2.7.4.9: dTMP kinase
This is an abbreviated version!
For detailed information about dTMP kinase, go to the full flat file.
Word Map on EC 2.7.4.9
Reaction
Synonyms
ATP: TMP phosphotransferase, Cdc8, deoxythymidine 5'-monophosphate kinase, deoxythymidine monophosphate kinase, dTMP kinase, dTMP kinases, dTMPK, human thymidine monophosphate kinase (hTMPK), kinase, thymidine monophosphate (phosphorylating), kinase, thymidylate (phosphorylating), ORF454, PfTMK, S. aureus thymidylate kinase, thymidine 5'-monophosphate kinase, thymidine kinase, thymidine monophosphate kinase, thymidylate kinase, thymidylate monophosphate kinase, thymidylic acid kinase, thymidylic kinase, TK1, TMK, TMP kinase, TMPK, TMPKmt, TTHA1607, TYMK
ECTree
2.7.4.9 dTMP kinaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 2.7.4.9 - dTMP kinase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + (E)-5-(2-bromovinyl)-deoxyuridine monophosphate
?
5% of the TMP efficiency
-
-
?
ATP + 2',3'-didehydro-2',3'-dideoxythymidine 5'-monophosphate
ADP + 2',3'-didehydro-2',3'-dideoxythymidine 5'-diphosphate
ATP + 2',3'-dideoxy-2',3'-didehydrothymidine 5'-monophosphate
ADP + 2',3'-dideoxy-2',3'-didehydrothymidine 5'-diphosphate
-
-
-
?
ATP + 3'-amino-3'-deoxythymidine 5'-monophosphate
ADP + 3'-amino-3'-deoxythymidine 5'-diphosphate
-
-
-
?
ATP + 3'-azido-2',3'-dideoxythymidine monophosphate
ADP + 3'-azido-2',3'-dideoxythymidine diphosphate
-
-
-
?
ATP + 3'-azido-3'-deoxythymidine 5'-monophosphate
ADP + 3'-azido-3'-deoxythymidine 5'-diphosphate
ATP + 3'-azido-3'-deoxythymidine monophosphate
ADP + 3'-azido-3'-deoxythymidine diphosphate
ATP + 3'-fluoro-3'-deoxythymidine 5'-monophosphate
ADP + 3'-fluoro-3'-deoxythymidine 5'-diphosphate
-
-
-
?
ATP + 5-bromo-2'-deoxyuridine 5'-monophosphate
ADP + 5-bromo-2'-deoxyuridine 5'-diphosphate
ATP + 5-fluorodeoxyuridine 5'-monophosphate
ADP + 5-fluorodeoxyuridine 5'-diphosphate
poor substrate
-
-
?
ATP + 5-iodo-2'-dUMP
ADP + 5-iodo-2'-dUDP
-
60% of the maximal activity with dTMP
-
-
?
ATP + 5-iodo-dUMP
ADP + 5-iodo-dUDP
-
34% of the activity with dTMP
-
-
?
ATP + D-2'-fluoroarabinosyl-5-methyldeoxyuridine 5'-monophosphate
ADP + D-2'-fluoroarabinosyl-5-methyldeoxyuridine 5'-diphosphate
172% of the activity with dTMP
-
-
?
ATP + L-2'-fluoroarabinosyl-5-methyldeoxyuridine 5'-monophosphate
ADP + L-2'-fluoroarabinosyl-5-methyldeoxyuridine 5'-diphosphate
poor substrate
-
-
?
ATP + N1-[(E)-4-dihydroxyphosphonyl-but-2-enyl]-5-chlorouracil
ADP + ?
-
-
-
?
ATP + N1-[(E)-4-dihydroxyphosphonyl-but-2-enyl]-5-fluorouracil
ADP + ?
-
-
-
?
ATP + N1-[(E)-5-dihydroxyphosphonyl-pent-2-enyl]-5-bromouracil
ADP + ?
-
-
-
?
ATP + N1-[(E)-5-dihydroxyphosphonyl-pent-2-enyl]-5-chlorouracil
ADP + ?
-
-
-
?
ATP + N1-[(E)-5-dihydroxyphosphonyl-pent-2-enyl]-5-fluorouracil
ADP + ?
-
-
-
?
dTMP + ATP
dTDP + ADP
-
TMPK catalyzes the conversion of dTMP to dTDP, using ATP as its preferred phosphoryl donor. Part of the biosynthetic pathway for deoxythymidine triphosphate (dTTP).
-
-
?
thymidine monophosphate + ATP
thymidine diphosphate + ADP
-
Thymidylate kinase (TMK) (E.C. 2.7.4.9) is a member of the NMP kinase family and catalyzes the phosphoryl transfer from the preferred phosphoryl donor, ATP, to thymidine monophosphate (TMP), yielding thymidine diphosphate (TDP). The TMK reaction is positioned at the junction of the de novo and salvage pathway of thymidine triphosphate (dTTP) synthesis, with TMK being the last specific enzyme for dTTP synthesis.
-
-
?
ATP + 2',3'-didehydro-2',3'-dideoxythymidine 5'-monophosphate
ADP + 2',3'-didehydro-2',3'-dideoxythymidine 5'-diphosphate
-
-
-
-
?
ATP + 2',3'-didehydro-2',3'-dideoxythymidine 5'-monophosphate
ADP + 2',3'-didehydro-2',3'-dideoxythymidine 5'-diphosphate
-
-
-
?
ATP + 2',3'-didehydro-2',3'-dideoxythymidine 5'-monophosphate
ADP + 2',3'-didehydro-2',3'-dideoxythymidine 5'-diphosphate
-
-
-
-
?
ATP + 3'-azido-3'-deoxythymidine 5'-monophosphate
ADP + 3'-azido-3'-deoxythymidine 5'-diphosphate
-
good substrate
-
-
?
ATP + 3'-azido-3'-deoxythymidine 5'-monophosphate
ADP + 3'-azido-3'-deoxythymidine 5'-diphosphate
-
-
-
?
ATP + 3'-azido-3'-deoxythymidine 5'-monophosphate
ADP + 3'-azido-3'-deoxythymidine 5'-diphosphate
-
i.e. AZT
-
-
?
ATP + 3'-azido-3'-deoxythymidine 5'-monophosphate
ADP + 3'-azido-3'-deoxythymidine 5'-diphosphate
-
no activity
-
-
?
ATP + 3'-azido-3'-deoxythymidine 5'-monophosphate
ADP + 3'-azido-3'-deoxythymidine 5'-diphosphate
-
Vmax is 100 times lower than with dTMP
-
-
?
ATP + 3'-azido-3'-deoxythymidine monophosphate
ADP + 3'-azido-3'-deoxythymidine diphosphate
-
-
-
?
ATP + 3'-azido-3'-deoxythymidine monophosphate
ADP + 3'-azido-3'-deoxythymidine diphosphate
-
-
-
?
ATP + 3'-azido-3'-deoxythymidine monophosphate
ADP + 3'-azido-3'-deoxythymidine diphosphate
-
-
-
-
?
ATP + 3'-azido-3'-deoxythymidine monophosphate
ADP + 3'-azido-3'-deoxythymidine diphosphate
-
-
-
?
ATP + 5-bromo-2'-deoxyuridine 5'-monophosphate
ADP + 5-bromo-2'-deoxyuridine 5'-diphosphate
-
-
-
-
?
ATP + 5-bromo-2'-deoxyuridine 5'-monophosphate
ADP + 5-bromo-2'-deoxyuridine 5'-diphosphate
-
-
-
-
?
ATP + dGMP
ADP + dGDP
-
dGMP is unable to displace TMP from its binding site
-
-
r
ATP + dTMP
ADP + dTDP
-
-
-
-
?
ATP + dTMP
ADP + dTDP
-
involved in dTTP metabolism, essential for cell proliferation
-
-
?
ATP + dTMP
ADP + dTDP
-
involved in TTP metabolism, required for the activation of clinically relevant thymidine analogs such as anti-HIV and anti-cancer prodrugs
-
-
?
ATP + dTMP
ADP + dTDP
-
-
-
-
?
ATP + dTMP
ADP + dTDP
-
involved in dTTP metabolism, essential for cell proliferation
-
-
?
ATP + dTMP
ADP + dTDP
involved in dTTP metabolism, essential for cell proliferation
-
-
?
ATP + dTMP
ADP + dTDP
involved in dTTP metabolism, essential for cell proliferation
-
-
r
ATP + dTMP
ADP + dTDP
-
reaction in both the de novo and salvage pathways of dTTP synthesis
-
-
?
ATP + dTMP
ADP + dTDP
specificity to the natural substrates ATP and dTMP
-
-
?
ATP + dTMP
ADP + dTDP
specificity to the natural substrates ATP and dTMP
-
-
?
ATP + dUMP
ADP + dUDP
-
12% of the activity with dTMP
-
?
ATP + dUMP
ADP + dUDP
-
12% of the activity with dTMP
-
?
ATP + TMP
ADP + TDP
-
TMP is able to partially displace dGMP from the binding site
-
-
r
dATP + dTMP
dADP + dTDP
26.9% of the activity with ATP
-
-
?
dATP + dTMP
dADP + dTDP
26.9% of the activity with ATP
-
-
?
dGTP + dTMP
dGDP + dTDP
Escherichia coli B / ATCC 11303
-
about 10% of maximal activity with ATP
-
-
?
additional information
?
-
no substrate: 3'-azidothymidine 5'-monophosphate
-
-
?
additional information
?
-
-
no substrate: 3'-azidothymidine 5'-monophosphate
-
-
?
additional information
?
-
-
multifunctional enzyme which also phosphorylates pyrimidine nucleosides, pyrimidine nucleoside monophosphates, and several nucleoside analogues
-
-
?
additional information
?
-
Herpes simplex virus
-
multifunctional enzyme which also phosphorylates pyrimidine nucleosides, pyrimidine nucleoside monophosphates, and several nucleoside analogues
-
-
?
additional information
?
-
direct correlation between the rate of phosphorylation of an NMP and its ability to induce a closing of the enzymes phosphate-binding loop
-
-
?
additional information
?
-
-
direct correlation between the rate of phosphorylation of an NMP and its ability to induce a closing of the enzymes phosphate-binding loop
-
-
?
additional information
?
-
no activity with cidofovir, N1-[(E)-3-dihydroxyphosphonyl-prop-2-enyl]-uracil, N1-[(E)-3-dihydroxyphosphonyl-prop-2-enyl]-5-chlorouracil, N1-[(E)-3-dihydroxyphosphonyl-prop-2-enyl]-5-bromouracil, N1-[(E)-3-dihydroxyphosphonyl-prop-2-enyl]-5-fluoro-uracil, and N1-[(E)-3-dihydroxyphosphonyl-prop-2-enyl]-thymine
-
-
?
additional information
?
-
-
no activity with cidofovir, N1-[(E)-3-dihydroxyphosphonyl-prop-2-enyl]-uracil, N1-[(E)-3-dihydroxyphosphonyl-prop-2-enyl]-5-chlorouracil, N1-[(E)-3-dihydroxyphosphonyl-prop-2-enyl]-5-bromouracil, N1-[(E)-3-dihydroxyphosphonyl-prop-2-enyl]-5-fluoro-uracil, and N1-[(E)-3-dihydroxyphosphonyl-prop-2-enyl]-thymine
-
-
?
additional information
?
-
-
thymidine/thymidylate multifunctional kinase
-
-
?
additional information
?
-
-
Cdc8 does not phosphorylate dAMP, dGMP and dCMP
-
-
?
additional information
?
-
-
poxvirus thymidylate kinase can also accept bulky analogues such as dGMP or 5-bromo-vinyl-dUMP as substrates
-
-
?
additional information
?
-
-
sequential ordered bi-bi reaction mechanism. The enzyme additionally shows nucleoside-diphosphate kinase activity, reaction of EC 2.7.4.6, using dTDP as a substrate to produce dTTP
-
-
?
additional information
?
-
maximal activity is achieved at equimolar concentrations of ATP and Mg2+
-
-
?
additional information
?
-
maximal activity is achieved at equimolar concentrations of ATP and Mg2+
-
-
?
