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Literature summary for 3.1.3.5 extracted from

  • Tkacz-Stachowska, K.; Lechward, K.; Skladanowski, A.C.
    Isolation and characterization of pigeon breast muscle cytosolic 5'-nucleotidase-I (cN-I) (2005), Acta Biochim. Pol., 52, 789-796.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ADP strong, allosteric activiator Columba livia

Inhibitors

Inhibitors Comment Organism Structure
phosphate
-
Columba livia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
5'-dCMP 37°C, pH 7.0, presence of 1 mM ADP Columba livia
0.29
-
5'-AMP 37°C, pH 7.0, presence of 1 mM ADP Columba livia
0.75
-
5'-dCMP 37°C, pH 7.0 Columba livia
1.67
-
5'-IMP 37°C, pH 7.0, presence of 1 mM ADP Columba livia
1.85
-
5'-AMP 37°C, pH 7.0 Columba livia
2 3 5'-dAMP 37°C, pH 7.0 Columba livia
5.2
-
5'-dAMP 37°C, pH 7.0, presence of 1 mM ADP Columba livia
10.8
-
5'-IMP 37°C, pH 7.0 Columba livia

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Columba livia 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
x * 43000, SDS-PAGE Columba livia

Organism

Organism UniProt Comment Textmining
Columba livia
-
isoform cN-I
-

Purification (Commentary)

Purification (Comment) Organism
-
Columba livia

Source Tissue

Source Tissue Comment Organism Textmining
muscle breast muscle Columba livia
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.33
-
37°C, pH 7.0 Columba livia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-AMP + H2O absence of ADP, 60% loss of activity Columba livia adenosine + phosphate
-
?
5'-dAMP + H2O presence of ADP, 66% of the activity with 5’-AMP Columba livia deoxyadenosine + phosphate
-
?
5'-dCMP + H2O presence of ADP, 16% of the activity with 5’-AMP Columba livia deoxycytidine + phosphate
-
?
5'-dIMP + H2O presence of ADP, 38% of the activity with 5’-AMP Columba livia deoxyinosine + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Columba livia