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Literature summary for 2.7.6.1 extracted from
- Class II recombinant phosphoribosyl diphosphate synthase from spinach. Phosphate independence and diphosphoryl donor specificity (2001), J. Biol. Chem., 276, 17851-17856.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | isoenzyme 3 is independent of phosphate | Spinacia oleracea |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of isoenzyme 3 in Escherichia coli | Spinacia oleracea |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-phospho-alpha-D-ribose 1-diphosphate | competitive with respect to ATP and noncompetitive with respect to D-ribose 5-phosphate | Spinacia oleracea | |
| ADP | - |
Spinacia oleracea | |
| AMP | noncompetitive to both substrates | Spinacia oleracea | |
| GDP | - |
Spinacia oleracea | |
| GTP | substrate inhibition | Spinacia oleracea | |
| L-histidine | no inhibition | Spinacia oleracea | |
| L-tryptophan | no inhibition | Spinacia oleracea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
D-ribose 5-phosphate | pH 7.6, 37ºC, isoenzyme 3 | Spinacia oleracea | |
| 0.116 | - |
CTP | pH 7.6, 37ºC, isoenzyme 3 | Spinacia oleracea | |
| 0.137 | - |
UTP | pH 7.6, 37ºC, isoenzyme 3 | Spinacia oleracea | |
| 0.17 | - |
ATP | pH 7.6, 37ºC, isoenzyme 3 | Spinacia oleracea | |
| 0.233 | - |
dATP | pH 7.6, 37ºC, isoenzyme 3 | Spinacia oleracea | |
| 0.65 | - |
GTP | pH 7.6, 37ºC, isoenzyme 3 | Spinacia oleracea | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | no activity observed | Spinacia oleracea | |
| Co2+ | no activity observed | Spinacia oleracea | |
| Mg2+ | requirement | Spinacia oleracea | |
| Mg2+ | required to form a complex with ATP and as a free cation | Spinacia oleracea | |
| Mn2+ | can partially replace Mg2+ | Spinacia oleracea | |
| additional information | no activity observed with Ca2+ | Spinacia oleracea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-ribose 5-phosphate | Spinacia oleracea | - |
AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
spinach | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate and polyethylene glycol precipitation, affinity chromatography and anion exchange chromatography, purification of isoenzyme 3 | Spinacia oleracea |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate | ordered bi-bi reaction | Spinacia oleracea | |
| ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate | sequential kinetic mechanism | Spinacia oleracea |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13.1 | - |
- |
Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-ribose 5-phosphate | - |
Spinacia oleracea | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
| CTP + D-ribose 5-phosphate | less effective than ATP | Spinacia oleracea | CMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
| dATP + D-ribose 5-phosphate | equally effective as ATP | Spinacia oleracea | dAMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
| GTP + D-ribose 5-phosphate | less effective than ATP | Spinacia oleracea | GMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
| additional information | 2-deoxyribose-5-phosphate is not substrate | Spinacia oleracea | ? | - |
? | |
| UTP + D-ribose 5-phosphate | less effective than ATP | Spinacia oleracea | UMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Spinacia oleracea |
| 40 | - |
- |
Spinacia oleracea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
activity increases up to pH 7.2 and decreases above pH 7.6 | Spinacia oleracea |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.45 | - |
AMP | pH 7.6, 37ºC, 5-phospho-alpha-D-ribose 1-diphosphate as substrate, isoenzyme 3 | Spinacia oleracea | |
| 0.52 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.6, 37ºC, ATP as substrate, isoenzyme 3 | Spinacia oleracea | |
| 0.68 | - |
ADP | pH 7.6, 37ºC, ATP as substrate, isoenzyme 3 | Spinacia oleracea | |
| 0.82 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.6, 37ºC, 5-phospho-alpha-D-ribose 1-diphosphate as substrate, isoenzyme 3 | Spinacia oleracea | |
| 1.22 | - |
ADP | pH 7.6, 37ºC, 5-phospho-alpha-D-ribose 1-diphosphate as substrate, isoenzyme 3 | Spinacia oleracea | |
| 1.62 | - |
AMP | pH 7.6, 37ºC, ATP as substrate, isoenzyme 3 | Spinacia oleracea | |
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