Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.5 extracted from

  • Carter, S.G.; Tipton, C.L.
    Purification and characterization of a 5'-nucleotidase from Zea mays microsomes (1986), Phytochemistry, 25, 33-37.
No PubMed abstract available
Search for more literature for 3.1.3.5

Inhibitors

Inhibitors Comment Organism Structure
6-Chloropurine riboside
-
 Zea mays
6-gamma,gamma-dimethylallylaminopurine riboside
-
 Zea mays
6-mercaptopurine riboside
-
 Zea mays
adenosine
-
 Zea mays
adenosine 5'-sulfate
-
 Zea mays
ATP
-
 Zea mays
cAMP
-
 Zea mays
kinstin riboside
-
 Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0571
-
 5'-GMP
-
 Zea mays
0.0577
-
 5'-AMP
-
 Zea mays
0.0816
-
 5'-IMP
-
 Zea mays
0.2
-
 5'-UMP
-
 Zea mays
0.3333
-
 5'-CMP
-
 Zea mays

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Zea mays
-
-
plasma membrane
-
 Zea mays 5886
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24500
-
 1 * 24500 + 1 * 25500, SDS-PAGE Zea mays
25500
-
 1 * 24500 + 1 * 25500, SDS-PAGE Zea mays
49000
-
 gel filtration Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification 40% carbohydrate Zea mays

Purification (Commentary)

Purification (Comment) Organism
-
 Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Zea mays
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.73
-
-
 Zea mays

Storage Stability

Storage Stability Organism
-20°C, several months Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-AMP + H2O
-
 Zea mays adenosine + phosphate
-
 ?
5'-CMP + H2O 116% of the activity with 5'-AMP Zea mays cytidine + phosphate
-
 ?
5'-CMP + H2O 46% of the activity with 5'-AMP Zea mays cytidine + phosphate
-
 ?
5'-dIMP + H2O
-
 Zea mays deoxyinosine + phosphate
-
 ?
5'-GMP + H2O 116% of the activity with 5'-AMP Zea mays guanosine + phosphate
-
 ?
5'-UMP + H2O 71% of the activity with 5'-AMP Zea mays uridine + phosphate
-
 ?
5'-UMP + H2O 137% of the activity with 5'-AMP Zea mays uridine + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 24500 + 1 * 25500, SDS-PAGE Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 no clear optimum Zea mays