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Literature summary for 3.1.3.5 extracted from

  • Gounaris, K.; Selkirk, M.E.; Sadeghi, S.J.
    A nucleotidase with unique catalytic properties is secreted by Trichinella spiralis (2004), Mol. Biochem. Parasitol., 136, 257-264.
    View publication on PubMed
Search for more literature for 3.1.3.5

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol stimulation Trichinella spiralis
glutathione stimulation Trichinella spiralis

Cloned(Commentary)

Cloned (Comment) Organism
-
 Trichinella spiralis

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 5 mM, inhibition of both hydrolysis of AMP and ADP Trichinella spiralis
Zn2+ 5 mM, inhibition of both hydrolysis of AMP and ADP Trichinella spiralis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.162
-
 5'-AMP pH 7.5 Trichinella spiralis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ stimulation Trichinella spiralis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
 x * 67000, SDS-PAGE Trichinella spiralis

Organism

Organism UniProt Comment Textmining
Trichinella spiralis Q8MQS9 enzyme has both Mg2+-dependent diphosphohydrolyse/apyrase and 5’-nucleotidase activity
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information sequence contains an N-terminal signal peptide, but lacks a C-terminal signature sequence for addition of a glycosyl phosphatidylinositol anchor Trichinella spiralis

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Trichinella spiralis

Source Tissue

Source Tissue Comment Organism Textmining
culture fluid
-
 Trichinella spiralis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-AMP + H2O enzyme is monospecific for AMP among nucleoside 5’-monophosphates Trichinella spiralis adenosine + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 67000, SDS-PAGE Trichinella spiralis