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Literature summary for 2.7.6.1 extracted from

  • Sonoda, T.; Kita, K.; Ishijima, S.; Ishizuka, T.; Ahmad, I.; Tatibana, M.
    Kinetic and regulatory properties of rat liver phosphoribosylpyrophosphate synthetase complex are partly distinct from those of isolated recombinant component catalytic subunits (1997), J. Biochem., 122, 635-640.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
adenosine 5'-(beta,gamma-imido)-triphosphate inhibition at 1 mM Rattus norvegicus
ADP one of the most effective Rattus norvegicus
AMP one of the most effective Rattus norvegicus
dADP
-
Rattus norvegicus
dATP inhibition at 1 mM Rattus norvegicus
GDP one of the most effective Rattus norvegicus
additional information native enzyme is less sensitive to nucleotide inhibition than the major component of the enzyme, rPRSI Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0289
-
5-phospho-alpha-D-ribose 1-diphosphate pH 7.4, 37ºC, native enzyme from liver Rattus norvegicus
0.04
-
D-ribose 5-phosphate pH 7.4, 37ºC, isoenzyme rPRSI Rattus norvegicus
0.0402
-
5-phospho-alpha-D-ribose 1-diphosphate pH 7.4, 37ºC, isoenzyme rPRSII Rattus norvegicus
0.0418
-
5-phospho-alpha-D-ribose 1-diphosphate pH 7.4, 37ºC, isoenzyme rPRSI Rattus norvegicus
0.044
-
ATP pH 7.4, 37ºC, isoenzyme rPRSI Rattus norvegicus
0.049
-
ATP pH 7.4, 37ºC, native enzyme from liver Rattus norvegicus
0.06
-
ATP pH 7.4, 37ºC, isoenzyme rPRSII Rattus norvegicus
0.064
-
D-ribose 5-phosphate pH 7.4, 37ºC, native enzyme from liver Rattus norvegicus
0.073
-
D-ribose 5-phosphate pH 7.4, 37ºC, isoenzyme rPRSII Rattus norvegicus
0.117
-
AMP pH 7.4, 37ºC, native enzyme from liver Rattus norvegicus
0.123
-
AMP pH 7.4, 37ºC, isoenzyme rPRSII Rattus norvegicus
0.125
-
AMP pH 7.4, 37ºC, isoenzyme rPRSI Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Rattus norvegicus
Mg2+ activation of liver enzyme is almost maximal at 0.05 mM Rattus norvegicus
Mg2+ required to form a complex with ATP and as a free cation Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
500000 700000 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-ribose 5-phosphate Rattus norvegicus the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
rat
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.45
-
native enzyme from liver, reverse reaction, pH 8.6 Rattus norvegicus
3.59
-
native enzyme from liver, reverse reaction, pH 7.4 Rattus norvegicus
4.24
-
isoenzyme rPRSII, reverse reaction, pH 8.6 Rattus norvegicus
4.5
-
isoenzyme rPRSI, reverse reaction, pH 8.6 Rattus norvegicus
7.48
-
isoenzyme rPRSI, reverse reaction, pH 7.4 Rattus norvegicus
8.04
-
isoenzyme rPRSII, reverse reaction, pH 7.4 Rattus norvegicus
8.3
-
isoenzyme rPRSII, pH 8.6 Rattus norvegicus
16.2
-
native enzyme from liver, pH 8.6 Rattus norvegicus
39.1
-
isoenzyme rPRSI, pH 8.6 Rattus norvegicus

Storage Stability

Storage Stability Organism
4ºC, without stabilizing agents, half-life of inactivation of 21, 8 and 1 hours for native enzyme, isoenzymes rPRSI and rPRSII, respectively Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-ribose 5-phosphate
-
Rattus norvegicus AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
ATP + D-ribose 5-phosphate the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan Rattus norvegicus AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
49
-
half-life of 250 min, 90 min and 0.5 min for native enzyme, isoenzymes rPRSI and rPRSII, respectively Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
rPRSII Rattus norvegicus
8.5
-
native enzyme from liver Rattus norvegicus
8.7
-
rPRSI Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.4
-
66% of maximal activity, isoenzyme rPRSI Rattus norvegicus
7.4
-
62% of maximal activity, native enzyme from liver Rattus norvegicus
8.7
-
20% of maximal activity, isoenzyme rPRSII Rattus norvegicus