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Literature summary for 2.7.4.9 extracted from

  • Munier-lehmann, H.; Chaffotte, A.; Pochet, S.; Labesse, G.
    Thymidylate kinase of Mycobacterium tuberculosis: a chimera sharing properties common to eukaryotic and bacterial enzymes (2001), Protein Sci., 10, 1195-1205.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.4.9

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Mycobacterium tuberculosis

General Stability

General Stability Organism
dissociation of the native dimeric species of the enzyme occurs at an urea concentration of around 4 M, leading to the accumulation of partially folded monomers that unfold totally at urea concentrations above 5.5 M Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
3'-azido-3'-deoxythymidine 5'-monophosphate potent competitive inhibitor Mycobacterium tuberculosis
3'-azido-3'-deoxythymidine monophosphate
-
 Mycobacterium tuberculosis
5-methyl iso-dCMP competitive Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0045
-
 dTMP pH 7.4, 30°C Mycobacterium tuberculosis
0.1
-
 ATP pH 7.4, 30°C Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22635
-
 2 * 22635, electrospray ionization mass spectrometry Mycobacterium tuberculosis
46600
-
 sedimentation equilibrium ultracentrifugation Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + dTMP
-
 Mycobacterium tuberculosis ADP + dTDP
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 22635, electrospray ionization mass spectrometry Mycobacterium tuberculosis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 10 min, 50% inactivation, irreversible Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.02
-
 3'-azido-3'-deoxythymidine monophosphate pH 7.4, 30°C Mycobacterium tuberculosis
0.13
-
 5-methyl iso-dCMP pH 7.4, 30°C Mycobacterium tuberculosis