Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine 5'-(beta,gamma-imido)-triphosphate | inhibition at 1 mM | Rattus norvegicus | |
ADP | one of the most effective | Rattus norvegicus | |
AMP | one of the most effective | Rattus norvegicus | |
dADP | - |
Rattus norvegicus | |
dATP | inhibition at 1 mM | Rattus norvegicus | |
GDP | one of the most effective | Rattus norvegicus | |
additional information | native enzyme is less sensitive to nucleotide inhibition than the major component of the enzyme, rPRSI | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0289 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 37ºC, native enzyme from liver | Rattus norvegicus | |
0.04 | - |
D-ribose 5-phosphate | pH 7.4, 37ºC, isoenzyme rPRSI | Rattus norvegicus | |
0.0402 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 37ºC, isoenzyme rPRSII | Rattus norvegicus | |
0.0418 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 37ºC, isoenzyme rPRSI | Rattus norvegicus | |
0.044 | - |
ATP | pH 7.4, 37ºC, isoenzyme rPRSI | Rattus norvegicus | |
0.049 | - |
ATP | pH 7.4, 37ºC, native enzyme from liver | Rattus norvegicus | |
0.06 | - |
ATP | pH 7.4, 37ºC, isoenzyme rPRSII | Rattus norvegicus | |
0.064 | - |
D-ribose 5-phosphate | pH 7.4, 37ºC, native enzyme from liver | Rattus norvegicus | |
0.073 | - |
D-ribose 5-phosphate | pH 7.4, 37ºC, isoenzyme rPRSII | Rattus norvegicus | |
0.117 | - |
AMP | pH 7.4, 37ºC, native enzyme from liver | Rattus norvegicus | |
0.123 | - |
AMP | pH 7.4, 37ºC, isoenzyme rPRSII | Rattus norvegicus | |
0.125 | - |
AMP | pH 7.4, 37ºC, isoenzyme rPRSI | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Rattus norvegicus | |
Mg2+ | activation of liver enzyme is almost maximal at 0.05 mM | Rattus norvegicus | |
Mg2+ | required to form a complex with ATP and as a free cation | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
500000 | 700000 | gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose 5-phosphate | Rattus norvegicus | the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
rat | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.45 | - |
native enzyme from liver, reverse reaction, pH 8.6 | Rattus norvegicus |
3.59 | - |
native enzyme from liver, reverse reaction, pH 7.4 | Rattus norvegicus |
4.24 | - |
isoenzyme rPRSII, reverse reaction, pH 8.6 | Rattus norvegicus |
4.5 | - |
isoenzyme rPRSI, reverse reaction, pH 8.6 | Rattus norvegicus |
7.48 | - |
isoenzyme rPRSI, reverse reaction, pH 7.4 | Rattus norvegicus |
8.04 | - |
isoenzyme rPRSII, reverse reaction, pH 7.4 | Rattus norvegicus |
8.3 | - |
isoenzyme rPRSII, pH 8.6 | Rattus norvegicus |
16.2 | - |
native enzyme from liver, pH 8.6 | Rattus norvegicus |
39.1 | - |
isoenzyme rPRSI, pH 8.6 | Rattus norvegicus |
Storage Stability | Organism |
---|---|
4ºC, without stabilizing agents, half-life of inactivation of 21, 8 and 1 hours for native enzyme, isoenzymes rPRSI and rPRSII, respectively | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose 5-phosphate | - |
Rattus norvegicus | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
ATP + D-ribose 5-phosphate | the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan | Rattus norvegicus | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
49 | - |
half-life of 250 min, 90 min and 0.5 min for native enzyme, isoenzymes rPRSI and rPRSII, respectively | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
rPRSII | Rattus norvegicus |
8.5 | - |
native enzyme from liver | Rattus norvegicus |
8.7 | - |
rPRSI | Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
66% of maximal activity, isoenzyme rPRSI | Rattus norvegicus |
7.4 | - |
62% of maximal activity, native enzyme from liver | Rattus norvegicus |
8.7 | - |
20% of maximal activity, isoenzyme rPRSII | Rattus norvegicus |