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Literature summary for 3.1.3.5 extracted from
- Purification and properties of a novel nucleotide-hydrolysing enzyme (5'-nucleotidase) from Boophilus microplus (1989), Biochem. J., 258, 79-85.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cl- | weak inhibition | Rhipicephalus microplus |  |
| concanavalin A | strong inhibition | Rhipicephalus microplus | |
| EDTA | strong inhibition | Rhipicephalus microplus | |
| PO43- | weak inhibition | Rhipicephalus microplus | |
| SO42- | weak inhibition | Rhipicephalus microplus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00009 | - |
ADP | - |
Rhipicephalus microplus | |
| 0.0001 | - |
ATP | - |
Rhipicephalus microplus | |
| 0.0003 | - |
5'-AMP | - |
Rhipicephalus microplus | |
| 0.28 | - |
bis-p-nitrophenyl phosphate | - |
Rhipicephalus microplus | |
| 0.87 | - |
p-Nitrophenyl phenylphosphonate | - |
Rhipicephalus microplus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plasma membrane | - |
Rhipicephalus microplus | 5886 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhipicephalus microplus | - |
tick | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhipicephalus microplus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 38.8 | - |
- |
Rhipicephalus microplus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-AMP + H2O | - |
Rhipicephalus microplus | adenosine + phosphate | - |
? | |
| ADP + H2O | - |
Rhipicephalus microplus | adenosine + diphosphate | - |
? | |
| ATP + H2O | - |
Rhipicephalus microplus | adenosine + triphosphate | - |
? | |
| bis-p-nitrophenylphosphate + H2O | - |
Rhipicephalus microplus | ? | - |
? | |
| p-nitrophenyl phenylphosphonate + H2O | - |
Rhipicephalus microplus | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 7.4 | - |
Rhipicephalus microplus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | 8.5 | - |
Rhipicephalus microplus |
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