Literature summary for 2.7.6.1 extracted from

Ishijima, S.; Kita, K.; Ahmad, I.; Ishizuka, T.; Taira, M.; Tatibana, M.
Expression of rat phosphoribosylpyrophosphate synthetase subunits I and II in Escherichia coli. Isolation and characterization of the recombinant isoforms (1991), J. Biol. Chem., 266, 15693-15697.

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Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	at 50 and 100 mM, KCl, KHCO3 and CH3COOK have no activating effects	Rattus norvegicus
phosphate	requirement	Rattus norvegicus
phosphate	no activity at phosphate concentrations lower than 0.4 mM	Rattus norvegicus
sulfate	can partially replace phosphate in activation	Rattus norvegicus

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Rattus norvegicus

Inhibitors

Inhibitors	Comment	Organism	Structure
ADP	one of the most effective	Rattus norvegicus
AMP	one of the most effective	Rattus norvegicus
CTP	no inhibition	Rattus norvegicus
GDP	one of the most effective	Rattus norvegicus
GMP	no inhibition	Rattus norvegicus
GTP	no inhibition	Rattus norvegicus
UDP	no inhibition	Rattus norvegicus
UMP	no inhibition	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.04	-	D-ribose 5-phosphate	pH 7.4, 37ºC, isoenzyme PRSI	Rattus norvegicus
0.044	-	ATP	pH 7.4, 37ºC, isoenzyme PRSI	Rattus norvegicus
0.06	-	ATP	pH 7.4, 37ºC, isoenzyme PRSII	Rattus norvegicus
0.073	-	D-ribose 5-phosphate	pH 7.4, 37ºC, isoenzyme PRSII	Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
34000	-	x * 34000, SDS-PAGE, cloned and purified isoenzymes PRSI and PRSII	Rattus norvegicus
550000	-	gel filtration, cloned and purified rPRSII	Rattus norvegicus
700000	1200000	gel filtration, cloned and purified rPRSI	Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + D-ribose 5-phosphate	Rattus norvegicus	the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan	AMP + 5-phospho-alpha-D-ribose 1-diphosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	rat	-

Purification (Commentary)

Purification (Comment)	Organism
polyethylene glycol, acid precipitation and chromatography on DEAE-5PW HPLC, purification of the two isoforms PRSI and PRSII	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
25.7	-	isoenzyme PRSI	Rattus norvegicus
34.5	-	isoenzyme PRSII	Rattus norvegicus

Storage Stability

Storage Stability	Organism
-80ºC, potassium phosphate 50 mM, pH 7.4	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + D-ribose 5-phosphate	-	Rattus norvegicus	AMP + 5-phospho-alpha-D-ribose 1-diphosphate	-	r
ATP + D-ribose 5-phosphate	the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan	Rattus norvegicus	AMP + 5-phospho-alpha-D-ribose 1-diphosphate	-	r

Subunits

Subunits	Comment	Organism
?	x * 34000, SDS-PAGE, cloned and purified isoenzymes PRSI and PRSII	Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
49	-	half-life of 0.5 min and 90 min for isoenzymes rPRSII and rPRSI, respectively	Rattus norvegicus

pI Value

Organism	Comment	pI Value Maximum	pI Value
Rattus norvegicus	isoenzyme rPRSII	4.4	4.3
Rattus norvegicus	isoenzyme rPRSI	-	4.4

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Release 2023.1 (January 2023)