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Literature summary for 3.1.3.5 extracted from

  • Wada, I.; Eto, S.; Himeno, M.; Kato, K.
    5'-Nucleotidase in rat liver lysosomes (1987), J. Biochem., 101, 1077-1085.
    View publication on PubMed
Search for more literature for 3.1.3.5

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Rattus norvegicus 5764
-
plasma membrane ectoenzyme with the catalytic site facing outward Rattus norvegicus 5886
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
 2 * 72000, dimeric structure in absence of detergents, SDS-PAGE Rattus norvegicus
72000
-
 4 * 72000, liver microsomes, tetrameric structure in presence of detergents, SDS-PAGE Rattus norvegicus
140000
-
 gel filtration in absence of detergent Rattus norvegicus
260000
-
 liver microsomes, gel filtration in presence of detergents Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver membrane-bound Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-AMP + H2O
-
 Rattus norvegicus adenosine + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 72000, dimeric structure in absence of detergents, SDS-PAGE Rattus norvegicus
tetramer 4 * 72000, liver microsomes, tetrameric structure in presence of detergents, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 unstable at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Rattus norvegicus