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2.7.6.1: ribose-phosphate diphosphokinase

This is an abbreviated version!
For detailed information about ribose-phosphate diphosphokinase, go to the full flat file.

Word Map on EC 2.7.6.1

Reaction

ATP
+
D-ribose 5-phosphate
=
AMP
 +
5-phospho-alpha-D-ribose 1-diphosphate

Synonyms

5-phospho-alpha D-ribosyl 1-diphosphate synthase, 5-phosphoribose pyrophosphorylase, 5-phosphoribosyl-1-alpha-diphosphate synthase, 5-phosphoribosyl-1-pyrophosphate synthetase, 5-phosphoribosyl-alpha-1-pyrophosphate synthetase, AN6711.2, ATP: D-ribose-5-phosphate pyrophosphotransferase, ATP:D-ribose-5-phosphate pyrophosphotransferase, bsPRS, Pcal_1127, phosphoribosyl diphosphate synthase, phosphoribosyl pyrophosphate synthase 1 (PRS-1), phosphoribosyl pyrophosphate synthase enzyme, phosphoribosyl pyrophosphate synthetase, phosphoribosyl-1-pyrophosphate synthetase, phosphoribosyl-diphosphate synthetase, phosphoribosylpyrophosphate synthase, phosphoribosylpyrophosphate synthetase, phosphoribosylpyrophosphate synthetase 1, phosphoribosylpyrophosphate synthetase subunit 1, PP-ribose P synthetase, PPRibP synthetase, PRibPP synthase, PRPP synthase, PRPP synthetase, PRPPase, PRPPS, PRPPS1, PRPPS2, PRPS1, Prps1a, Prps1b, PRS, PRS-I, PRS1, Prs4, PrsA, PrsB, PrsC, pyrophosphokinase, ribose phosphate, pyrophosphoribosylphosphate synthetase, ribophosphate pyrophosphokinase, ribose-5-phosphate pyrophosphokinase, ribose-phosphate pyrophosphokinase, ribose-phosphate pyrophosphokinase 1, RPPK, SSO1045, TT_C1184, TT_C1274

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.6 Diphosphotransferases
                2.7.6.1 ribose-phosphate diphosphokinase

Storage Stability

Storage Stability on EC 2.7.6.1 - ribose-phosphate diphosphokinase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 50 mM potassium phosphate buffer, pH 7.5 after rapid freezing in CO2-acetone, quite stable
-
-20ºC, Tris/HCl buffer 50 mM, pH 7.6, 50% glycerol, stable
-
-70ºC, glycerol 10%, stable
-
-80°C, 50 mM Tris-HCl (pH 7.5), up to 7 months, no loss of activity
-
-80ºC, potassium phosphate 50 mM, pH 7.4
-
-80ºC, potassium phosphate buffer, pH 7.4, stable
-
0°C or -10°C, 50 mM potassium phosphate buffer, pH 7.4, stable for weeks
-
4°C or -20°C in 25 mM Tris–HCl (pH 7.6), 50% (v/v) glycerol, purified enzyme is stable for at least 90 days when stored at
4°C, 50% loss of activity after 2 months. -20ºC, rapid loss of activity
-
4ºC, 50% loss of activity after 2 months. -20ºC, more rapid loss of activity. Presence of substrates enhances stability during storage
-
4ºC, without stabilizing agents, half-life of inactivation of 21, 8 and 1 hours for native enzyme, isoenzymes rPRSI and rPRSII, respectively
-
stored and diluted in the presence of phosphate 50 mM to maintain stability. With 2 mM MgATP2- or more, the enzyme is fully stable upon dilution and subsequent incubation at 37ºC
-