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2.7.6.1: ribose-phosphate diphosphokinase

This is an abbreviated version!
For detailed information about ribose-phosphate diphosphokinase, go to the full flat file.

Word Map on EC 2.7.6.1

Reaction

ATP
+
D-ribose 5-phosphate
=
AMP
 +
5-phospho-alpha-D-ribose 1-diphosphate

Synonyms

5-phospho-alpha D-ribosyl 1-diphosphate synthase, 5-phosphoribose pyrophosphorylase, 5-phosphoribosyl-1-alpha-diphosphate synthase, 5-phosphoribosyl-1-pyrophosphate synthetase, 5-phosphoribosyl-alpha-1-pyrophosphate synthetase, AN6711.2, ATP: D-ribose-5-phosphate pyrophosphotransferase, ATP:D-ribose-5-phosphate pyrophosphotransferase, bsPRS, Pcal_1127, phosphoribosyl diphosphate synthase, phosphoribosyl pyrophosphate synthase 1 (PRS-1), phosphoribosyl pyrophosphate synthase enzyme, phosphoribosyl pyrophosphate synthetase, phosphoribosyl-1-pyrophosphate synthetase, phosphoribosyl-diphosphate synthetase, phosphoribosylpyrophosphate synthase, phosphoribosylpyrophosphate synthetase, phosphoribosylpyrophosphate synthetase 1, phosphoribosylpyrophosphate synthetase subunit 1, PP-ribose P synthetase, PPRibP synthetase, PRibPP synthase, PRPP synthase, PRPP synthetase, PRPPase, PRPPS, PRPPS1, PRPPS2, PRPS1, Prps1a, Prps1b, PRS, PRS-I, PRS1, Prs4, PrsA, PrsB, PrsC, pyrophosphokinase, ribose phosphate, pyrophosphoribosylphosphate synthetase, ribophosphate pyrophosphokinase, ribose-5-phosphate pyrophosphokinase, ribose-phosphate pyrophosphokinase, ribose-phosphate pyrophosphokinase 1, RPPK, SSO1045, TT_C1184, TT_C1274

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.6 Diphosphotransferases
                2.7.6.1 ribose-phosphate diphosphokinase

Purification

Purification on EC 2.7.6.1 - ribose-phosphate diphosphokinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
4500fold purification
-
acetone powder, acid and streptomycin precipitation, ammonium sulfate fractionation, heat treatment and agarose gel filtration, 3400fold purification, presence of substrates enhances stability during purification
-
acetone powder, acid and streptomycin precipitation, heat treatment and agarose chromatography, 1500 to 3000fold purification
-
ammonium sulfate and isoelectric precipitations, purified to near homogeneity
-
ammonium sulfate and polyethylene glycol precipitation, affinity chromatography and anion exchange chromatography, purification of isoenzyme 3
-
ammonium sulfate fractionation, chromatography on DEAE-cellulose and ultrafiltration
-
ammonium sulfate fractionation, chromatography on Sephacryl S300 HR and on Protein-Pak DEAE 40 HR and affinity chromatography
-
ammonium sulfate precipitation, Q-Sepharose column chromatography, Superdex 200 gel filtration, and Mono Q column chromatography
-
extraction, streptomycin-heat treatment, ammonium sulfate and acid precipitation, 450fold purification
-
extraction, streptomycin-heat treatment, ammonium sulfate and acid precipitation, affinity chromatography
-
gel filtration
-
His-Trap HP column chromatography and Superdex 200 gel filtration
-
HisTrap column chromatography and Sephadex G-25 gel filtration
Ni2+ resin column chromatography
-
Ni2+-NTA agarose column chromatography
-
partial, 400fold purification
-
polyethylene glycol and acid precipitation
-
polyethylene glycol precipitation, hydroxyapatite fractionation, gel filtration on Toyopearl HW-65F, chromatography on DEAE-Toyopearl 650S and gel filtration
-
polyethylene glycol, acid precipitation and chromatography on DEAE-5PW HPLC, purification of the two isoforms PRSI and PRSII
-
recombinant
-
recombinant enzyme 4.6fold from Escherichia coli strain BL21-CodonPlus(DE3)-RIL by heat treatment at 80°C for 25 min, anion exchange chromatography, dialysis, and another step of anion exchange chromatography
recombinant His-tagged PRS1 from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography
recombinant protein
recombinant protein, using affinity and gel-filtration chromatography
streptomycin precipitation and ammonium sulfate fractionation for isoenzyme PRSI and polyethylene glycol, acid and ammonium sulfate precipitation for isoenzyme PRSII
-
streptomycin sulfate and ammonium sulfate precipitation, chromatography on Dyematrex Gel Blue B and DE52
-
streptomycin-heat treatment, ammonium sulfate fractionation, chromatography on DEAE-Sepharose and affinity chromatography
-
to near homogeneity. Use of Mg2+, ATP and sulfhydryl compounds to stabilize the enzyme permit its isolation from erythrocytes
-