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Literature summary for 2.7.4.9 extracted from

  • Gardberg, A.; Shuvalova, L.; Monnerjahn, C.; Konrad, M.; Lavie, A.
    Structural basis for the dual thymidine and thymidylate kinase activity of herpes thymidine kinases (2003), Structure, 11, 1265-1277.
    View publication on PubMed
Search for more literature for 2.7.4.9

Cloned(Commentary)

Cloned (Comment) Organism
full length and truncated enzyme lacking the first 22 amino acids expressed in Escherichia coli BL21 C41 (DE3) Equid alphaherpesvirus 4

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method Equid alphaherpesvirus 4

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36500
-
 2 * 36500, calculated from the deduced amino acid sequence, crystal structure analysis Equid alphaherpesvirus 4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + thymidine Herpes simplex virus
-
 ADP + TMP
-
 ?
ATP + thymidine Equid alphaherpesvirus 4
-
 ADP + TMP
-
 ?
ATP + TMP Herpes simplex virus
-
 ADP + TDP
-
 ?
ATP + TMP Equid alphaherpesvirus 4
-
 ADP + TDP
-
 ?
additional information Herpes simplex virus multifunctional enzyme which also phosphorylates pyrimidine nucleosides, pyrimidine nucleoside monophosphates, and several nucleoside analogues ?
-
 ?
additional information Equid alphaherpesvirus 4 multifunctional enzyme which also phosphorylates pyrimidine nucleosides, pyrimidine nucleoside monophosphates, and several nucleoside analogues ?
-
 ?

Organism

Organism UniProt Comment Textmining
Equid alphaherpesvirus 4
-
 virus type 4
-
Herpes simplex virus
-
 virus type 1
-

Purification (Commentary)

Purification (Comment) Organism
truncated recombinant enzyme, full length enzyme could not be crystallized Equid alphaherpesvirus 4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + thymidine
-
 Herpes simplex virus ADP + TMP
-
 ?
ATP + thymidine
-
 Equid alphaherpesvirus 4 ADP + TMP
-
 ?
ATP + TMP
-
 Herpes simplex virus ADP + TDP
-
 ?
ATP + TMP
-
 Equid alphaherpesvirus 4 ADP + TDP
-
 ?
additional information multifunctional enzyme which also phosphorylates pyrimidine nucleosides, pyrimidine nucleoside monophosphates, and several nucleoside analogues Herpes simplex virus ?
-
 ?
additional information multifunctional enzyme which also phosphorylates pyrimidine nucleosides, pyrimidine nucleoside monophosphates, and several nucleoside analogues Equid alphaherpesvirus 4 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 36500, calculated from the deduced amino acid sequence, crystal structure analysis Equid alphaherpesvirus 4