2.7.4.9: dTMP kinase
This is an abbreviated version!
For detailed information about dTMP kinase, go to the full flat file.
Reaction
Synonyms
ATP: TMP phosphotransferase, Cdc8, deoxythymidine 5'-monophosphate kinase, deoxythymidine monophosphate kinase, dTMP kinase, dTMP kinases, dTMPK, human thymidine monophosphate kinase (hTMPK), kinase, thymidine monophosphate (phosphorylating), kinase, thymidylate (phosphorylating), ORF454, PfTMK, S. aureus thymidylate kinase, thymidine 5'-monophosphate kinase, thymidine kinase, thymidine monophosphate kinase, thymidylate kinase, thymidylate monophosphate kinase, thymidylic acid kinase, thymidylic kinase, TK1, TMK, TMP kinase, TMPK, TMPKmt, TTHA1607, TYMK
ECTree
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Purification
Purification on EC 2.7.4.9 - dTMP kinase
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glutathione-Sepharose column chromatography and Mono Q column chromatography
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nickel HisTrap column chromatography and Superdex 75 gel filtration
partial
the stability of the enzyme is maintained during purification by the constant presence of dTMP and 2-mercaptoethanol and by the elimination of substrate-destoying phosphatase activity
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truncated recombinant enzyme, full length enzyme could not be crystallized
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