Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphate | specific and kinetically complex requirement, 25-50 mM required for optimal 5-phospho-alpha-D-ribose 1-diphosphate synthesis, 5 mM optimal for reverse reaction | Salmonella enterica subsp. enterica serovar Typhimurium |
General Stability | Organism |
---|---|
repeated freezing and thawing slowly denatures the enzyme. Irreversible denaturation by reduction of the phosphate concentration to less than 5 mM, requires 25 mM phosphate or higher for complete stability. Mg2+ ions and ATP stabilize the enzyme but will not replace phosphate | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | the most effective | Salmonella enterica subsp. enterica serovar Typhimurium | |
alpha,beta-methyleneATP | competitive inhibition to ATP | Salmonella enterica subsp. enterica serovar Typhimurium | |
Ca2+ | IC50 of 0.05 mM; inhibition even in presence of excess Mg2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
Cd2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
Co2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
Cu2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
D-ribose 5-phosphate | substrate inhibition in presence of ADP, not in its absence | Salmonella enterica subsp. enterica serovar Typhimurium | |
DL-1,4-Anhydroribitol 5-phosphate | competitive inhibition to D-ribose-5-phosphate | Salmonella enterica subsp. enterica serovar Typhimurium | |
additional information | Ba2+, Fe2+, Mn2+ and Zn2+ do not inhibit at concentrations below 1 mM; purines are more effective than pyrimidines, nucleoside tri and diphosphates are more effective than monophosphates, ribonucleotides are more effective than deoxynucleotides | Salmonella enterica subsp. enterica serovar Typhimurium | |
Ni2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
nucleotides | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.046 | - |
ATP | pH and temperature conditions not mentioned | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.046 | - |
ATP | ATP in form of MgATP2- | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.16 | - |
D-ribose 5-phosphate | pH and temperature conditions not mentioned | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.29 | - |
5-phospho-D-ribose diphosphate | pH and temperature conditions not mentioned | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.32 | - |
AMP | pH and temperature conditions not mentioned | Salmonella enterica subsp. enterica serovar Typhimurium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required to form a complex with ATP and as a free cation | Salmonella enterica subsp. enterica serovar Typhimurium | |
Mn2+ | equally effective as Mg2+ | Salmonella enterica subsp. enterica serovar Typhimurium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme occurs in multiple states of aggregation | Salmonella enterica subsp. enterica serovar Typhimurium |
31000 | - |
5 * 31000, physical and electron microscopic studies | Salmonella enterica subsp. enterica serovar Typhimurium |
160000 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
LT-2 | - |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
Su 422 strain | - |
Salmonella enterica subsp. enterica serovar Typhimurium Su 422 | - |
Su 422 strain | - |
Purification (Comment) | Organism |
---|---|
extraction, streptomycin-heat treatment, ammonium sulfate and acid precipitation, affinity chromatography | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
130 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Storage Stability | Organism |
---|---|
-20°C, 50 mM potassium phosphate buffer, pH 7.5 after rapid freezing in CO2-acetone, quite stable | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose 5-phosphate | highly specific for ATP | Salmonella enterica subsp. enterica serovar Typhimurium | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | ribose product also known as PRPP | r | |
ATP + D-ribose 5-phosphate | maximal velocity of the reverse reaction is 9-10% of the forward reaction | Salmonella enterica subsp. enterica serovar Typhimurium | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | ribose product also known as PRPP | r | |
ATP + D-ribose 5-phosphate | highly specific for ATP | Salmonella enterica subsp. enterica serovar Typhimurium Su 422 | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | ribose product also known as PRPP | r | |
ATP + D-ribose 5-phosphate | maximal velocity of the reverse reaction is 9-10% of the forward reaction | Salmonella enterica subsp. enterica serovar Typhimurium Su 422 | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | ribose product also known as PRPP | r | |
CTP + D-ribose 5-phosphate | less than 3% of the activity with ATP | Salmonella enterica subsp. enterica serovar Typhimurium | CMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
CTP + D-ribose 5-phosphate | less than 3% of the activity with ATP | Salmonella enterica subsp. enterica serovar Typhimurium Su 422 | CMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
GTP + D-ribose 5-phosphate | less than 3% of the activity with ATP | Salmonella enterica subsp. enterica serovar Typhimurium | GMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
GTP + D-ribose 5-phosphate | less than 3% of the activity with ATP | Salmonella enterica subsp. enterica serovar Typhimurium Su 422 | GMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
ITP + D-ribose 5-phosphate | less than 3% of the activity with ATP | Salmonella enterica subsp. enterica serovar Typhimurium | IMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
UTP + D-ribose 5-phosphate | less than 3% of the activity with ATP | Salmonella enterica subsp. enterica serovar Typhimurium | UMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
UTP + D-ribose 5-phosphate | less than 3% of the activity with ATP | Salmonella enterica subsp. enterica serovar Typhimurium Su 422 | UMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
pentamer | 5 * 31000, physical and electron microscopic studies | Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.6 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
alpha,beta-methyleneATP | pH and temperature conditions not mentioned | Salmonella enterica subsp. enterica serovar Typhimurium | |
10 | - |
DL-1,4-Anhydroribitol 5-phosphate | pH and temperature conditions not mentioned | Salmonella enterica subsp. enterica serovar Typhimurium |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.05 | - |
IC50 of 0.05 mM | Salmonella enterica subsp. enterica serovar Typhimurium | Ca2+ |