phosphate
-
-
-
?
ADP + H2O
adenosine + diphosphate
-
9.6% of the activity with 5'-AMP
-
?
ADP + H2O
adenosine + diphosphate
-
-
-
?
ADP + H2O
adenosine + diphosphate
-
-
-
?
ADP + H2O
adenosine + diphosphate
-
4.5% of the activity with 5'-AMP
-
?
AMP + H2O
adenosine + phosphate
-
-
-
-
?
AMP + H2O
adenosine + phosphate
-
AMP in form of MgAMP
-
-
?
AMP + H2O
adenosine + phosphate
-
-
-
?
AMP + H2O
adenosine + phosphate
-
-
-
?
AMP + H2O
adenosine + phosphate
-
-
-
-
?
AMP + H2O
adenosine + phosphate
-
-
-
-
?
AMP + H2O
adenosine + phosphate
-
-
-
-
?
ATP + 3 H2O
adenosine + 3 phosphate
-
-
-
-
?
ATP + 3 H2O
adenosine + 3 phosphate
-
-
-
-
?
ATP + 3 H2O
adenosine + 3 phosphate
-
-
-
-
?
ATP + 3 H2O
adenosine + 3 phosphate
-
-
-
-
?
ATP + 3 H2O
adenosine + 3 phosphate
-
-
-
-
?
ATP + 3 H2O
adenosine + 3 phosphate
-
-
-
-
?
ATP + 3 H2O
adenosine + 3 phosphate
-
-
-
-
?
ATP + 3 H2O
adenosine + 3 phosphate
-
-
-
-
?
ATP + 3 H2O
adenosine + 3 phosphate
-
-
-
?
ATP + H2O
ADP + phosphate
-
-
-
-
?
ATP + H2O
ADP + phosphate
lowest activity
-
-
?
D-ribose 5-phosphate + H2O
D-ribose + phosphate
second best substrate
-
-
?
D-ribose 5-phosphate + H2O
D-ribose + phosphate
second best substrate
-
-
?
dCMP + H2O
deoxycytidine + phosphate
-
-
-
?
dCMP + H2O
deoxycytidine + phosphate
10% of the activity with 5'-AMP
-
?
dIMP + H2O
deoxyinosine + phosphate
84% of the activity with 5'-AMP
-
?
dIMP + H2O
deoxyinosine + phosphate
-
103% of the activity with 5'-IMP
-
?
diphosphate + H2O
2 phosphate
-
5.8% activity compared to 5'-AMP
-
-
?
diphosphate + H2O
2 phosphate
-
5.8% activity compared to 5'-AMP
-
-
?
inosine + phosphate
5'-IMP + H2O
-
-
-
-
r
inosine + phosphate
5'-IMP + H2O
-
-
-
r
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
-
-
?
additional information
?
-
no activity with 3'-CMP, 3'-AMP, and UDP-D-glucose
-
-
?
additional information
?
-
-
no activity with 3'-CMP, 3'-AMP, and UDP-D-glucose
-
-
?
additional information
?
-
no activity with 3'-CMP, 3'-AMP, and UDP-D-glucose
-
-
?
additional information
?
-
-
significant differences in substrate specificity between the isoforms are interpreted in terms of variable glycosylation causing steric hindrance of the substrate-binding site
-
?
additional information
?
-
-
the enzyme acts through the formation of a phosphoenzyme intermediate, essential role of Asp52 in the catalytic machinery, Asp54 assists in the formation of the acyl phosphate species
-
?
additional information
?
-
-
the soluble forms of enzyme in seminal plasma have a putative role in signalling interactions with spermatozoa following ejaculation and capacitations in the female reproductive tract
-
?
additional information
?
-
-
no substrate: 3'-IMP, 1-glycerophosphate
-
-
?
additional information
?
-
-
bifunctional enzyme with UDP-sugar hydrolase and 5'-nucleotidase activities
-
?
additional information
?
-
-
5'-nucleotidase catalyzes the hydrolysis of the phosphoric ester bond of nucleoside 5'-monophosphates, such as AMP, CMP, UMP, IMP and GMP, to the corresponding nucleosides and phosphate
-
-
?
additional information
?
-
alcohols do not function as phosphate acceptors and suggests that the enzyme lacks transphosphorylation activity
-
?
additional information
?
-
-
alcohols do not function as phosphate acceptors and suggests that the enzyme lacks transphosphorylation activity
-
?
additional information
?
-
no dephosphorylation of araCMP and 2-fluoroadenine-9-beta-D-arabinofuranoside
-
?
additional information
?
-
-
no dephosphorylation of araCMP and 2-fluoroadenine-9-beta-D-arabinofuranoside
-
?
additional information
?
-
cN-I may play an important role in the regulation of physiological pyrimidine nucleotide pools and may also alter the therapeutic efficiacy of certain nucleoside analogs
-
?
additional information
?
-
-
cN-I may play an important role in the regulation of physiological pyrimidine nucleotide pools and may also alter the therapeutic efficiacy of certain nucleoside analogs
-
?
additional information
?
-
-
major role in regulation of adenosine concentrations on airway surfaces
-
?
additional information
?
-
-
patients with nucleotidase-associated pervasive developmental disorder exhibit altered electrophoretic mobility of cytosolic 5'-nucleotidase
-
?
additional information
?
-
-
the enzyme contributes to function of endothelial cells by inhibiting platelet aggregation in cooperation with ATP diphosphohydrolase, which degrades ADP to AMP
-
?
additional information
?
-
cytosolic 5'-nucleotidase II catalyzes the dephosphorylation of 6-hydroxypurine nucleoside 5'-monophosphates
-
-
?
additional information
?
-
-
cytosolic 5'-nucleotidase II catalyzes the dephosphorylation of 6-hydroxypurine nucleoside 5'-monophosphates
-
-
?
additional information
?
-
-
Usha, Pho5, Sdt1 and Phm8 are phosphate starvation-induced 5'-nucleotidases with diverse substrate specificities that liberate phosphate under phosphate starvation conditions
-
-
?
additional information
?
-
structural basis for the substrate specificity of cN-II: Arg202, Asp206, and Phe157 seem to be important residues for purine/pyrimidine selectivity
-
-
?
additional information
?
-
-
structural basis for the substrate specificity of cN-II: Arg202, Asp206, and Phe157 seem to be important residues for purine/pyrimidine selectivity
-
-
?
additional information
?
-
poor substrate: beta-nicotinamide mononucleotide
-
-
?
additional information
?
-
-
poor substrate: beta-nicotinamide mononucleotide
-
-
?
additional information
?
-
activity on AMP, XMP, CMP, UMP and -nitrophenyl phosphate is poor
-
-
?
additional information
?
-
activity on AMP, XMP, CMP, UMP and -nitrophenyl phosphate is poor
-
-
?
additional information
?
-
-
5'-nucleotidase and phosphodiesterase reside in the same molecule
-
?
additional information
?
-
no activity with 3'-AMP, 3',5'-cAMP, and 3',5'-cGMP
-
-
?
additional information
?
-
-
no activity with 3'-AMP, 3',5'-cAMP, and 3',5'-cGMP
-
-
?
additional information
?
-
-
accumulation of Arg, N-acetylarginine, homoarginine and argininic acid do not alter 5'-nucleotidase activity
-
?
additional information
?
-
-
involved in the synthesis of guanine nucleotides from xanthosine
-
-
?
additional information
?
-
-
IMP and GMP are the best phosphate donors for phosphotransferase activity, followed by UMP and CMP
-
-
?
additional information
?
-
-
the enzyme plays a role on purine salvage pathways
-
?
additional information
?
-
-
Sdt1 is highly specific for 5'-UMP and 5'-CMP, and shows negligible activity on purine nucleoside monophosphates, 3'-monophosphates or nucleoside triphosphates
-
-
?
additional information
?
-
-
no substrate: 3'-IMP, 1-glycerophosphate
-
-
?
additional information
?
-
no activity with ATP
-
-
?
additional information
?
-
-
no activity with ATP
-
-
?
additional information
?
-
no activity with ATP
-
-
?
additional information
?
-
-
high enzyme activity in liver is a feature of constitutive uricotelism, enzyme may participate in the production of uric acid
-
-
?
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(2E)-5-(5-methylfuran-2-yl)-N-(2-methylphenyl)-3-oxo-2-(2-oxo-1,2-dihydro-3H-indol-3-ylidene)-2,3-dihydro-5H-[1,3]thiazolo[3,2-a]pyrimidine-6-carboxamide
67% inhibition at 0.005 mM
(2R,4S,5R)-1-[5-(2-fluoro-2-phosphonoethyl)tetrahydro-4-hydroxy-2-furyl]thymine
-
-
(2R,4S,5R)-1-[tetrahydro-4-hydroxy-5-(2-phosphonoethyl)-2-furyl]thymine
-
-
(2R,5R)-1-[5-(2-fluoro-2-phosphonoethyl)tetrahydro-2-furyl]thymine
-
-
(2R,5S)-1-[tetrahydro-5-(2-phosphonoethyl)-2-furyl]thymine
-
-
(2Z)-2-[[3-(3-fluoro-4-methoxyphenyl)-1-phenyl-1H-pyrazol-4-yl]methylidene]-6-[(4-methylphenyl)methyl]-7H-[1,3]thiazolo[3,2-b][1,2,4]triazine-3,7(2H)-dione
61% inhibition at 0.005 mM
1,10-phenanthroline
-
inhibition of cytosolic and membrane-bound enzyme
1-(6-deoxy-6-phosphono-beta-D-allofuranosyl)-5-(2-(E)-phenylvinyl)-cytosine
-
1-(6-deoxy-6-phosphono-beta-D-allofuranosyl)-5-phenyl-cytosine
-
1-(6-deoxy-6-phosphono-beta-D-allofuranosyl)-cytosine
-
1-amino-2-methyl-4-(phenylamino)anthracene-9,10-dione
-
4% inhibition at 1 mM
1-amino-4-(1-anthracenylamino)-9,10-dioxo-9,10 dihydroanthracene-2-sulfonate
-
-
1-amino-4-(1-naphthylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
competitive mechanism
1-amino-4-(2,3-dimethylphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
39% inhibition at 1 mM
1-amino-4-(2,4-dimethylphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
47% inhibition at 1 mM
1-amino-4-(2,5-dimethylphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
26% inhibition at 1 mM
1-amino-4-(2-aminophenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
47% inhibition at 1 mM
1-amino-4-(2-anthracenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
competitive mechanism
1-amino-4-(2-carboxy-4-chlorophenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
52% inhibition at 1 mM
1-amino-4-(2-carboxy-4-fluorophenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
1-amino-4-(2-carboxy-5-chlorophenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
27% inhibition at 1 mM
1-amino-4-(2-carboxy-5-fluorophenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
60% inhibition at 1 mM
1-amino-4-(2-carboxyphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
1-amino-4-(2-ethoxyphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
56% inhibition at 1 mM
1-amino-4-(2-hydroxyphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
1-amino-4-(2-methoxyphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
9% inhibition at 1 mM
1-amino-4-(2-methyl-1-naphthylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
1-amino-4-(2-methylphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
33% inhibition at 1 mM
1-amino-4-(2-naphthylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
1-amino-4-(2-sulfophenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
14% inhibition at 1 mM
1-amino-4-(3,4-dimethoxyphenethylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
44% inhibition at 1 mM
1-amino-4-(3-amino-2,4,6-trimethylphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
1-amino-4-(3-amino-4-sulfophenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
1-amino-4-(3-amino-5-carboxyphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
44% inhibition at 1 mM
1-amino-4-(3-aminophenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
31% inhibition at 1 mM
1-amino-4-(3-carboxyphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
26% inhibition at 1 mM
1-amino-4-(3-methylphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
1-amino-4-(4-amino-3-sulfophenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
1-amino-4-(4-aminophenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
1-amino-4-(4-bromo-2-carboxyphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
56% inhibition at 1 mM
1-amino-4-(4-bromophenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
1-amino-4-(4-carboxymethylphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
26% inhibition at 1 mM
1-amino-4-(4-chlorophenethylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
62% inhibition at 1 mM
1-amino-4-(4-chlorophenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
1-amino-4-(4-ethoxyphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
31% inhibition at 1 mM
1-amino-4-(4-fluorophenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
1-amino-4-(4-hydroxyphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
1-amino-4-(4-methylphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
1-amino-4-(4-phenoxyphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
1-amino-4-(4-phenylaminophenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
37% inhibition at 1 mM
1-amino-4-(4-sulfophenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
1-amino-4-(5-sulfo-1-naphthylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
1-amino-4-(6-carboxy-2-naphthylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
1-amino-4-(6-sulfo-1-naphthylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
1-amino-4-(8-sulfo-1-naphthylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
1-amino-4-(9-phenanthrenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
58% inhibition at 1 mM
1-amino-4-(benzylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
43% inhibition at 1 mM
1-amino-4-(cyclohexylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
1-amino-4-(cyclopentylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
51% inhibition at 1 mM
1-amino-4-(isopropylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
15% inhibition at 1 mM
1-amino-4-(phenethylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
53% inhibition at 1 mM
1-amino-4-bromo-9,10-dioxo-9,10-dihydroanthracene-2-sulfonic acid
1-amino-4-[4-(diethoxyphosphoryl)methylphenylamino]-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
28% inhibition at 1 mM
1-pentyl-1H-benzofuro[3,2-b]pyrrolo[3,2-e]pyridine-3-carbonitrile
1-tert-butyl-1H-benzofuro[3,2-b]pyrrolo[3,2-e]pyridine-3-carbonitrile
1-tert-butyl-5-chloro-6-(2-hydroxy-5-methylphenyl)-1H-pyrrolo[2,3-b]pyridine-3-carbonitrile
1-tert-butyl-5-chloro-6-(2-hydroxyphenyl)-1H-pyrrolo[2,3-b]pyridine-3-carbonitrile
2,2'-(2-(2-((2S,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-carboxamido)ethylamino)-2-oxoethylazanediyl) diacetic acid
nucleotide-derived inhibitor
2,3,5-tri-O-benzyl-1-O-(4-nitrobenzoyl)-D-arabinofuranose
68% inhibition at 0.005 mM
2-((4'-cyano-4,4''-dimethoxy-[1,1':3',1''-terphenyl]-5'-yl)thio)-N-(3-cyano-4,5,6,7-tetrahydrobenzo[b]thiophen-2-yl)acetamide
73% inhibition at 0.005 mM
2-(3,4-dihydroisoquinolin-2(1H)-yl)-N-[4-[1-(4-methylbenzoyl)-2,3-dihydro-1H-indol-5-yl]-5-sulfanyl-1,3-thiazol-2-yl]acetamide
74% inhibition at 0.005 mM
2-(3-methyl-1,5-diphenyl-1H-pyrazolo[3,4-b]pyridin-6-yl)phenol
2-(5-chloro-1,3-dimethyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-4-methylphenol
2-(5-chloro-1,3-dimethyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-5-methoxyphenol
2-(5-chloro-1,3-dimethyl-1H-pyrazolo[3,4-b]pyridin-6-yl)phenol
2-(5-chloro-3-methyl-1-p-tolyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-4-methylphenol
2-(5-chloro-3-methyl-1-p-tolyl-1H-pyrazolo[3,4-b]pyridin-6-yl)phenol
2-(5-chloro-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-4-methylphenol
2-(5-chloro-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-5-methoxyphenol
2-(5-chloro-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridin-6-yl)phenol
2-chloro-N-(pyridin-4-yl)benzamide
-
2-fluoroadenine-9-beta-D-arabinofuranoside
1 mM, 70% inhibition with 5 mM 5'-AMP as substrate
2-methyl-N-(pyridin-4-yl)benzamide
-
2-nitro-N-(pyridin-4-yl)benzamide
-
2-phenyl-N-(pyridin-4-yl)acetamide
-
3,4,5-trimethoxy-N-(pyridin-4-yl)benzamide
-
3,5-dinitro-N-(pyridine-4-yl)benzamide
-
3,8-dimethyl-1-p-tolyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine
3,8-dimethyl-1-phenyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine
3-(2-(3,4,5-trimethoxybenzylideneamino)thiazol-4-yl)-2Hchromen-2-one
3-(2-(3-chlorobenzylideneamino)thiazol-4-yl)-2H-chromen-2-one
3-(2-(3-methoxy-4-phenoxybenzylideneamino)thiazol-4-yl)-2H-chromen-2-one
3-(2-(3-nitrobenzylideneamino)thiazol-4-yl)-2H-chromen-2-one
3-(2-(4-bromobenzylideneamino)thiazol-4-yl)-2H-chromen-2-one
3-(2-(4-chlorobenzylideneamino)thiazol-4-yl)-2H-chromen-2-one
3-(2-(4-fluorobenzylideneamino)thiazol-4-yl)-2H-chromen-2-one
3-(2-(furan-2-ylmethyleneamino)thiazol-4-yl)-2H-chromen-2-one
3-chloro-N-(pyridin-4-yl)benzamide
-
3-methyl-1-p-tolyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine
3-methyl-1-phenyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine
3-methyl-N-(pyridin-4-yl)benzamide
-
3-nitro-N-(pyridin-4-yl)benzamide
-
3-[4,5-di([1,1'-biphenyl]-4-yl)-1H-imidazol-2-yl]-1H-indole
93% inhibition at 0.005 mM
4-(3-methyl-5-oxo-4,5-dihydro-1H-pyrazol-1-yl)benzenesulfonic acid
4-(4-[1-[(3-chlorophenyl)methyl]-4-hydroxy-1H-[1,2,3]triazolo[4,5-c]pyridin-6-yl]piperidine-1-sulfonyl)-N-cycloheptylbenzene-1-sulfonamide
63% inhibition at 0.005 mM
4-(6-[[2-(4-aminophenyl)-1H-benzimidazol-5-yl]methyl]-1H-benzimidazol-2-yl)aniline
78% inhibition at 0.005 mM
4-chloro-N-(pyridin-4-yl)benzamide
-
4-methyl-N-(pyridin-4-yl)benzamide
-
4-nitro-N-(pyridin-4-yl)benzamide
-
4-nitrophenyl phosphate
-
-
5'-deoxy-5'-S-isobutylthioadenosine
-
-
5'-deoxy-7-methyl-5'-(4-phosphono-1H-pyrazol-1-yl)guanosine
-
moderately potent inhibitor
5-chloro-3-methyl-1-phenyl-6-(2-propoxyphenyl)-1H-pyrazolo[3,4-b]pyridine
5-chloro-6-(2-(heptyloxy)phenyl)-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridine
5-chloro-6-(2-hydroxy-5-methylphenyl)-2-phenyl-2H-pyrazolo[3,4-b]pyridin-3-ol
5-chloro-6-(2-hydroxyphenyl)-2-phenyl-2H-pyrazolo[3,4-b]pyridin-3-ol
5-chloro-6-(2-methoxyphenyl)-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridine
5-ethynyl-2',3'-dideoxyuridine
-
-
5-ethynyldideoxyuridine
-
selective inhibitor
6-(4-ethoxyphenyl)-3-(4-methylphenyl)-N-(2-phenylethyl)-1H-pyrazolo[3,4-b]pyridine-4-carboxamide
65% inhibition at 0.005 mM
6-amino-4-hydroxynaphthalene-2-sulfonic acid
6-chloro-7-(2-hydroxy-5-methylphenyl)pyrido[2,3-d]pyrimidine-2,4(1H,3H)-dione
6-chloro-7-(2-hydroxyphenyl)-1-methylpyrido[2,3-d]pyrimidine-2,4(1H,3H)-dione
6-chloro-7-(2-hydroxyphenyl)pyrido[2,3-d]pyrimidine-2,4(1H,3H)-dione
6-Chloropurine riboside
-
-
6-gamma,gamma-dimethylallylaminopurine riboside
-
-
6-mercaptopurine riboside
-
-
6-N,N-diethyl-D-beta-gamma-dibromomethylene adenosine triphosphate
originally named FPL 67156 or ARL 67156, 28% inhibition at 0.1 mM
7-methoxy-3-methyl-1-phenyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine
adenosine 5'-(alpha,beta-methylene)diphosphate
adenosine 5'-(alpha,beta-methylene)triphosphate
-
-
alpha,beta-methylene adenosine 5'-diphosphate
-
specific inhibitor
alpha,beta-methylene adenosine diphosphate
-
0.1 mM
alpha,beta-methylene adenosine-5'-diphosphate
-
-
alpha,beta-methylene ADP
-
-
alpha-beta-methylene-ADP
-
alphabeta-methylene ADP
-
0.05 mM
Ap[CH2]p
-
mixed competitive inhibitor
araCMP
1 mM, 25% inhibition with 5 mM 5'-AMP as substrate
benzoic acid
-
inhibition of cytosolic enzyme
beta-gamma-methylene-ATP
-
clomipramine
-
enzymatic activity is decreased by the antidepressant clomipramine after in vitro exposure
DTT
1 mM, about 45% inhibition
ethanol
-
23% and 28% inhibition at 0.5% and 1% (v/v) ethanol, respectively
ethyl 4-(2-((4'-cyano-4''-methoxy-4-methyl-[1,1':3',1''-terphenyl]-5'-yl)thio)acetamido)benzoate
80% inhibition at 0.005 mM
ethyl 4-(3-hydroxyphenyl)-6-methyl-2-sulfanylidene-1,2,3,4-tetrahydropyrimidine-5-carboxylate
monastrol
ethyl 4-[4-(dimethylamino)phenyl]-6-methyl-2-sulfanylidene-1,2,3,4-tetrahydropyrimidine-5-carboxylate
LaSOM 63
gamma-glutamylcysteine
-
-
Inosine
-
strong inhibition, enzymes N-I and N-II
L-arginine
-
21% inhibition at 0.1 mM
L-ascorbic acid
-
inhibition of cytosolic enzyme
L-cysteine ethyl ester
-
-
levamisole
-
1 mM, 81% residual activity
Ly-294002
-
0.01 mM LY-294002 abolishes lipopolysaccharide-induced ecto-5'-nucleotidase activity
MEDI9447
non-competitive inhibitor
-
molybdate
1 mM, about 15% inhibition
N-(4-(4-methoxyphenyl)thiazol-2-yl)acrylamide
-
selective inhibitor
N-(4-methylcyclohexyl)-1-(2-phenyl-1H-benzimidazole-5-sulfonyl)piperidine-3-carboxamide
17% inhibition at 0.005 mM
N-(pyridin-4-yl)furan-2-carboxamide
-
N-[4-[5-([2-[2-(4-methoxybenzoyl)hydrazinyl]-2-oxoethyl]sulfanyl)-4-(4-methoxyphenyl)-4H-1,2,4-triazol-3-yl]phenyl]-4-methylbenzene-1-sulfonamide
64% inhibition at 0.005 mM
p-chloromercuribenzoate
-
inhibition of cytosolic and membrane-bound enzyme
phenylmethylsulfonyl fluoride
-
inhibition of cytosolic enzyme
PMSF
1 mM, about 40% inhibition
sertraline
-
enzymatic activity is decreased by the antidepressant sertraline after in vitro exposure
sodium 1-amino-4-(2-carboxy-4-fluoroanilino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
PSB-0952
sodium 1-amino-4-anilino-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
AB-25
sodium 1-amino-4-[(anthracen-2-yl)amino]-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
PSB-0963
sodium 1-amino-9,10-dioxo-4-(phenylamino)-9,10-dihydroanthracene-2-sulfonate
-
-
Specific protein inhibitor
-
the periplasmic enzyme has a specific protein inhibitor located in the cytoplasm. The physiological role of the inhibitor is to protect the intracellular nucleotide pool from any cytoplasmic 5'-nucleotidase activity
-
Tartrate
1 mM, about 50% inhibition
tetramisole
-
1 mM, 95% residual activity
tetrasodium 4-[[4-([4-[(4-amino-9,10-dioxo-3-sulfonato-9,10-dihydroanthracen-1-yl)amino]-2-sulfonatophenyl]amino)-6-chloro-1,3,5-triazin-2-yl]amino]benzene-1,2-disulfonate
-
-
thio-IMP
-
substrate inhibition
vanadate
1 mM, about 15% inhibition
1-amino-4-bromo-9,10-dioxo-9,10-dihydroanthracene-2-sulfonic acid
-
-
1-amino-4-bromo-9,10-dioxo-9,10-dihydroanthracene-2-sulfonic acid
-
-
1-pentyl-1H-benzofuro[3,2-b]pyrrolo[3,2-e]pyridine-3-carbonitrile
-
1-pentyl-1H-benzofuro[3,2-b]pyrrolo[3,2-e]pyridine-3-carbonitrile
-
1-tert-butyl-1H-benzofuro[3,2-b]pyrrolo[3,2-e]pyridine-3-carbonitrile
-
1-tert-butyl-1H-benzofuro[3,2-b]pyrrolo[3,2-e]pyridine-3-carbonitrile
-
1-tert-butyl-5-chloro-6-(2-hydroxy-5-methylphenyl)-1H-pyrrolo[2,3-b]pyridine-3-carbonitrile
-
1-tert-butyl-5-chloro-6-(2-hydroxy-5-methylphenyl)-1H-pyrrolo[2,3-b]pyridine-3-carbonitrile
-
1-tert-butyl-5-chloro-6-(2-hydroxyphenyl)-1H-pyrrolo[2,3-b]pyridine-3-carbonitrile
-
1-tert-butyl-5-chloro-6-(2-hydroxyphenyl)-1H-pyrrolo[2,3-b]pyridine-3-carbonitrile
-
2-(3-methyl-1,5-diphenyl-1H-pyrazolo[3,4-b]pyridin-6-yl)phenol
-
2-(3-methyl-1,5-diphenyl-1H-pyrazolo[3,4-b]pyridin-6-yl)phenol
-
2-(5-chloro-1,3-dimethyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-4-methylphenol
-
2-(5-chloro-1,3-dimethyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-4-methylphenol
-
2-(5-chloro-1,3-dimethyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-5-methoxyphenol
-
2-(5-chloro-1,3-dimethyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-5-methoxyphenol
-
2-(5-chloro-1,3-dimethyl-1H-pyrazolo[3,4-b]pyridin-6-yl)phenol
-
2-(5-chloro-1,3-dimethyl-1H-pyrazolo[3,4-b]pyridin-6-yl)phenol
-
2-(5-chloro-3-methyl-1-p-tolyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-4-methylphenol
-
2-(5-chloro-3-methyl-1-p-tolyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-4-methylphenol
-
2-(5-chloro-3-methyl-1-p-tolyl-1H-pyrazolo[3,4-b]pyridin-6-yl)phenol
-
2-(5-chloro-3-methyl-1-p-tolyl-1H-pyrazolo[3,4-b]pyridin-6-yl)phenol
-
2-(5-chloro-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-4-methylphenol
-
2-(5-chloro-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-4-methylphenol
-
2-(5-chloro-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-5-methoxyphenol
-
2-(5-chloro-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-5-methoxyphenol
-
2-(5-chloro-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridin-6-yl)phenol
-
2-(5-chloro-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridin-6-yl)phenol
-
3,8-dimethyl-1-p-tolyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine
-
3,8-dimethyl-1-p-tolyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine
-
3,8-dimethyl-1-phenyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine
-
3,8-dimethyl-1-phenyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine
-
3-(2-(3,4,5-trimethoxybenzylideneamino)thiazol-4-yl)-2Hchromen-2-one
-
-
3-(2-(3,4,5-trimethoxybenzylideneamino)thiazol-4-yl)-2Hchromen-2-one
-
-
3-(2-(3-chlorobenzylideneamino)thiazol-4-yl)-2H-chromen-2-one
-
-
3-(2-(3-chlorobenzylideneamino)thiazol-4-yl)-2H-chromen-2-one
-
-
3-(2-(3-methoxy-4-phenoxybenzylideneamino)thiazol-4-yl)-2H-chromen-2-one
-
-
3-(2-(3-methoxy-4-phenoxybenzylideneamino)thiazol-4-yl)-2H-chromen-2-one
-
-
3-(2-(3-nitrobenzylideneamino)thiazol-4-yl)-2H-chromen-2-one
-
-
3-(2-(3-nitrobenzylideneamino)thiazol-4-yl)-2H-chromen-2-one
-
-
3-(2-(4-bromobenzylideneamino)thiazol-4-yl)-2H-chromen-2-one
-
-
3-(2-(4-bromobenzylideneamino)thiazol-4-yl)-2H-chromen-2-one
-
-
3-(2-(4-chlorobenzylideneamino)thiazol-4-yl)-2H-chromen-2-one
-
-
3-(2-(4-chlorobenzylideneamino)thiazol-4-yl)-2H-chromen-2-one
-
-
3-(2-(4-fluorobenzylideneamino)thiazol-4-yl)-2H-chromen-2-one
-
-
3-(2-(4-fluorobenzylideneamino)thiazol-4-yl)-2H-chromen-2-one
-
-
3-(2-(furan-2-ylmethyleneamino)thiazol-4-yl)-2H-chromen-2-one
-
-
3-(2-(furan-2-ylmethyleneamino)thiazol-4-yl)-2H-chromen-2-one
-
-
3-methyl-1-p-tolyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine
-
3-methyl-1-p-tolyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine
-
3-methyl-1-phenyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine
-
3-methyl-1-phenyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine
-
4-(3-methyl-5-oxo-4,5-dihydro-1H-pyrazol-1-yl)benzenesulfonic acid
-
compound shows high cytotoxicity on H157 cells, with about 72% at 100 mM final concentration
4-(3-methyl-5-oxo-4,5-dihydro-1H-pyrazol-1-yl)benzenesulfonic acid
-
-
5-chloro-3-methyl-1-phenyl-6-(2-propoxyphenyl)-1H-pyrazolo[3,4-b]pyridine
-
5-chloro-3-methyl-1-phenyl-6-(2-propoxyphenyl)-1H-pyrazolo[3,4-b]pyridine
-
5-chloro-6-(2-(heptyloxy)phenyl)-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridine
-
5-chloro-6-(2-(heptyloxy)phenyl)-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridine
-
5-chloro-6-(2-hydroxy-5-methylphenyl)-2-phenyl-2H-pyrazolo[3,4-b]pyridin-3-ol
-
5-chloro-6-(2-hydroxy-5-methylphenyl)-2-phenyl-2H-pyrazolo[3,4-b]pyridin-3-ol
-
5-chloro-6-(2-hydroxyphenyl)-2-phenyl-2H-pyrazolo[3,4-b]pyridin-3-ol
-
5-chloro-6-(2-hydroxyphenyl)-2-phenyl-2H-pyrazolo[3,4-b]pyridin-3-ol
-
5-chloro-6-(2-methoxyphenyl)-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridine
-
5-chloro-6-(2-methoxyphenyl)-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridine
-
6-amino-4-hydroxynaphthalene-2-sulfonic acid
-
most potent inhibitor of the screen
6-amino-4-hydroxynaphthalene-2-sulfonic acid
-
most potent inhibitor of the screen
6-chloro-7-(2-hydroxy-5-methylphenyl)pyrido[2,3-d]pyrimidine-2,4(1H,3H)-dione
-
6-chloro-7-(2-hydroxy-5-methylphenyl)pyrido[2,3-d]pyrimidine-2,4(1H,3H)-dione
-
6-chloro-7-(2-hydroxyphenyl)-1-methylpyrido[2,3-d]pyrimidine-2,4(1H,3H)-dione
-
6-chloro-7-(2-hydroxyphenyl)-1-methylpyrido[2,3-d]pyrimidine-2,4(1H,3H)-dione
-
6-chloro-7-(2-hydroxyphenyl)pyrido[2,3-d]pyrimidine-2,4(1H,3H)-dione
-
6-chloro-7-(2-hydroxyphenyl)pyrido[2,3-d]pyrimidine-2,4(1H,3H)-dione
-
7-methoxy-3-methyl-1-phenyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine
-
7-methoxy-3-methyl-1-phenyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine
-
adenosine
-
strong inhibition, enzymes N-I and N-II
adenosine 5'-(alpha,beta-methylene)diphosphate
-
not inhibitory
adenosine 5'-(alpha,beta-methylene)diphosphate
-
specific inhibitor, complete inhibition at 0.5 mM
adenosine 5'-(alpha,beta-methylene)diphosphate
-
complete inhibition at 0.1 mM
adenosine 5'-(alpha,beta-methylene)diphosphate
-
competitive inhibitor
adenosine 5'-(alpha,beta-methylene)diphosphate
-
-
adenosine 5'-(alpha,beta-methylene)diphosphate
-
treatment with 0.001 mM adenosine 5'-(alpha,beta-methylene)diphosphate, a competitive ecto-5'-NT/CD73 inhibitor, causes a significant reduction of 30% in glioma cell proliferation, the inhibitory effect on cell proliferation caused by adenosine 5'-(alpha,beta-methylene)diphosphate is reverted by cotreatment with nitrobenzylthioinosine and dipyridamole
adenosine 5'-(alpha,beta-methylene)diphosphate
-
-
adenosine 5'-(alpha,beta-methylene)diphosphate
-
specific CD73 inhibitor
adenosine 5'-(alpha,beta-methylene)diphosphate
-
-
adenosine 5'-(alpha,beta-methylene)diphosphate
-
treatment with 0.001 mM adenosine 5'-(alpha,beta-methylene)diphosphate, a competitive ecto-5'-NT/CD73 inhibitor, causes a significant reduction of 30% in glioma cell proliferation, the inhibitory effect on cell proliferation caused by adenosine 5'-(alpha,beta-methylene)diphosphate is reverted by cotreatment with nitrobenzylthioinosine and dipyridamole
adenosine 5'-(alpha,beta-methylene)diphosphate
-
-
adenosine 5'-(alpha,beta-methylene)diphosphate
-
-
ADP
-
-
ADP
40% inhibition at 0.1 mM
ADP
-
competitive inhibitor
ADP
moderately potent inhibitor
ADP
competitive inhibitor
ADP
-
feed-forward inhibition
agglutinin
-
cytosolic enzyme
-
agglutinin
-
plasma membrane enzyme
-
agglutinin
-
enzyme from glioblastoma cells
-
alpha,beta-methylene-ADP
-
0.1 mM, 58% residual activity
alpha,beta-methylene-ADP
-
alpha,beta-methylene-ADP
-
-
alpha,beta-methylene-ADP
highly effective competitive inhibitor
alpha,beta-methylene-ADP
specific inhibitor
alpha,beta-methylene-ADP
-
specific inhibitor
alpha,beta-methylene-ADP
-
alpha,beta-methylene-ADP
competitive inhibitor
alpha,beta-methylene-ADP
-
-
alpha,beta-methylene-ADP
-
a specific CD73 inhibitor
alpha,beta-methylene-ADP
-
-
alpha,beta-methylene-ADP
-
alpha/beta-methylene-ADP
-
a specific CD73 inhibitor
alpha/beta-methylene-ADP
-
a specific CD73 inhibitor
Ammonium molybdate
-
-
Ammonium molybdate
-
17.6% residual activity at 0.1 mM ammonium molybdate
Ammonium molybdate
-
almost complete inhibition at 1.0 mM
ATP
-
-
ATP
-
inhibition at substrate concentration above 6 mM
ATP
-
inhibits eNs, no effect on cNI
ATP
70% inhibition at 0.1 mM
ATP
-
competitive inhibitor
ATP
competitive inhibitor
ATP
-
feed-forward inhibition
Ca2+
-
-
Ca2+
89.5% residual activity at 5 mM
Ca2+
-
gradual decrease in enzyme activity in the presence of 1-6 mM of Ca2+
Ca2+
5 mM, inhibition of both hydrolysis of AMP and ADP
Co2+
-
plasma membrane enzyme
Co2+
-
0.1 mM activate, 4 mM inhibit, plasma membrane enzyme
Co2+
-
strong, enzyme from glioblastoma cells
Co2+
-
inhibition of cytosolic and membrane-bound enzyme
concanavalin A
-
cytosolic enzyme
-
concanavalin A
-
plasma membrane enzyme
-
concanavalin A
-
strong inhibition at 0.02 mg/ml
-
concanavalin A
-
strong inhibitor
-
concanavalin A
-
upon blockade of enzyme by concanavalin A, the strength of adhesion to different extracellular matrix proteins is not altered, but at the same time the invasion ability of the cells is decreased
-
concanavalin A
-
partial non-competitive
-
concanavalin A
-
complete inhibition at 0.006 mM
-
concanavalin A
-
enzyme from glioblastoma cells
-
concanavalin A
-
strong inhibition
-
CTP
-
Cu2+
-
-
Cu2+
further inhibition of the residual activity in EDTA-treated enzyme
Cu2+
3.9% residual activity at 5 mM
Cu2+
-
inhibition of cytosolic and membrane-bound enzyme
dATP
-
dCTP
-
dGTP
-
EDTA
-
-
EDTA
-
plasma membrane enzyme
EDTA
restoration of activity by addition of divalent cations: Cu2+, Ni2+, Co2+ or Mg2+
EDTA
2 mM, 5% residual activity
EDTA
-
3 mM, complete inhibition
EDTA
-
complete inhibition at 1 mM
EDTA
-
decrease of the enzyme activity in presence of 0.5 mM EDTA
EDTA
-
inhibition of cytosolic and membrane-bound enzyme
EDTA
-
inhibition of membrane-bound enzyme
Fe2+
-
oxidative inactivation in a Fe2+/GSH system may be related to the selective association of Fe2+ and thiols to the enzyme molecule
Fe2+
further inhibition of the residual activity in EDTA-treated enzyme
Fe2+
-
the enzyme is susceptible to Fe2+-ion catalyzed oxidative modification. Among the examined chelators it is only deferoxamine and Na-citrate that exert a fully protective and reactivating ability, among the antioxidants it is only GSH, among the metal cations it is only Mn2+
Fe2+
-
plasma membrane enzyme
Fe3+
-
enzyme N-I and N-II
Fe3+
3.3% residual activity at 5 mM
fluoxetine
-
chronic treatment with 10mg/kg fluoxetine causes a 42% inhibition of AMP hydrolysis
GTP
-
HgCl2
-
63% inhibition at 1 mM, decreases AMP hydrolysis in a non-competitive manner
HgCl2
1 mM, about 20% inhibition
ITP
-
Mg2+
-
-
Mg2+
80.9% residual activity at 5 mM
Mn2+
-
plasma membrane enzyme
Mn2+
-
0.1 mM activate, 4 mM inhibit, plasma membrane enzyme
Mn2+
-
strong, enzyme from glioblastoma cells
Mn2+
-
inhibition of cytosolic and membrane-bound enzyme
Ni2+
-
plasma membrane enzyme
Ni2+
-
plasma membrane enzyme
Ni2+
-
strong, enzyme from glioblastoma cells
nortriptyline
-
chronic treatment with 10mg/kg nortriptyline causes a 30% inhibition of AMP hydrolysis
Pb2+
-
-
Pb2+
-
plasma membrane enzyme
phosphate
-
inhibition of cytosolic, 5'-IMP-specific enzyme
quercetin
-
-
sulfamic acid
-
tenascin C
-
strong inhibition
-
tenascin C
-
75.8% inhibition at 0.025 mg/ml
-
TTP
-
UTP
-
vanillic acid
-
vanillic acid
-
competitive inhibitor, complete inhibition at 0.6 mM
Zn2+
-
plasma membrane enzyme
Zn2+
-
3.0 mM, 75% inhibition of recombinant enzyme, 20% inhibition of native enzyme
Zn2+
2.1% residual activity at 5 mM
Zn2+
-
strong, enzyme from glioblastoma cells
Zn2+
-
inhibition of cytosolic and membrane-bound enzyme
Zn2+
about 30% enzymatic activity is achieved at 0.05 mM, and the activity drops further to about 15% for 0.5 mM or higher concentrations of Zn2+
Zn2+
5 mM, inhibition of both hydrolysis of AMP and ADP
additional information
-
no inhibition by sodium orthovanadate
-
additional information
-
not inhibitory: EDTA
-
additional information
-
ethanol causes no changes on 5'-nucleotidase activity in vivo
-
additional information
-
methanol does not cause any significant alteration on ecto-5'-nucleotidase activity
-
additional information
-
not inhibited by lead acetate
-
additional information
-
not inhibited by haloperidol, sulpiride and olanzapine
-
additional information
no substrate inhibition by p-nitrophenyl phosphate
-
additional information
-
no substrate inhibition by p-nitrophenyl phosphate
-
additional information
-
no product inhibition by 6-thioguanosine
-
additional information
-
not inhibited by levamisole
-
additional information
-
not inhibited by beta-glycerophosphate
-
additional information
-
not inhibitory: mycophenolic acid
-
additional information
-
in vitro and short-term in vivo treatment with fluoxetine and nortriptyline causes no change in activity
-
additional information
-
AMP hydrolysis by ecto-5'-nucleotidase is not affected by levamisole and tetramisole
-
additional information
-
not inhibited by levamisole
-
additional information
-
endogenous inhibitor from rat tissues
-
additional information
-
insensitive to levamisole, tetramisole and adenosine 5'-[alpha-beta-methylene]diphosphate
-
additional information
-
not inhibited by tetramisole
-
additional information
-
no inhibition of the enzyme by phosphatase inhibitors. i.e. sodium orthovanadate and sodium fluoride
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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0.02
(Polyphosphate)n
+/- 0.003
0.0065
1,N6-ethenoadenosine 5'-phosphate
-
-
0.007
3-beta-ribofuranosyladenine 5'-monophosphate
-
-
0.224 - 21.78
4-nitrophenyl phosphate
0.41 - 0.83
5'-inosinic acid
1.27
5-phosphoribosyl-1-diphosphate
at pH 8.9 and 25°C
0.0053
6-chloropurine ribonucleoside 5'-monophosphate
-
-
10
9-beta-D-arabinosyl-2-fluoroadenine monophosphate
-
pH 8.3, 25°C
0.002
adenosine 5'-O-thiophosphate
-
-
0.28
bis-p-nitrophenyl phosphate
-
-
24
D-ribose 5-phosphate
at pH 8.9 and 25°C
6.34
IMP
-
in 40 mM sodium citrate buffer, 0.1 mM dithiothreitol, 110 mM NaCl, 20 mM MgCl2, and 20 mM CaCl2, at 50°C
0.135
MgAMP
-
pH 7.2, 37°C
0.87
p-Nitrophenyl phenylphosphonate
-
-
4.741 - 5.355
p-nitrophenyl phosphate
additional information
additional information
-
0.224
4-nitrophenyl phosphate
pH 7.0, 22°C
1.64
4-nitrophenyl phosphate
at pH 8.9 and 25°C
2.09
4-nitrophenyl phosphate
+/- 0.13
2.49
4-nitrophenyl phosphate
+/- 0.4
5.713
4-nitrophenyl phosphate
-
wild-type, pH 7.0, 22°C
21.78
4-nitrophenyl phosphate
-
N-terminal protein domain, pH 7.0, 22°C
0.0003
5'-AMP
-
-
0.00063
5'-AMP
-
pH 8.5, 37°C
0.001
5'-AMP
-
pH 8.0, 37°C
0.0018
5'-AMP
-
pH 8.5, 37°C, strain KL4
0.0022
5'-AMP
-
pH 8.5, 37°C, strain incognitus
0.0026
5'-AMP
-
pH 8.5, 37°C, strain KL8, strain BRO and strain M106
0.0038
5'-AMP
37°C, pH not specified in the publication
0.0041
5'-AMP
-
pH 8.5, 37°C, strain GIM
0.0042
5'-AMP
-
pH 8.5, 37°C, strain No.29
0.0104
5'-AMP
-
pH 8.5, 37°C, strain No.5
0.011
5'-AMP
mutant P90C/L424C, pH 7.5, 25°C
0.0119
5'-AMP
-
pH 7.4, 37°C, enzyme from dystrophic muscle
0.0121
5'-AMP
-
pH 7.4, 37°C, enzyme from normal muscle
0.0129
5'-AMP
-
wild-type, pH 7.0, 22°C
0.0153
5'-AMP
-
enzyme purified from plasma membrane
0.02
5'-AMP
-
enzyme from glioblastoma cells
0.021
5'-AMP
-
membrane-bound enzyme
0.0307
5'-AMP
-
cytosolic enzyme
0.035
5'-AMP
-
fraction HL, sarcolemma
0.06
5'-AMP
-
isoenzyme 5'-NT-2 and 5'-NT-4
0.074
5'-AMP
wild-type, pH 7.5, 25°C
0.083
5'-AMP
-
synaptic membrane-bound
0.0987
5'-AMP
-
plasma membrane-bound enzyme
0.11
5'-AMP
-
isoenzyme 5'-NT-1 and 5'-NT-3
0.117
5'-AMP
-
pH 7.4, 37°C
0.12
5'-AMP
-
enzyme N-II
0.13
5'-AMP
-
fraction S, sarcolemma
0.1683
5'-AMP
wild type enzyme, at pH 7.4 and 37°C
0.27
5'-AMP
-
brain cytosol
0.275
5'-AMP
mutant enzyme F414A, at pH 7.4 and 37°C
0.29
5'-AMP
-
37°C, pH 7.0, presence of 1 mM ADP
0.49
5'-AMP
-
pH 7.2, 36°C
0.58
5'-AMP
-
enzyme from plasma membrane
0.8
5'-AMP
Km above 0.8 mM, mutant enzyme R392A, at pH 7.4 and 37°C
1.02
5'-AMP
-
enzyme from Golgi apparatus
1.2
5'-AMP
-
cytosolic enzyme c-N-I
1.46
5'-AMP
-
partially purified enzyme isolated from heart
1.85
5'-AMP
-
37°C, pH 7.0
1.87
5'-AMP
pH 7.0, 37°C, in presence of 1 mM ADP
1.9
5'-AMP
-
recombinant enzyme from heart
2.85
5'-AMP
-
cytosolic enzyme
2.96
5'-AMP
pH 7.0, 37°C, without ADP
4.8
5'-AMP
-
pH 6.5, 37°C
6.3
5'-AMP
-
presence of 10 mM ATP
7.1
5'-AMP
-
37°C, pH 7.5
7.3
5'-AMP
-
37°C, pH 7.5
13.6
5'-AMP
-
37°C, pH 7.5
0.002
5'-CMP
-
-
0.0564
5'-CMP
37°C, pH not specified in the publication
0.0631
5'-CMP
-
enzyme purified from plasma membrane
0.07
5'-CMP
-
enzyme from glioblastoma cells
0.11
5'-CMP
-
cytosolic enzyme c-N-I
0.111
5'-CMP
-
plasma membrane-bound enzyme
0.14
5'-CMP
-
synaptic membrane-bound
0.19
5'-CMP
-
brain cytosol
0.207
5'-CMP
-
membrane-bound enzyme
0.4
5'-CMP
-
enzyme N-I, enzyme N-II
2.3
5'-CMP
-
5'-nucleotidase Sdt1, pH not specified in the publication, temperature not specified in the publication
4.05
5'-CMP
-
cytosolic enzyme
4.5
5'-CMP
-
presence of 10 mM ATP
0.012
5'-dAMP
+/- 0.001
0.114
5'-dAMP
37°C, pH not specified in the publication
0.13
5'-dAMP
-
cytosolic enzyme c-N-I
2 - 3
5'-dAMP
-
37°C, pH 7.0
5.2
5'-dAMP
-
37°C, pH 7.0, presence of 1 mM ADP
0.023
5'-dCMP
-
-
0.04
5'-dCMP
-
37°C, pH 7.0, presence of 1 mM ADP
0.061
5'-dCMP
-
cytosolic enzyme c-N-I
0.75
5'-dCMP
-
37°C, pH 7.0
0.044
5'-dGMP
-
-
142
5'-dGMP
sigmoidal kinetic profile, pH 8.0, temperature not specified in the publication
0.017
5'-dIMP
+/- 0.001
0.008
5'-dTMP
+/- 0.0003
0.017
5'-dUMP
-
-
0.006386
5'-GMP
-
at pH 7.5 and 37°C
0.0065
5'-GMP
37°C, pH not specified in the publication
0.038
5'-GMP
-
enzyme from glioblastoma cells
0.0416
5'-GMP
-
enzyme purified from plasma membrane
0.048
5'-GMP
-
synaptic membrane-bound
0.07
5'-GMP
-
isoenzyme 5'-NT-4
0.0712
5'-GMP
-
plasma membrane-bound enzyme
0.09
5'-GMP
-
brain cytosol
0.09
5'-GMP
-
isoenzyme 5'-NT-2
0.14
5'-GMP
-
presence of 10 mM ATP
0.16
5'-GMP
-
isoenzyme 5'-NT-3
0.178
5'-GMP
-
membrane-bound enzyme
0.215
5'-GMP
-
cytosolic enzyme
0.22
5'-GMP
-
isoenzyme 5'-NT-1
0.32
5'-GMP
-
cytosolic enzyme c-N-I
0.4
5'-GMP
-
enzyme N-I, enzyme N-II
4.76
5'-GMP
-
cytosolic enzyme
6.65
5'-GMP
at pH 8.9 and 25°C
7.2
5'-GMP
sigmoidal kinetic profile, pH 8.0, temperature not specified in the publication
0.0071
5'-IMP
37°C, pH not specified in the publication
0.014
5'-IMP
-
enzyme from glioblastoma cells
0.04
5'-IMP
pH not specified in the publication, temperature not specified in the publication, recombinant mutant K311A
0.05
5'-IMP
pH not specified in the publication, temperature not specified in the publication, recombinant mutant G319D
0.0519
5'-IMP
-
enzyme purified from plasma membrane
0.056
5'-IMP
-
synaptic membrane-bound
0.06
5'-IMP
-
isoenzyme 5'-NT-3
0.06
5'-IMP
pH not specified in the publication, temperature not specified in the publication, recombinant mutant Y115A
0.061
5'-IMP
-
membrane-bound enzyme
0.0622
5'-IMP
-
cytosolic enzyme
0.07
5'-IMP
-
isoenzyme 5'-NT-2 and 5'-NT-4
0.09
5'-IMP
-
presence of 3 mM ATP
0.1
5'-IMP
-
mutant S251A
0.1
5'-IMP
pH not specified in the publication, temperature not specified in the publication, recombinant wild-type enzyme and mutant D396A
0.11
5'-IMP
-
presence of 10 mM ATP
0.116
5'-IMP
-
plasma membrane-bound enzyme
0.13
5'-IMP
-
presence of 3 mM ATP plus 4 mM phosphate
0.15
5'-IMP
-
isoenzyme 5'-NT-1
0.2
5'-IMP
-
brain cytosol
0.2
5'-IMP
-
mutant D356E
0.2
5'-IMP
-
mutant D356N
0.2
5'-IMP
-
mutant T249S
0.3
5'-IMP
-
mutant S251T
0.4
5'-IMP
-
mutant T249v
0.41
5'-IMP
-
pH 6.5, 37°C
0.54
5'-IMP
-
presence of 4 mM phosphate
1
5'-IMP
-
cytosolic enzyme c-N-I
1.67
5'-IMP
-
37°C, pH 7.0, presence of 1 mM ADP
2.47
5'-IMP
pH 7.0, 37°C, in presence of 1 mM ADP
2.85
5'-IMP
-
cytosolic enzyme
10.8
5'-IMP
-
37°C, pH 7.0
12.11
5'-IMP
pH 7.0, 37°C, without ADP
18
5'-IMP
biphasic kinetic profile, pH 8.0, temperature not specified in the publication
0.41
5'-inosinic acid
-
37°C, pH 7.5
0.58
5'-inosinic acid
-
37°C, pH 7.5
0.83
5'-inosinic acid
-
37°C, pH 7.5
0.008
5'-TMP
37°C, pH not specified in the publication
0.02
5'-TMP
-
cytosolic enzyme c-N-I
0.048
5'-TMP
-
synaptic membrane-bound
0.074
5'-TMP
-
brain cytosol
0.014
5'-UMP
-
-
0.031
5'-UMP
-
enzyme from glioblastoma cells
0.0316
5'-UMP
37°C, pH not specified in the publication
0.0745
5'-UMP
-
enzyme purified from plasma membrane
0.09
5'-UMP
-
synaptic membrane-bound
0.0918
5'-UMP
-
plasma membrane-bound enzyme
0.131
5'-UMP
-
cytosolic enzyme
0.175
5'-UMP
-
membrane-bound enzyme
0.18
5'-UMP
-
brain cytosol
1.1
5'-UMP
wild-type, pH 7.5, 37°C
1.1
5'-UMP
mutant S117G, pH 7.5, 37°C
1.1
5'-UMP
mutant T66G, pH 7.5, 37°C
1.2
5'-UMP
-
5'-nucleotidase Sdt1, pH not specified in the publication, temperature not specified in the publication
2.1
5'-UMP
mutant N69G, pH 7.5, 37°C
2.3
5'-UMP
mutant Y114A, pH 7.5, 37°C
2.5
5'-UMP
-
presence of 10 mM ATP
2.56
5'-UMP
-
cytosolic enzyme
3.2
5'-UMP
mutant H68A, pH 7.5, 37°C
4.8
5'-UMP
mutant E96S, pH 7.5, 37°C
29
5'-UMP
mutant W11A, pH 7.5, 37°C
0.065
5'-XMP
-
-
0.99
5'-XMP
-
enzyme N-II
1.53
5'-XMP
at pH 8.9 and 25°C
0.00009
ADP
-
-
0.0547
ADP
-
wild-type, pH 7.0, 22°C
10.15
ADP
-
N-terminal protein domain, pH 7.0, 22°C
0.0091
AMP
-
at pH 8.5
0.0387
AMP
-
using 2.0 mM MgCl2, in 150 mM sucrose and 50 mM glycine, at pH 9.5 and 37°C
0.0597
AMP
-
enzyme from soluble fraction
0.1348
AMP
-
enzyme from microsomal fraction
0.466
AMP
-
in 50 mM Tris buffer (pH 7.2) and 3.0 mM MgCl2, at 37°C
1.49
AMP
-
in 40 mM sodium citrate buffer, 0.1 mM dithiothreitol, 110 mM NaCl, 20 mM MgCl2, and 20 mM CaCl2, at 50°C
0.0001
ATP
-
-
0.0209
ATP
-
wild-type, pH 7.0, 22°C
6.807
ATP
-
N-terminal protein domain, pH 7.0, 22°C
0.012
dCMP
pH 7.0, 37°C, in presence of 1 mM ADP
0.03
dCMP
-
isoenzyme 5'-NT-3
0.04
dCMP
-
isoenzyme 5'-NT-2
0.05
dCMP
-
isoenzyme 5'-NT-1
0.06
dCMP
-
isoenzyme 5'-NT-4
0.193
dCMP
pH 7.0, 37°C, without ADP
0.25
Inosine
-
mutant T249v
0.5
Inosine
-
mutant K292M
0.8
Inosine
-
mutant M53I
0.9
Inosine
-
mutant D351E
0.9
Inosine
-
mutant T249S
1.1
Inosine
-
mutant M53N
1.5
Inosine
-
mutant S251T
1.8
Inosine
presence of 0.4 mM 2,3-bisphosphoglycerate, Tris-buffer, pH 7.4
2.17
Inosine
presence of 2 mM ATP, Tris-buffer, pH 7.4
10.2
Inosine
-
mutant D356N
4.741
p-nitrophenyl phosphate
wild-type-type, pH 7.5, 25°C
5.355
p-nitrophenyl phosphate
mutant P90C/L424C, pH 7.5, 25°C
0.024
ribavirin
presence of 2 mM ATP, phosphate-buffered saline, pH 7.4
0.047
ribavirin
presence of 0.4 mM 2,3-bisphosphoglycerate, Tris-buffer, pH 7.4
0.088
ribavirin
presence of 0.4 mM 2,3-bisphosphoglycerate, phosphate-buffered saline, pH 7.4
0.095
ribavirin
presence of 2 mM ATP, Tris-buffer, pH 7.4
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
Km-value for viramidine is above 0.5 mM
-
additional information
additional information
-
complex allosteric regulation of cytosolic 5'-nucleotidase II
-
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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0.0013
(2E)-5-(5-methylfuran-2-yl)-N-(2-methylphenyl)-3-oxo-2-(2-oxo-1,2-dihydro-3H-indol-3-ylidene)-2,3-dihydro-5H-[1,3]thiazolo[3,2-a]pyrimidine-6-carboxamide
at pH 7.0 and 37°C
1.14
1-(6-deoxy-6-phosphono-beta-D-allofuranosyl)-5-(2-(E)-phenylvinyl)-cytosine
pH 6.5, 37°C
2.01
1-(6-deoxy-6-phosphono-beta-D-allofuranosyl)-5-phenyl-cytosine
pH 6.5, 37°C
1.74
1-(6-deoxy-6-phosphono-beta-D-allofuranosyl)-cytosine
pH 6.5, 37°C
0.00293
1-amino-4-(1-anthracenylamino)-9,10-dioxo-9,10 dihydroanthracene-2-sulfonate
-
-
0.00053
1-amino-4-(1-naphthylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
0.00015
1-amino-4-(2-anthracenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
0.00026
1-amino-4-(2-carboxy-4-fluorophenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
0.00653
1-amino-4-(2-carboxyphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
0.00184
1-amino-4-(2-hydroxyphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
0.00274
1-amino-4-(2-methyl-1-naphthylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
0.00147
1-amino-4-(2-naphthylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
0.036
1-amino-4-(3-amino-2,4,6-trimethylphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
0.0252
1-amino-4-(3-amino-4-sulfophenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
0.0123
1-amino-4-(3-methylphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
0.01
1-amino-4-(4-amino-3-sulfophenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
0.000297
1-amino-4-(4-aminophenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
0.0113
1-amino-4-(4-bromophenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
0.00481
1-amino-4-(4-chlorophenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
0.00567
1-amino-4-(4-fluorophenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
0.00062
1-amino-4-(4-hydroxyphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
0.36
1-amino-4-(4-methylphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
0.00151
1-amino-4-(4-phenoxyphenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
0.00343
1-amino-4-(4-sulfophenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
0.0117
1-amino-4-(5-sulfo-1-naphthylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
0.00343
1-amino-4-(6-carboxy-2-naphthylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
0.0153
1-amino-4-(6-sulfo-1-naphthylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
0.00605
1-amino-4-(8-sulfo-1-naphthylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
0.00166
1-amino-4-(cyclohexylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
-
-
0.0172
2,3,5-tri-O-benzyl-1-O-(4-nitrobenzoyl)-D-arabinofuranose
at pH 7.0 and 37°C
0.0012
2-((4'-cyano-4,4''-dimethoxy-[1,1':3',1''-terphenyl]-5'-yl)thio)-N-(3-cyano-4,5,6,7-tetrahydrobenzo[b]thiophen-2-yl)acetamide
at pH 7.0 and 37°C
0.00122
2-(3,4-dihydroisoquinolin-2(1H)-yl)-N-[4-[1-(4-methylbenzoyl)-2,3-dihydro-1H-indol-5-yl]-5-sulfanyl-1,3-thiazol-2-yl]acetamide
at pH 7.0 and 37°C
13
2-mercaptoethanol
-
pH 7.4, 38°C
0.00053
3-[4,5-di([1,1'-biphenyl]-4-yl)-1H-imidazol-2-yl]-1H-indole
at pH 7.0 and 37°C
0.00046
4-(4-[1-[(3-chlorophenyl)methyl]-4-hydroxy-1H-[1,2,3]triazolo[4,5-c]pyridin-6-yl]piperidine-1-sulfonyl)-N-cycloheptylbenzene-1-sulfonamide
at pH 7.0 and 37°C
0.0061
4-(6-[[2-(4-aminophenyl)-1H-benzimidazol-5-yl]methyl]-1H-benzimidazol-2-yl)aniline
at pH 7.0 and 37°C
0.0027
6-(4-ethoxyphenyl)-3-(4-methylphenyl)-N-(2-phenylethyl)-1H-pyrazolo[3,4-b]pyridine-4-carboxamide
at pH 7.0 and 37°C
0.00051 - 0.00087
alpha,beta-methylene-ADP
0.00036 - 0.00045
Ap[CH2]p
0.000035
Con A
-
pH 7.4, 37°C, enzyme from dystrophic muscle
-
0.000022
concanavalin A
-
pH 7.4, 37°C, enzyme from normal muscle
-
0.041
Cys-Gly
-
pH 7.4, 38°C
2.4
cysteamine
-
pH 7.4, 38°C
0.09
dithiothreitol
-
pH 7.4, 38°C
0.00068
ethyl 4-(2-((4'-cyano-4''-methoxy-4-methyl-[1,1':3',1''-terphenyl]-5'-yl)thio)acetamido)benzoate
at pH 7.0 and 37°C
3.7
gamma-glutamylcysteine
-
pH 7.4, 38°C
0.32
L-cysteine
-
pH 7.4, 38°C
0.009
L-cysteine ethyl ester
-
pH 7.4, 38°C
0.0011
myricetin
pH and temperature not specified in the publication
0.00939
N-(4-methylcyclohexyl)-1-(2-phenyl-1H-benzimidazole-5-sulfonyl)piperidine-3-carboxamide
at pH 7.0 and 37°C
3.2
N-acetylcysteine
-
pH 7.4, 38°C
0.0049
N-[4-[5-([2-[2-(4-methoxybenzoyl)hydrazinyl]-2-oxoethyl]sulfanyl)-4-(4-methoxyphenyl)-4H-1,2,4-triazol-3-yl]phenyl]-4-methylbenzene-1-sulfonamide
at pH 7.0 and 37°C
0.0014
quercetin
pH and temperature not specified in the publication
0.00026
sodium 1-amino-4-(2-carboxy-4-fluoroanilino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
pH and temperature not specified in the publication
0.00015
sodium 1-amino-4-[(anthracen-2-yl)amino]-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate
pH and temperature not specified in the publication
0.0152
sodium 1-amino-9,10-dioxo-4-(phenylamino)-9,10-dihydroanthracene-2-sulfonate
-
-
0.00307
tetrasodium 4-[[4-([4-[(4-amino-9,10-dioxo-3-sulfonato-9,10-dihydroanthracen-1-yl)amino]-2-sulfonatophenyl]amino)-6-chloro-1,3,5-triazin-2-yl]amino]benzene-1,2-disulfonate
-
-
6
5'-AMP
mutant P90C/L424C, pH 7.5, 25°C
23
5'-AMP
wild-type, pH 7.5, 25°C
0.00091
ADP
-
in 10 mM HEPES (pH 7.4), 2 mM MgCl2, and 1 mM CaCl2
0.00091
ADP
pH and temperature not specified in the publication
0.0075
ADP
-
pH 7.4, 37°C, enzyme from normal muscle
0.0098
ADP
-
pH 7.4, 37°C, enzyme from dystrophic muscle
0.00051
alpha,beta-methylene-ADP
-
in 10 mM HEPES (pH 7.4), 2 mM MgCl2, and 1 mM CaCl2
0.00087
alpha,beta-methylene-ADP
-
in 10 mM HEPES (pH 7.4), 2 mM MgCl2, and 1 mM CaCl2
0.00036
Ap[CH2]p
-
pH 7.4, 37°C, enzyme from normal muscle
0.00045
Ap[CH2]p
-
pH 7.4, 37°C, enzyme from dystrophic muscle
0.0089
ATP
-
in 10 mM HEPES (pH 7.4), 2 mM MgCl2, and 1 mM CaCl2
0.0138
ATP
-
pH 7.4, 37°C, enzyme from dystrophic muscle
0.0155
ATP
-
pH 7.4, 37°C, enzyme from normal muscle
24.1
ATP
with 3'-AMP as substrate
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.004 - 0.027
1-amino-4-bromo-9,10-dioxo-9,10-dihydroanthracene-2-sulfonic acid
0.00729 - 0.0137
1-pentyl-1H-benzofuro[3,2-b]pyrrolo[3,2-e]pyridine-3-carbonitrile
0.00543 - 0.00921
1-tert-butyl-1H-benzofuro[3,2-b]pyrrolo[3,2-e]pyridine-3-carbonitrile
0.1
1-tert-butyl-5-chloro-6-(2-hydroxy-5-methylphenyl)-1H-pyrrolo[2,3-b]pyridine-3-carbonitrile
0.1
1-tert-butyl-5-chloro-6-(2-hydroxyphenyl)-1H-pyrrolo[2,3-b]pyridine-3-carbonitrile
0.1
2-(3-methyl-1,5-diphenyl-1H-pyrazolo[3,4-b]pyridin-6-yl)phenol
0.00326 - 0.0235
2-(5-chloro-1,3-dimethyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-4-methylphenol
0.00203 - 0.00263
2-(5-chloro-1,3-dimethyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-5-methoxyphenol
0.00032 - 0.1
2-(5-chloro-3-methyl-1-p-tolyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-4-methylphenol
0.00346 - 0.0111
2-(5-chloro-3-methyl-1-p-tolyl-1H-pyrazolo[3,4-b]pyridin-6-yl)phenol
0.00573 - 0.1
2-(5-chloro-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-4-methylphenol
0.00067 - 0.0105
2-(5-chloro-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-5-methoxyphenol
0.1
2-(5-chloro-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridin-6-yl)phenol
0.00077
2-chloro-N-(pyridin-4-yl)benzamide
Homo sapiens
pH and temperature not specified in the publication
0.0112
2-methyl-N-(pyridin-4-yl)benzamide
Homo sapiens
pH and temperature not specified in the publication
0.00038
2-nitro-N-(pyridin-4-yl)benzamide
Homo sapiens
pH and temperature not specified in the publication
0.00041
2-phenyl-N-(pyridin-4-yl)acetamide
Homo sapiens
pH and temperature not specified in the publication
0.00307
3,4,5-trimethoxy-N-(pyridin-4-yl)benzamide
Homo sapiens
pH and temperature not specified in the publication
0.00554
3,5-dinitro-N-(pyridine-4-yl)benzamide
Homo sapiens
pH and temperature not specified in the publication
0.00463 - 0.1
3,8-dimethyl-1-p-tolyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine
0.00566 - 0.1
3,8-dimethyl-1-phenyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine
0.0102
3-(2-(3,4,5-trimethoxybenzylideneamino)thiazol-4-yl)-2Hchromen-2-one
Homo sapiens
-
at pH 7.4 and 37°C
0.00223
3-(2-(3-chlorobenzylideneamino)thiazol-4-yl)-2H-chromen-2-one
Homo sapiens
-
at pH 7.4 and 37°C
0.00041
3-(2-(3-methoxy-4-phenoxybenzylideneamino)thiazol-4-yl)-2H-chromen-2-one
Homo sapiens
-
at pH 7.4 and 37°C
0.00025
3-(2-(3-nitrobenzylideneamino)thiazol-4-yl)-2H-chromen-2-one
Homo sapiens
-
at pH 7.4 and 37°C
0.000438
3-(2-(4-bromobenzylideneamino)thiazol-4-yl)-2H-chromen-2-one
Homo sapiens
-
at pH 7.4 and 37°C
0.00028
3-(2-(4-chlorobenzylideneamino)thiazol-4-yl)-2H-chromen-2-one
Homo sapiens
-
at pH 7.4 and 37°C
0.00515
3-(2-(4-fluorobenzylideneamino)thiazol-4-yl)-2H-chromen-2-one
Homo sapiens
-
at pH 7.4 and 37°C
0.00092
3-(2-(furan-2-ylmethyleneamino)thiazol-4-yl)-2H-chromen-2-one
Homo sapiens
-
at pH 7.4 and 37°C
0.00411
3-chloro-N-(pyridin-4-yl)benzamide
Homo sapiens
pH and temperature not specified in the publication
0.00732 - 0.1
3-methyl-1-p-tolyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine
0.00645 - 0.0111
3-methyl-1-phenyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine
0.00025
3-methyl-N-(pyridin-4-yl)benzamide
Homo sapiens
pH and temperature not specified in the publication
0.00109
3-nitro-N-(pyridin-4-yl)benzamide
Homo sapiens
pH and temperature not specified in the publication
0.0061 - 0.0443
4-(3-methyl-5-oxo-4,5-dihydro-1H-pyrazol-1-yl)benzenesulfonic acid
0.00029
4-chloro-N-(pyridin-4-yl)benzamide
Homo sapiens
pH and temperature not specified in the publication
0.00171
4-methyl-N-(pyridin-4-yl)benzamide
Homo sapiens
pH and temperature not specified in the publication
0.00042
4-nitro-N-(pyridin-4-yl)benzamide
Homo sapiens
pH and temperature not specified in the publication
0.0878
5'-deoxy-7-methyl-5'-(4-phosphono-1H-pyrazol-1-yl)guanosine
Homo sapiens
-
at pH 7.5 and 30°C
0.1
5-chloro-3-methyl-1-phenyl-6-(2-propoxyphenyl)-1H-pyrazolo[3,4-b]pyridine
0.1
5-chloro-6-(2-(heptyloxy)phenyl)-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridine
0.1
5-chloro-6-(2-hydroxy-5-methylphenyl)-2-phenyl-2H-pyrazolo[3,4-b]pyridin-3-ol
0.0103 - 0.1
5-chloro-6-(2-hydroxyphenyl)-2-phenyl-2H-pyrazolo[3,4-b]pyridin-3-ol
0.0184 - 0.1
5-chloro-6-(2-methoxyphenyl)-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridine
0.02
5-ethynyldideoxyuridine
Homo sapiens
-
-
0.00132 - 0.0104
6-amino-4-hydroxynaphthalene-2-sulfonic acid
0.00352 - 0.0171
6-chloro-7-(2-hydroxy-5-methylphenyl)pyrido[2,3-d]pyrimidine-2,4(1H,3H)-dione
0.00129 - 0.00214
6-chloro-7-(2-hydroxyphenyl)-1-methylpyrido[2,3-d]pyrimidine-2,4(1H,3H)-dione
0.00521 - 0.1
6-chloro-7-(2-hydroxyphenyl)pyrido[2,3-d]pyrimidine-2,4(1H,3H)-dione
0.0124 - 0.1
7-methoxy-3-methyl-1-phenyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine
0.0024
concanavalin A
Naja naja
-
in 50 mM Tris-HCl buffer, pH 7.4, 10 mM MgCl2, 50 mM NaCl, 10 mM KCl, at 37°C
-
0.00792
N-(pyridin-4-yl)furan-2-carboxamide
Homo sapiens
pH and temperature not specified in the publication
0.0453
quercetin
Homo sapiens
-
pH 7.4, 37°C
0.0421 - 0.0773
sulfamic acid
0.0421
Sulfanilic acid
Homo sapiens
pH and temperature not specified in the publication
0.18
vanillic acid
Naja naja
-
in 50 mM Tris-HCl buffer, pH 7.4, 10 mM MgCl2, 50 mM NaCl, 10 mM KCl, at 37°C
0.004
1-amino-4-bromo-9,10-dioxo-9,10-dihydroanthracene-2-sulfonic acid
Homo sapiens
-
pH 7.4, 37°C
0.027
1-amino-4-bromo-9,10-dioxo-9,10-dihydroanthracene-2-sulfonic acid
Rattus norvegicus
-
pH 7.4, 37°C
0.00729
1-pentyl-1H-benzofuro[3,2-b]pyrrolo[3,2-e]pyridine-3-carbonitrile
Rattus norvegicus
at pH 7.5 and 37°C
0.0137
1-pentyl-1H-benzofuro[3,2-b]pyrrolo[3,2-e]pyridine-3-carbonitrile
Homo sapiens
at pH 7.5 and 37°C
0.00543
1-tert-butyl-1H-benzofuro[3,2-b]pyrrolo[3,2-e]pyridine-3-carbonitrile
Rattus norvegicus
at pH 7.5 and 37°C
0.00921
1-tert-butyl-1H-benzofuro[3,2-b]pyrrolo[3,2-e]pyridine-3-carbonitrile
Homo sapiens
at pH 7.5 and 37°C
0.1
1-tert-butyl-5-chloro-6-(2-hydroxy-5-methylphenyl)-1H-pyrrolo[2,3-b]pyridine-3-carbonitrile
Homo sapiens
IC50 above 0.1 mM, at pH 7.5 and 37°C
0.1
1-tert-butyl-5-chloro-6-(2-hydroxy-5-methylphenyl)-1H-pyrrolo[2,3-b]pyridine-3-carbonitrile
Rattus norvegicus
IC50 above 0.1 mM, at pH 7.5 and 37°C
0.1
1-tert-butyl-5-chloro-6-(2-hydroxyphenyl)-1H-pyrrolo[2,3-b]pyridine-3-carbonitrile
Homo sapiens
IC50 above 0.1 mM, at pH 7.5 and 37°C
0.1
1-tert-butyl-5-chloro-6-(2-hydroxyphenyl)-1H-pyrrolo[2,3-b]pyridine-3-carbonitrile
Rattus norvegicus
IC50 above 0.1 mM, at pH 7.5 and 37°C
0.1
2-(3-methyl-1,5-diphenyl-1H-pyrazolo[3,4-b]pyridin-6-yl)phenol
Homo sapiens
IC50 above 0.1 mM, at pH 7.5 and 37°C
0.1
2-(3-methyl-1,5-diphenyl-1H-pyrazolo[3,4-b]pyridin-6-yl)phenol
Rattus norvegicus
IC50 above 0.1 mM, at pH 7.5 and 37°C
0.00326
2-(5-chloro-1,3-dimethyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-4-methylphenol
Homo sapiens
at pH 7.5 and 37°C
0.0235
2-(5-chloro-1,3-dimethyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-4-methylphenol
Rattus norvegicus
at pH 7.5 and 37°C
0.00203
2-(5-chloro-1,3-dimethyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-5-methoxyphenol
Rattus norvegicus
at pH 7.5 and 37°C
0.00263
2-(5-chloro-1,3-dimethyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-5-methoxyphenol
Homo sapiens
at pH 7.5 and 37°C
0.00032
2-(5-chloro-3-methyl-1-p-tolyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-4-methylphenol
Homo sapiens
at pH 7.5 and 37°C
0.1
2-(5-chloro-3-methyl-1-p-tolyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-4-methylphenol
Rattus norvegicus
IC50 above 0.1 mM, at pH 7.5 and 37°C
0.00346
2-(5-chloro-3-methyl-1-p-tolyl-1H-pyrazolo[3,4-b]pyridin-6-yl)phenol
Homo sapiens
at pH 7.5 and 37°C
0.0111
2-(5-chloro-3-methyl-1-p-tolyl-1H-pyrazolo[3,4-b]pyridin-6-yl)phenol
Rattus norvegicus
at pH 7.5 and 37°C
0.00573
2-(5-chloro-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-4-methylphenol
Homo sapiens
at pH 7.5 and 37°C
0.1
2-(5-chloro-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-4-methylphenol
Rattus norvegicus
IC50 above 0.1 mM, at pH 7.5 and 37°C
0.00067
2-(5-chloro-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-5-methoxyphenol
Rattus norvegicus
at pH 7.5 and 37°C
0.0105
2-(5-chloro-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridin-6-yl)-5-methoxyphenol
Homo sapiens
at pH 7.5 and 37°C
0.1
2-(5-chloro-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridin-6-yl)phenol
Homo sapiens
IC50 above 0.1 mM, at pH 7.5 and 37°C
0.1
2-(5-chloro-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridin-6-yl)phenol
Rattus norvegicus
IC50 above 0.1 mM, at pH 7.5 and 37°C
0.00463
3,8-dimethyl-1-p-tolyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine
Homo sapiens
at pH 7.5 and 37°C
0.1
3,8-dimethyl-1-p-tolyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine
Rattus norvegicus
IC50 above 0.1 mM, at pH 7.5 and 37°C
0.00566
3,8-dimethyl-1-phenyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine
Homo sapiens
at pH 7.5 and 37°C
0.1
3,8-dimethyl-1-phenyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine
Rattus norvegicus
IC50 above 0.1 mM, at pH 7.5 and 37°C
0.00732
3-methyl-1-p-tolyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine
Homo sapiens
at pH 7.5 and 37°C
0.1
3-methyl-1-p-tolyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine
Rattus norvegicus
IC50 above 0.1 mM, at pH 7.5 and 37°C
0.00645
3-methyl-1-phenyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine
Rattus norvegicus
at pH 7.5 and 37°C
0.0111
3-methyl-1-phenyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine
Homo sapiens
at pH 7.5 and 37°C
0.0061
4-(3-methyl-5-oxo-4,5-dihydro-1H-pyrazol-1-yl)benzenesulfonic acid
Homo sapiens
-
pH 7.4, 37°C
0.0443
4-(3-methyl-5-oxo-4,5-dihydro-1H-pyrazol-1-yl)benzenesulfonic acid
Rattus norvegicus
-
pH 7.4, 37°C
0.1
5-chloro-3-methyl-1-phenyl-6-(2-propoxyphenyl)-1H-pyrazolo[3,4-b]pyridine
Homo sapiens
IC50 above 0.1 mM, at pH 7.5 and 37°C
0.1
5-chloro-3-methyl-1-phenyl-6-(2-propoxyphenyl)-1H-pyrazolo[3,4-b]pyridine
Rattus norvegicus
IC50 above 0.1 mM, at pH 7.5 and 37°C
0.1
5-chloro-6-(2-(heptyloxy)phenyl)-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridine
Homo sapiens
IC50 above 0.1 mM, at pH 7.5 and 37°C
0.1
5-chloro-6-(2-(heptyloxy)phenyl)-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridine
Rattus norvegicus
IC50 above 0.1 mM, at pH 7.5 and 37°C
0.1
5-chloro-6-(2-hydroxy-5-methylphenyl)-2-phenyl-2H-pyrazolo[3,4-b]pyridin-3-ol
Homo sapiens
IC50 above 0.1 mM, at pH 7.5 and 37°C
0.1
5-chloro-6-(2-hydroxy-5-methylphenyl)-2-phenyl-2H-pyrazolo[3,4-b]pyridin-3-ol
Rattus norvegicus
IC50 above 0.1 mM, at pH 7.5 and 37°C
0.0103
5-chloro-6-(2-hydroxyphenyl)-2-phenyl-2H-pyrazolo[3,4-b]pyridin-3-ol
Homo sapiens
at pH 7.5 and 37°C
0.1
5-chloro-6-(2-hydroxyphenyl)-2-phenyl-2H-pyrazolo[3,4-b]pyridin-3-ol
Rattus norvegicus
IC50 above 0.1 mM, at pH 7.5 and 37°C
0.0184
5-chloro-6-(2-methoxyphenyl)-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridine
Homo sapiens
at pH 7.5 and 37°C
0.1
5-chloro-6-(2-methoxyphenyl)-3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridine
Rattus norvegicus
IC50 above 0.1 mM, at pH 7.5 and 37°C
0.00132
6-amino-4-hydroxynaphthalene-2-sulfonic acid
Homo sapiens
-
pH 7.4, 37°C
0.0104
6-amino-4-hydroxynaphthalene-2-sulfonic acid
Rattus norvegicus
-
pH 7.4, 37°C
0.00352
6-chloro-7-(2-hydroxy-5-methylphenyl)pyrido[2,3-d]pyrimidine-2,4(1H,3H)-dione
Rattus norvegicus
at pH 7.5 and 37°C
0.0171
6-chloro-7-(2-hydroxy-5-methylphenyl)pyrido[2,3-d]pyrimidine-2,4(1H,3H)-dione
Homo sapiens
at pH 7.5 and 37°C
0.00129
6-chloro-7-(2-hydroxyphenyl)-1-methylpyrido[2,3-d]pyrimidine-2,4(1H,3H)-dione
Homo sapiens
at pH 7.5 and 37°C
0.00214
6-chloro-7-(2-hydroxyphenyl)-1-methylpyrido[2,3-d]pyrimidine-2,4(1H,3H)-dione
Rattus norvegicus
at pH 7.5 and 37°C
0.00521
6-chloro-7-(2-hydroxyphenyl)pyrido[2,3-d]pyrimidine-2,4(1H,3H)-dione
Homo sapiens
at pH 7.5 and 37°C
0.1
6-chloro-7-(2-hydroxyphenyl)pyrido[2,3-d]pyrimidine-2,4(1H,3H)-dione
Rattus norvegicus
IC50 above 0.1 mM, at pH 7.5 and 37°C
0.0124
7-methoxy-3-methyl-1-phenyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine
Homo sapiens
at pH 7.5 and 37°C
0.1
7-methoxy-3-methyl-1-phenyl-1H-benzofuro[3,2-b]pyrazolo[4,3-e]pyridine
Rattus norvegicus
IC50 above 0.1 mM, at pH 7.5 and 37°C
0.0421
sulfamic acid
Homo sapiens
-
at pH 7.4 and 37°C
0.0421
sulfamic acid
Homo sapiens
at pH 7.5 and 37°C
0.0773
sulfamic acid
Rattus norvegicus
-
at pH 7.4 and 37°C
0.0773
sulfamic acid
Rattus norvegicus
at pH 7.5 and 37°C
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malfunction
-
cytosolic 5'-nucleotidase cN-II is implicated in drug resistance in patients and dephosphorylates 5-fluoro-dUMP, 3'-azido-3-dTMP, 2'-chloro-2'-deoxyAMP, and related compounds, which represent the monophosphorylated forms of nucleoside prodrugs
malfunction
enzyme deficient mice exhibit decreased callus size after intramembranous bone repair and reduced alkaline phosphatase activity during intramembranous bone healing. Enzyme deficiency leads to decreased osteoclast activity during the remodeling phase of intramembranous bone healing
malfunction
-
enzyme knockdown enhances tumor necrosis factor-stimulated interleukin-8 production
malfunction
the knockdown of enzyme expression in cultured intestinal epithelial cells results in dramatic defects in intraepithelial localization and replication as well as defective transepithelial translocation by Salmonella spp.
metabolism
-
5'-nucleotidases are involved in the purine and pyrimidine nucleotide metabolic pathways, overview. CD73 enzymatic activity is implicated in two major extracellular metabolic pathways with distinct physiological roles, extracellular adenosine nucleotide and NAD+ metabolism
metabolism
-
the key and rate-limiting enzyme in the ectonucleotidase pathway is ecto-5'-nucleotidase, which catalyzes the final step of dephosphorylation of AMP to adenosine
metabolism
the nucleotide salvage pathway is regulated at a molecular level
metabolism
-
Trichomonas foetus is devoid of the ability to synthesize purine nucleotides de novo, depending instead on salvaging purines from the host environment. Ecto-5'-nucleotidase catalyzes the final step of extracellular nucleotide degradation, the hydrolysis of nucleoside 5'-monophosphates to the corresponding nucleosides and phosphate
metabolism
-
the key and rate-limiting enzyme in the ectonucleotidase pathway is ecto-5'-nucleotidase, which catalyzes the final step of dephosphorylation of AMP to adenosine
-
physiological function
-
5'-nucleotidase activities regulate the quantity of nucleotides generated from both de novo and salvage pathways, overview. 5'-Nucleotidase designates a set of enzymes, which catalyze the hydrolysis of ribonucleoside and deoxyribonucleoside monophosphates into the corresponding nucleosides plus phosphate. 5'-Nucleotidases are classified according to subcellular localization, nucleobase specificity and their ability to hydrolyze deoxynucleoside monophosphate substrates. Membrane-bound 5'-nucleotidases are ectoenzymes principally involved in salvage of extracellular nucleosides, and often display a preference toward adenosine monophosphate, thereby modulating signal transduction cascades involving purinergic receptors. Cytosolic 5'-nucleotidases are members of the haloacid dehalogenase superfamily of enzymes, which are two-domain proteins containing a modified Rossman fold as the core and a variable cap structure. Extracellular and intracellular 5'-nucleotidase activities participate in purine and pyrimidine salvage to support balanced synthesis of nucleotides, which is critical for maintaining high fidelity DNA replication. The major physiological roles for CD73 in extracellular adenosine metabolism are severalfold, overview. cNII is subject to complex allosteric regulation
physiological function
-
5'-nucleotidase UshA is required for growth when nucleotides are provided as the sole source of phosphate, a condition that is likely relevant to bacterial growth in the wild
physiological function
cytosolic 5'-nucleotidase II catalyzes the dephosphorylation of 6-hydroxypurine nucleoside 5'-monophosphates and participates in the regulation of purine nucleotide pools within the cell
physiological function
-
ISN1 is responsible for virtually all IMP 5'-nucleotidase activity in budding yeast cells and is required for cells to release inosine into the culture medium
physiological function
-
the ecto-5'-nucleotidase plays a role in the control of extracellular nucleotide concentrations in the cell and is involved in the release of free adenosine into extracellular medium
physiological function
-
the enzyme plays a role in the generation of extracellular adenosine, a key modulator of male fertility
physiological function
-
the enzyme plays a role in the generation of extracellular adenosine, a key modulator of male fertility
physiological function
-
in neonatal blood plasma, soluble 5'-nucleotidase and alkaline phosphatase mediate conversion of AMP to adenosine, whereas soluble adenosine deaminase catabolizes adenosine to inosine. Newborn blood plasma demonstrates substantially higher adenosine-generating 5'-nucleotidase and alkaline phosphatase activity and lower metabolizing adenosine deaminase activity than adult plasma. Supplementation with AMP inhibits whereas selective pharmacologic inhibition of 5'-nucleotidase enhances Toll-like receptor-mediated tumor necrosis factor TNF-alpha production in neonatal whole blood
physiological function
-
the two domains can be expressed individually in Escherichia coli and fold independently. The C-terminal domain, which contains the substrate-binding pocket, is completely inactive while the N-terminal domain with the two-metal-ion-centers and the core catalytic residues exhibits significant activity, especially towards substrates with activated phosphate bonds such as ATP, ADP, 4-nitrophenyl phosphate. The two domains do not reconstitute the full-length protein from the two folded individual domains
physiological function
transgenic mice expressing a dominant active mutant of downstream regulatory element antagonist modulator, daDREAM, show a drastic reduction of the amount of transcript of Ca2+-activated nucleotidase. Ca2+-activated nucleotidase down-regulation is also found in neuroblastoma SH-SY5Y cells stably overexpressing wild type DREAM or daDREAM, thus providing a simple cell model to investigate the protein maturation pathway. The down-regulation of CANT1 is associated with reduced protein secretion and increased degradation rates
physiological function
-
upon blockade of enzyme by concanavalin A, the strength of adhesion to different extracellular matrix proteins is not altered, but at the same time the invasion ability of the cells is decreased. Knocking down the enzyme in melanoma cells does not influence cell invasion but abolishes their migration on tenascin C, indicating that an enzyme-tenascin C complex is involved in cell migration and invasion and thus in the regulation of melanoma progression
physiological function
-
enzyme overexpression increases the abundance of NAD+ and the activation of the sirtuins SIRT1 and SIRT6
physiological function
enzyme overexpression promotes breast cancer cell proliferation by stimulating the AKT/GSK-3beta/beta-catenin signal pathway
physiological function
the enzyme is a Streptococcus pyogenes virulence factor that facilitates immune evasion from the host
physiological function
the enzyme is essential for the production of luminal adenosine in the gut in vivo
physiological function
the enzyme promotes hepatocellular carcinoma growth and metastasis and upregulates the expression of epithelial growth factor receptor in hepatocellular carcinoma
physiological function
the enzyme strongly inhibits ADP- or collagen-induced platelet aggregation
physiological function
-
the enzyme is a Streptococcus pyogenes virulence factor that facilitates immune evasion from the host
-
physiological function
-
the enzyme plays a role in the generation of extracellular adenosine, a key modulator of male fertility
-
physiological function
-
the ecto-5'-nucleotidase plays a role in the control of extracellular nucleotide concentrations in the cell and is involved in the release of free adenosine into extracellular medium
-
additional information
-
CD73 is highly induced by ischemia and hypoxia, and increased expression in cardiac ischemia is mediated by a positive feedback loop involving adenosine
additional information
the enzyme interferes with the phosphorylation-dependent activation of nucleoside analogues used in the treatment of cancer and viral diseases
additional information
-
the enzyme interferes with the phosphorylation-dependent activation of nucleoside analogues used in the treatment of cancer and viral diseases
additional information
-
unilateral cortical stab injury causes a rapid and irreversible loss of the enzyme from neurons, accounting for a decrease in the overall enzyme expression, accompanied with a gradual increase in e-5NT-positive astrocytes, accounting for upregulation of the enzyme levels in the injured area. CSI induces dynamic changes in the expression pattern of e-5NT that modify the ATP/adenosine ratio and the extent of P1 and P2 receptors activation and, therefore, outcome of the pathological processes after cortical stab injury
additional information
-
unilateral cortical stab injury causes a rapid and irreversible loss of the enzyme from neurons, accounting for a decrease in the overall enzyme expression, accompanied with a gradual increase in e-5NT-positive astrocytes, accounting for upregulation of the enzyme levels in the injured area. CSI induces dynamic changes in the expression pattern of e-5NT that modify the ATP/adenosine ratio and the extent of P1 and P2 receptors activation and, therefore, outcome of the pathological processes after cortical stab injury
-
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119000
-
enzyme N-II, gel filtration
132000
-
eNT from lung, SDS-PAGE
135000
-
enzyme from glioblastoma cells, gel filtration in presence of 0.6% sodium deoxycholate
140000 - 150000
-
gel filtration, sucrose density gradient centrifugation
144000
-
eNT from skeletal muscle, kidney, heart and liver, SDS-PAGE
147000
-
heart, membrane-bound, SDS-PAGE after cross-linkage with dimethylsuberimidate
148000
-
1 * 148000 + 1 * 94000, cytsolic enzyme, SDS-PAGE
182000
-
liver cytosol, absence of ATP, allosteric protein, binding of adenine nucleotides induces a more compact and stable conformation, calculation from Stokes radius and partial specific volume
191000
-
liver cytosol, SDS-PAGE after cross-linkage with dimethylsuberimidate
197000
-
liver cytosol, presence of 3 mM ATP, allosteric protein, binding of adenine nucleotides induces a more compact and stable conformation, calculation from Stokes radius and partial specific volume
205000
-
sucrose density gradient centrifugation
230000
-
brain cytoplasm, gel filtration
24300
x * 24300, SDS-PAGE
24500
-
1 * 24500 + 1 * 25500, SDS-PAGE
250000
-
cytosolic enzyme, gel filtration
25500
-
1 * 24500 + 1 * 25500, SDS-PAGE
260000
-
liver microsomes, gel filtration in presence of detergents
26300
-
x * 52400, enzyme from Golgi apparatus, x * 26300, plasma membrane enzyme, SDS-PAGE
265000
-
membrane-bound enzyme, gel filtration
286000
-
enzyme from synaptic plasma membrane, gel filtration
33000
-
3 * 33000, SDS-PAGE
35900
2 * 35900, SDS-PAGE
43000
-
x * 43000, SDS-PAGE
47700
x * 47700, calculation from nucleotide sequence
52000
-
enzyme N-I, gel filtration
52400
-
x * 52400, enzyme from Golgi apparatus, x * 26300, plasma membrane enzyme, SDS-PAGE
53000
-
4 * 53000, cytoplasmic enzyme, SDS-PAGE
53700
-
x * 53700, enzyme from Golgi apparatus, SDS-PAGE
54300
-
4 * 54300, brain cytoplasm, SDS-PAGE
55000
-
x * 55000, plasma membrane enzyme, SDS-PAGE
56000
-
x * 56000, membrane-bound enzyme, deglycosylated, SDS-PAGE
57300
-
4 * 57300, calculated from amino acid sequence
58000
-
x * 67000, glycosylated enzyme form, x * 58000, deglycosylated enzyme form, SDS-PAGE
60000
-
x * 60000, SDS-PAGE
62000
-
2 * 62000, SDS-PAGE
63000
-
x * 63000, SDS-PAGE
64432
x * 64432, calculated
64480
x * 64480, calculated
73000
-
x * 73000, membrane-bound enzyme, SDS-PAGE under reducing conditions
85000
-
x * 92000, glycosylated enzyme form, x * 85000 deglycosylated enzyme form, SDS-PAGE
92000
-
x * 92000, glycosylated enzyme form, x * 85000 deglycosylated enzyme form, SDS-PAGE
94000
-
1 * 148000 + 1 * 94000, cytsolic enzyme, SDS-PAGE
130000
-
gel filtration
130000
-
liver cytosol, gel filtration
131000
-
gel filtration
131000
-
x * 131000, brain cytosol, SDS-PAGE
140000
-
gel filtration in absence of detergent
140000
-
liver plasma membrane, SDS-PAGE after cross-linking with dimethylsuberimidate
150000
-
-
268000
-
brain cytosol, gel filtration
268000
-
enzyme from glioblastoma cells, gel filtration in presence of 0.1% Triton X-100
51000
-
4 * 51000, SDS-PAGE
51000
-
x * 74000, x * 68000, X * 51000, SDS-PAGE
57000
-
SDS-PAGE
57000
-
1 * 65000 + 1 * 57000, liver cytosol, SDS-PAGE
65000
gel filtration
65000
-
1 * 65000 + 1 * 57000, liver cytosol, SDS-PAGE
65000
x * 65000, truncated form of enzyme
66000
-
1 * 66000, SDS-PAGE
66000
-
2 * 66000, eNT from lung, SDS-PAGE
66200
-
x * 66200, calculation from nucleotide sequence
66200
-
x * 66200, SDS-PAGE
67000
x * 67000, SDS-PAGE
67000
-
4 * 67000, SDS-PAGE
67000
-
4 * 67000, membrane-bound enzyme, SDS-PAGE
67000
-
x * 67000, glycosylated enzyme form, x * 58000, deglycosylated enzyme form, SDS-PAGE
68000
-
x * 74000, x * 68000, X * 51000, SDS-PAGE
68000
-
2 * 68000, the subunits in eNT dimers are not linked by disulfide bridges, but by non-covalent bonds. Dissociation precedes inactivation and unfolding, SDS-PAGE
70000
-
SDS-PAGE
70000
-
x * 70000, SDS-PAGE
70000
-
2 * 70000, liver plasma membrane, SDS-PAGE
71000
-
homodimeric CD73
71000
-
x * 71000, SDS-PAGE
72000
gel filtration
72000
-
x * 72000, SDS-PAGE
72000
-
x * 72000, SDS-PAGE
72000
-
2 * 72000, dimeric structure in absence of detergents, SDS-PAGE
72000
-
4 * 72000, liver microsomes, tetrameric structure in presence of detergents, SDS-PAGE
72000
-
x * 72000, synaptic plasma membrane, SDS-PAGE
72000
-
2 * 72000, eNT from skeletal muscle, kidney, heart and liver, SDS-PAGE
74000
-
2 * 74000, enzyme from glioblastoma cells may have dimeric or tetrameric structure
74000
-
2 * 74000, heart, membrane-bound, SDS-PAGE
74000
-
4 * 74000, enzyme from glioblastoma cells may have dimeric or tetrameric structure
74000
-
x * 74000, x * 68000, X * 51000, SDS-PAGE
74000
-
x * 74000, microsomal enzyme, SDS-PAGE
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homotetramer
-
4 * 57300, calculated from amino acid sequence
homotrimer
-
3 * 33000, SDS-PAGE
?
-
x * 52400, enzyme from Golgi apparatus, x * 26300, plasma membrane enzyme, SDS-PAGE
?
-
x * 53700, enzyme from Golgi apparatus, SDS-PAGE
?
-
x * 55000, plasma membrane enzyme, SDS-PAGE
?
-
x * 66200, calculation from nucleotide sequence
?
-
x * 56000, membrane-bound enzyme, deglycosylated, SDS-PAGE
?
-
x * 67000, glycosylated enzyme form, x * 58000, deglycosylated enzyme form, SDS-PAGE
?
-
x * 73000, membrane-bound enzyme, SDS-PAGE under reducing conditions
?
-
x * 74000, microsomal enzyme, SDS-PAGE
?
x * 47700, calculation from nucleotide sequence
?
x * 65000, truncated form of enzyme
?
-
x * 74000, x * 68000, X * 51000, SDS-PAGE
?
-
x * 92000, glycosylated enzyme form, x * 85000 deglycosylated enzyme form, SDS-PAGE
?
-
x * 72000, synaptic plasma membrane, SDS-PAGE
?
-
x * 131000, brain cytosol, SDS-PAGE
?
x * 70000, recombinant enzyme, SDS-PAGE
?
x * 69870, recombinant enzyme, calculated from amino acid sequence
?
-
x * 70000, recombinant enzyme, SDS-PAGE
-
?
-
x * 69870, recombinant enzyme, calculated from amino acid sequence
-
dimer
-
2 * 70000, liver plasma membrane, SDS-PAGE
dimer
-
1 * 65000 + 1 * 57000, liver cytosol, SDS-PAGE
dimer
-
2 * 68000, the subunits in eNT dimers are not linked by disulfide bridges, but by non-covalent bonds. Dissociation precedes inactivation and unfolding, SDS-PAGE
dimer
-
the active and the inactive enzyme species occur as amphiphilic dimers
dimer
-
2 * 66000, eNT from lung, SDS-PAGE
dimer
-
2 * 72000, eNT from skeletal muscle, kidney, heart and liver, SDS-PAGE
dimer
-
2 * 74000, enzyme from glioblastoma cells may have dimeric or tetrameric structure
dimer
-
2 * 74000, heart, membrane-bound, SDS-PAGE
dimer
-
2 * 72000, dimeric structure in absence of detergents, SDS-PAGE
dimer
-
1 * 148000 + 1 * 94000, cytsolic enzyme, SDS-PAGE
dimer
-
2 * 62000, SDS-PAGE
dimer
2 * 35900, SDS-PAGE
dimer
-
2 * 35900, SDS-PAGE
-
dimer
-
1 * 24500 + 1 * 25500, SDS-PAGE
homodimer
2 * 29200, His6-tagged enzyme, calculated from amino acid sequence
homodimer
2 * 30000, His6-tagged enzyme, SDS-PAGE
homodimer
-
2 * 29200, His6-tagged enzyme, calculated from amino acid sequence
-
homodimer
-
2 * 30000, His6-tagged enzyme, SDS-PAGE
-
homodimer
2 * 60000, SDS-PAGE
monomer
-
1 * 66000, SDS-PAGE
monomer
-
1 * 110000-120000, SDS-PAGE, inconsistency with value determined by gel filtration due to glycoprotein nature of the enzyme
oligomer
at least four identical subunits in solution
oligomer
three or more subunits in solution
tetramer
the active tetramer is formed by interaction between two identical dimers via interface B: this interface contains 28 aminoacid residues, of which 8 make hydrogen bonds
tetramer
-
4 * 67000, SDS-PAGE
tetramer
-
4 * 51000, SDS-PAGE
tetramer
-
4 * 53000, cytoplasmic enzyme, SDS-PAGE
tetramer
-
x-ray crystallography
tetramer
cN-II functions as a tetramer forming a dimer of dimers, structural basis for the allosteric regulation and substrate recognition of cytosolic 5-nucleotidase II, overview. The effector binding site, corresponding to effector site 1, is located close to one of the subunit interfaces where the effector takes part in subunit-subunit interactions by forming electrostatic interactions between the effector phosphates and the positively charged residues of each subunit
tetramer
-
cytosolic 5'-nucleotidase II, cNII, is a tetramer, formed from two homodimers
tetramer
-
4 * 72000, liver microsomes, tetrameric structure in presence of detergents, SDS-PAGE
tetramer
-
4 * 54300, brain cytoplasm, SDS-PAGE
tetramer
-
4 * 74000, enzyme from glioblastoma cells may have dimeric or tetrameric structure
tetramer
-
4 * 50000-52000, liver cytosol, SDS-PAGE
tetramer
-
4 * 67000, membrane-bound enzyme, SDS-PAGE
additional information
cN-II is a relatively large enzyme of the structural HAD superfamily, which contains, besides the alpha/beta-Rossmann fold and the four-helix bundle, non-conserved regions involved in effector binding and subunit-subunit interactions
additional information
-
cN-II is a relatively large enzyme of the structural HAD superfamily, which contains, besides the alpha/beta-Rossmann fold and the four-helix bundle, non-conserved regions involved in effector binding and subunit-subunit interactions
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