Activating Compound | Comment | Organism | Structure |
---|---|---|---|
1-Aminoethylphosphonate | 0.1 M, 13% of the activity with phosphate | Salmonella enterica subsp. enterica serovar Typhimurium | |
arsenate | 0.1 M, 43% of the activity with phosphate | Salmonella enterica subsp. enterica serovar Typhimurium | |
phosphate | specific and absolute requirement | Salmonella enterica subsp. enterica serovar Typhimurium | |
phosphate | appears to have an effect on the stability of the enzyme as well as a specific catalytic effect | Salmonella enterica subsp. enterica serovar Typhimurium | |
phosphate | the enzyme has 2 sites for phosphate, one of high affinity and one of lower affinity | Salmonella enterica subsp. enterica serovar Typhimurium |
General Stability | Organism |
---|---|
completely inactivated when phosphate is removed by dialysis | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | free form, slight inhibition at 1 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
GSH | marked inhibition of enzyme in crude extract at 1 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
additional information | GSH, 2-mercaptoethanol, 2,3-dimercaptopropanol, dithiothreitol, at 1 mM, have no effect or slightly inhibit the highly purified enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | 0.13 | D-ribose 5-phosphate | pH 7.5, 37ºC | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.06 | - |
ATP | pH 7.5, 37ºC, excess of Mn2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.06 | - |
ATP | ATP in form of MnATP2- | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.1 | - |
ATP | pH 7.5, 37ºC, excess of Mg2+, bimodal kinetic behavior | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.1 | - |
ATP | ATP in form of MgATP2- | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.15 | 0.4 | AMP | pH 7.5, 37ºC | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.35 | - |
ATP | pH 7.5, 37ºC, no excess of Mn2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.35 | - |
ATP | ATP in form of MnATP2- | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.58 | - |
ATP | pH 7.5, 37ºC, excess of Mg2+, bimodal kinetic behavior | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.58 | - |
ATP | ATP in form of MgATP2- | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.3 | - |
phosphate | pH 7.5, 37ºC, high affinity site | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.9 | - |
ATP | pH 7.5, 37ºC, no excess of Mg2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.9 | - |
ATP | ATP in form of MgATP2- | Salmonella enterica subsp. enterica serovar Typhimurium | |
40 | - |
phosphate | pH 7.5, 37ºC, low affinity site | Salmonella enterica subsp. enterica serovar Typhimurium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | no activity observed | Salmonella enterica subsp. enterica serovar Typhimurium | |
Mg2+ | requirement | Salmonella enterica subsp. enterica serovar Typhimurium | |
Mg2+ | required to form a complex with ATP and as a free cation | Salmonella enterica subsp. enterica serovar Typhimurium | |
Mn2+ | equally effective as Mg2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
additional information | absolute requirement for a divalent cation for activity | Salmonella enterica subsp. enterica serovar Typhimurium | |
additional information | no activity observed with Ca2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
Zn2+ | 25% of the activity with Mg2+ | Salmonella enterica subsp. enterica serovar Typhimurium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
540000 | - |
sucrose density gradient sedimentation | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose 5-phosphate | Salmonella enterica subsp. enterica serovar Typhimurium | the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
LT-2 | - |
Purification (Comment) | Organism |
---|---|
extraction, streptomycin-heat treatment, ammonium sulfate and acid precipitation, 450fold purification | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
26.8 U/mg protein, purified enzyme, 400fold to 450fold the activity of crude extract | Salmonella enterica subsp. enterica serovar Typhimurium |
Storage Stability | Organism |
---|---|
0°C or -10°C, 50 mM potassium phosphate buffer, pH 7.4, stable for weeks | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose 5-phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
ATP + D-ribose 5-phosphate | the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan | Salmonella enterica subsp. enterica serovar Typhimurium | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.1 | 8.6 | for either the Mg2+ or the Mn2+ supported reaction | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | very low activity below and above | Salmonella enterica subsp. enterica serovar Typhimurium |