2.7.4.9: dTMP kinase

This is an abbreviated version!
For detailed information about dTMP kinase, go to the full flat file.

Reaction

Synonyms

ATP: TMP phosphotransferase, Cdc8, deoxythymidine 5'-monophosphate kinase, deoxythymidine monophosphate kinase, dTMP kinase, dTMP kinases, dTMPK, human thymidine monophosphate kinase (hTMPK), kinase, thymidine monophosphate (phosphorylating), kinase, thymidylate (phosphorylating), ORF454, PfTMK, S. aureus thymidylate kinase, thymidine 5'-monophosphate kinase, thymidine kinase, thymidine monophosphate kinase, thymidylate kinase, thymidylate monophosphate kinase, thymidylic acid kinase, thymidylic kinase, TK1, TMK, TMP kinase, TMPK, TMPKmt, TTHA1607, TYMK

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.4 Phosphotransferases with a phosphate group as acceptor

                2.7.4.9 dTMP kinase

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Results	in table
1	Activating Compound
45422	AA Sequence
12	Application
1	CAS Registry Number
29	Cloned(Commentary)
9	Cofactor
23	Crystallization (Commentary)
179	Disease/ Diagnostics
23	General Information
8	General Stability
178	IC50 Value
502	Inhibitors
18	kcat/KM [mM/s]
198	Ki Value [mM]
101	KM Value [mM]
15	Localization
29	Metals/Ions
21	Molecular Weight [Da]
29	Natural Substrates/ Products (Substrates)
298	Organism
11	Pathway
232	PDB ID
9	pH Optimum
6	pH Range
3	pH Stability
29	Protein Variants
27	Purification (Commentary)
3	Reaction
4	Reaction Type
107	Reference
1	Renatured (Commentary)
23	Source Tissue
11	Specific Activity [micromol/min/mg]
4	Storage Stability
215	Substrates and Products (Substrate)
21	Subunits
99	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
1	Temperature Range [°C]
14	Temperature Stability [°C]
63	Turnover Number [1/s]

General Stability

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General Stability on EC 2.7.4.9 - dTMP kinase

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GENERAL STABILITY

ORGANISM

UNIPROT

LITERATURE

dissociation of the native dimeric species of the enzyme occurs at an urea concentration of around 4 M, leading to the accumulation of partially folded monomers that unfold totally at urea concentrations above 5.5 M

Mycobacterium tuberculosis

-

645207

dTDP and dTTP are effective as enzyme stabilizer

Mus musculus

-

645188

midpoint transition is 3.0 M urea for the wild-type enzyme and 3.3 M for the G146A mutant enzyme

Escherichia coli

-

645202

the canonical enzyme is stable and its activity is not affected by the presence of NaCl

Homo sapiens

P23919

759870

the enzyme from outer mitochondrial membrane is unstable and loses activity rapidly, it also loses activity in the presence of a high concentration of NaCl

Homo sapiens

P23919

759870

the enzyme is very labile and can be stabilized for long periods of time by its substrate thymidine 5'-monophosphate in the presence of 2-mercaptoethanol

Gallus gallus

-

645194

the enzyme is very unstable and may be stabilized in the presence of 2-mercaptoethanol with dTMP, dTDP, or ADP but not with ATP, MgATP2- or mercaptoethanol alone

Mus musculus

-

645196

the stability of the enzyme is maintained during purification by the constant presence of dTMP and 2-mercaptoethanol and by the elimination of substrate-destoying phosphatase activity

Mus musculus

-

645188